Anesthesia Induction’s Effect on Pediatric Surgery under Parental Accompany: A Comprehensive Meta-Analysis of Randomized Controlled Trials

Objective: To evaluate the effect of anesthesia induction under parental company for children receiving general anesthesia. Methods: The RCT results were collected on children’s preoperative anxiety, anesthesia coordination, anesthesia and recovery agitation, postoperative pain and parental preoperative anxiety intervention with or without parental accompany using RevMan 5.3 software based on Chinese and English database. We searched Cochrane Library, Pubmed, Medline, EMbase, Sciencedirect, SpringerLink, China Biomedical, CNKI, Wanfang, Weipu and other databases, and included 15 articles (5 Chinese, 10 English) with 1390 samples size, containing 700 cases of control group and 690 cases of testing group. Results: Our results showed that parents’ and children’s anxiety level, the incidence of restlessness during anesthesia induction and wake-up period were reduced, while the anesthesia coordination was enhanced and the pain of children after wake-up was alleviated under parental accompany. Conclusion: The risk and cost-free intervention of parental accompany could be recommended as appropriate in hospitals for its contribution to children’s surgery effect.

一例X染色体结构重排导致DMD疾病

假肥大型肌营养不良(Duchenne/Becker muscular dystrophy,DMD/BMD)是一种最常见的进行性肌营养不良疾病,呈X-连锁隐性遗传,主要由DMD基因的缺失、重复及点突变所致,极少数病例是由于染色体结构重排破坏了DMD基因而引起疾病的发生。本文报告了1例经多重连接探针扩增技术(multiplex ligation-dependent probe amplification,MLPA)和下一代测序检测后原因未明的、具有典型症状的DMD患者。采用核型分析、FISH分析及三代测序、Sanger测序综合分析发现,患者存在母源性的X染色体臂间倒位(Chr.X:g.[31939463–31939465del;31939466–131765063 inv;131765064–131765067del])半合子变异。由于该变异破坏了DMD基因和HS6ST2基因,因此推测该变异是患者发病的遗传学病因。患者表现肌无力等典型的DMD症状,没有明显的Paganini-Miozzo综合征相关症状。本病例的明确诊断,提示结构重排破坏DMD基因也是导致DMD重要原因之一;常规遗传学检测阴性的患者应考虑核型分析、FISH验证等结构重排变异检测技术,通过三代测序技术能确定大概的重排断点位置,Sanger测序可明确断点区域序列。本病例报告通过明确的遗传学诊断为该患者所在家庭进行生殖干预、降低再生育风险提供了诊疗基础。

Investigation of the apoptotic mechanism in hippocampal and retinal neurons of db/db mice induced by Dihuangyinzi decoction

In the present study,we utilized db/db mice to investigate the mechanisms of apoptosis in brain and retinal neurons.A total of 30 male db/db mice aged 8–9 weeks were randomly assigned to the model group,Dihuangyinzi decoction(DHYZ)group(30.03 g/kg),and metformin(MET)group(0.58 g/kg),with 10 mice in each group.The control group comprised 10 db/m mice of the same background and age.Paired-associate learning(PAL)tests were conducted to evaluate the learning and memory functions of the mice.Histological assessments,including Hematoxylin-Eosin(H&E)and Nissl staining,were employed to observe changes in nerve cells in the hippocampus and retina.Immunohistochemistry and real-time fluorescence quantitative PCR were employed to detect the positive expression of anti-apoptotic factor Bcl-2 and pro-apoptotic factor Bax at the protein and mRNA levels in the hippocampus and retina.Western blotting analysis was adopted to assess protein expression levels of JNK,p-JNK,p38 MAPK,and p-p38 MAPK.Results revealed a significant decline in the correct rate of PAL test results in the model group(P<0.001),accompanied by increased reaction and delay times(P<0.001)and higher blood glucose levels(P<0.001).H&E and Nissl staining indicated a reduction in the number of nerve cells in the CA1 area of the hippocampus in the model group,with scattered arrangement and decreased Nissl corpuscles.Positive expression and mRNA expression of Bax in the hippocampus and retina increased significantly(P<0.001),while positive expression and mRNA expression of Bcl-2 decreased(P<0.001).Protein expression levels of p-JNK/JNK and p-p38 MAPK/p38 MAPK showed a significant increase(P<0.001).The DHYZ and MET groups exhibited enhanced accuracy in PAL experiments(P<0.05),decreased reaction time and delay time(P<0.05),and reduced blood glucose levels(P<0.05).The number of neurons in the CA1 region of the hippocampus increased,morphological structure improved,and the arrangement of the hippocampal structure became more orderly.Additionally,no obvious vacuolization was observed in the neuronal cell layer.The number of Nissl bodies increased,and the layers of the retina were closely arranged,with an increase in the number of Nissl bodies.Positive expressions of Bax in the hippocampus and retina,both at the protein and mRNA levels,decreased significantly(P<0.001),while positive expressions of Bcl-2 and its mRNA increased(P<0.01,P<0.001).Furthermore,the protein expressions of p-JNK/JNK and p-p38 MAPK/p38 MAPK decreased significantly(P<0.01).This study suggested that DHYZ decoction could inhibit the JNK/p38 MAPK signaling pathway,thereby increasing the anti-apoptotic factor Bcl-2 and reducing the expression of the pro-apoptotic factor Bax,consequently inhibiting apoptosis in the hippocampal CA1 region and retinal neurons of db/db mice.

Deciphering the therapeutic mechanisms of Fructus Ligustri Lucidi on diabetic nephropathy via bioinformatics analysis

This study aimed to investigate the potential mechanisms underlying the therapeutic effects of Fructus Ligustri Lucidi in the treatment of diabetic nephropathy(DN)through bioinformatics analysis.The research commenced with a comprehensive database search for DN-associated datasets and gene targets of Fructus Ligustri Lucidi.Subsequently,the ssGSEA method was employed to score the drug target gene sets,and based on these scores,subjects were categorized.Further analysis involved differential analysis and enrichment analysis techniques to elucidate differentially expressed genes(DEGs)and associated pathways between the two groups.Based on the ssGSEA scoring results,the data were stratified into two groups:the"High NZZ group"and the"Low NZZ group".Through differential analysis,a total of 18 DEGs were identified,comprising 14 upregulated genes(EGR1,CCL2,CDH6,VCAN-AS1,VCAN,C3,MMP7,RNASE6,C1QC,MOXD1,APOC1,SFRP2,CCL21,and LUM)and four downregulated genes(OTTHUMG00000152025,RERG,B3GALT2,and NELL1).Notably,several genes exhibited concurrent expression patterns in both the DN and High NZZ groups,including VCAN-AS1,CCL21,VCAN,MOXD1,CCL2,SFRP2,MMP7,C3,RNASE6,LUM,C1QC,APOC1,and CDH6.Enrichment analysis revealed significant enrichment in pathways related to"regulation of apoptotic cell clearance"and"granulocyte chemotaxis",among others.These findings highlighted the potential value of Fructus Ligustri Lucidi in the treatment of DN and unveiled novel potential targets for prognosis and therapy in DN patients.This study offered promising avenues for enhancing both the prognosis and treatment outcomes for individuals affected by this condition.

Status and Development Countermeasures of Moderate-scale Management of Grain and Oil in Nanchong City

This paper introduces the development status of moderate-scale management of grain and oil in Nanchong,analyzes the existing problems in production and management,puts forward some suggestions in terms of fund,science and technology and industrial structure,and lists some cases for reference,so as to provide a direction for the operation of grain and oil in Nanchong.

Synthesis and dissociation of soliton molecules in parallel optical-soliton reactors

Mode-locked lasers have been widely used to explore interactions between optical solitons,including bound-soliton states that may be regarded as“photonic molecules”.Conventional mode-locked lasers normally,however,host at most only a few solitons,which means that stochastic behaviours involving large numbers of solitons cannot easily be studied under controlled experimental conditions.Here we report the use of an optoacoustically mode-locked fibre laser to create hundreds of temporal traps or“reactors”in parallel,within each of which multiple solitons can be isolated and controlled both globally and individually using all-optical methods.We achieve on-demand synthesis and dissociation of soliton molecules within these reactors,in this way unfolding a novel panorama of diverse dynamics in which the statistics of multi-soliton interactions can be studied.The results are of crucial importance in understanding dynamical soliton interactions and may motivate potential applications for all-optical control of ultrafast light fields in optical resonators.

CECAV-DNN:Collective Ensemble Comparison and Visualization using Deep Neural Networks

We propose a deep learning approach to collectively compare two or multiple ensembles,each of which is a collection of simulation outputs.The purpose of collective comparison is to help scientists understand differences between simulation models by comparing their ensemble simulation outputs.However,the collective comparison is non-trivial because the spatiotemporal distributions of ensemble simulation outputs reside in a very high dimensional space.To this end,we choose to train a deep discriminative neural network to measure the dissimilarity between two given ensembles,and to identify when and where the two ensembles are different.We also design and develop a visualization system to help users understand the collective comparison results based on the discriminative network.We demonstrate the effectiveness of our approach with two real-world applications,including the ensemble comparison of the community atmosphere model(CAM)and the rapid radiative transfer model for general circulation models(RRTMG)for climate research,and the comparison of computational fluid dynamics(CFD)ensembles with different spatial resolutions.

Stimulated Brillouin scattering in chiral photonic crystal fiber

Stimulated Brillouin scattering(SBS)has many applications;for example,in sensing,microwave photonics,and signal processing.Here,we report the first experimental study of SBS in chiral photonic crystal fiber(PCF),which displays optical activity and robustly maintains circular polarization states against external perturbations.As a result,circularly polarized pump light is cleanly backscattered into a Stokes signal with the orthogonal circular polarization state,as is required by angular momentum conservation.By comparison,untwisted PCF generates a Stokes signal with an unpredictable polarization state,owing to its high sensitivity to external perturbations.We use chiral PCF to realize a circularly polarized continuous-wave Brillouin laser.The results pave the way for a new generation of stable circularly polarized SBS systems with applications in quantum manipulation,optical tweezers,optical gyroscopes,and fiber sensors.

USEVis:Visual analytics of attention-based neural embedding in information retrieval

Neural attention-based encoders,which effectively attend sentence tokens to their associated context without being restricted by long-term distance or dependency,have demonstrated outstanding performance in embedding sentences into meaningful representations(embeddings).The Universal Sentence Encoder(USE)is one of the most well-recognized deep neural network(DNN)based solutions,which is facilitated with an attention-driven transformer architecture and has been pre-trained on a large number of sentences from the Internet.Besides the fact that USE has been widely used in many downstream applications,including information retrieval(IR),interpreting its complicated internal working mechanism remains challenging.In this work,we present a visual analytics solution towards addressing this challenge.Specifically,focused on semantics and syntactics(concepts and relations)that are critical to domain clinical IR,we designed and developed a visual analytics system,i.e.,USEVis.The system investigates the power of USE in effectively extracting sentences’semantics and syntactics through exploring and interpreting how linguistic properties are captured by attentions.Furthermore,by thoroughly examining and comparing the inherent patterns of these attentions,we are able to exploit attentions to retrieve sentences/documents that have similar semantics or are closely related to a given clinical problem in IR.By collaborating with domain experts,we demonstrate use cases with inspiring findings to validate the contribution of our work and the effectiveness of our system.