The fidelity of DNA transcription catalyzed by RNA polymerase(RNAP)has long been an important issue in biology.Experiments have revealed that RNAP can incorporate matched nucleotides selectively and proofread the inco...The fidelity of DNA transcription catalyzed by RNA polymerase(RNAP)has long been an important issue in biology.Experiments have revealed that RNAP can incorporate matched nucleotides selectively and proofread the incorporated mismatched nucleotides.However,systematic theoretical researches on RNAP fidelity are still lacking.In the last decade,several theories on RNA transcription have been proposed,but they only handled highly simplified models without considering the high-order neighbor effects and the oligonucleotides cleavage both of which are critical for the overall fidelity.In this paper,we regard RNA transcription as a binary copolymerization process and calculate the transcription fidelity by the steady-state copolymerization theory recently proposed by us for DNA replication.With this theory,the more realistic models considering higher-order neighbor effects,oligonucleotides cleavage,multi-step incorporation and multi-step cleavage can be rigorously handled.展开更多
基金The authors thank the financial support by National Natural Science Foundation of China(No.11675180,11774358)the CAS Strategic Priority Research Program(No.XDA17010504)+1 种基金Key Research Program of Frontier Sciences of CAS(No.Y7Y1472Y61)Research Fund of Wenzhou Institute CAS(No.WIUCASYJ2020004,WIUCASQD2020009).
文摘The fidelity of DNA transcription catalyzed by RNA polymerase(RNAP)has long been an important issue in biology.Experiments have revealed that RNAP can incorporate matched nucleotides selectively and proofread the incorporated mismatched nucleotides.However,systematic theoretical researches on RNAP fidelity are still lacking.In the last decade,several theories on RNA transcription have been proposed,but they only handled highly simplified models without considering the high-order neighbor effects and the oligonucleotides cleavage both of which are critical for the overall fidelity.In this paper,we regard RNA transcription as a binary copolymerization process and calculate the transcription fidelity by the steady-state copolymerization theory recently proposed by us for DNA replication.With this theory,the more realistic models considering higher-order neighbor effects,oligonucleotides cleavage,multi-step incorporation and multi-step cleavage can be rigorously handled.
