Fluorescence photoswitch is becoming increasingly desirable for many applications,but its controllable fabrication still remains challenging yet.In this paper,a new strategy is proposed to fabricate fluorescence photo...Fluorescence photoswitch is becoming increasingly desirable for many applications,but its controllable fabrication still remains challenging yet.In this paper,a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore.As an example,we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye(CMTE)and red-emitting fluorescent gold nanoclusters(AuNCs)into the specific domains of bovine serum albumin(BSA)in a highly controllable manner.Detailed spectral and photophysical characterisation showed that CMTE well-retains the photochromic properties within the CMTE–BSA–AuNC construct,although its photoconversion rate is slightly retarded.Different from previously reported photoswitches,the fluorescence of the present system is mainly modulated via the inner filter effect(IFE)mechanism,which exhibits high switching efficiency with an on-off ratio up to 90%,reversible fluorescence response and good antifatigue performance.This work provides a new,generable albumin protein-assisted strategy of fabricating advanced fluorescence photoswitch,which can find wide applications in biological,optical and information fields.展开更多
The fascinating luminescence properties of gold nanoclusters(AuNCs) have drawn considerable research interests,and been widely harnessed for a wide range of applications.However,a fundamental understanding towards lig...The fascinating luminescence properties of gold nanoclusters(AuNCs) have drawn considerable research interests,and been widely harnessed for a wide range of applications.However,a fundamental understanding towards ligand density’s role in the luminescence properties of these ultrasmall AuNCs remains unclear yet.In this communication,through systematic investigation of surface chemistries of glutathione-protected Au NCs(GSH-Au NCs) with diffe rent density of GSH as well as other thiolates,it is discovered that the density of surface ligands can significantly regulate the luminescence properties of AuNCs.Fluorescence lifetime spectroscopy and X-ray photoelectron spectroscopy showed that AuNCs with a higher density of electron-rich ligands facilitate their luminescence generation.Moreover,differences in the surface coverage of AuNCs can also affect their interactions with foreign species,as illustrated by significantly different fluorescence quenching capability of GSH-AuNCs with different ligand density towards Hg^(2+).This study provides new insight into the intriguing luminescence properties of metal NCs,which is hoped to stimulate further research on the design of metal NCs with strong luminescence and sensitive/specific responses for promising optoelectronic,sensing and imaging applications.展开更多
基金Natural Science Foundation of Chongqing,Grant/Award Number:cstc2021jcyj-msxmX0980Fundamental Research Fund for the Central University,Grant/Award Number:3102019jcc005+1 种基金Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University,Grant/Award Number:CX2021053Research Fund of the State Key Laboratory of Solidification Processing,Grant/Award Number:2020-QZ-01。
文摘Fluorescence photoswitch is becoming increasingly desirable for many applications,but its controllable fabrication still remains challenging yet.In this paper,a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore.As an example,we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye(CMTE)and red-emitting fluorescent gold nanoclusters(AuNCs)into the specific domains of bovine serum albumin(BSA)in a highly controllable manner.Detailed spectral and photophysical characterisation showed that CMTE well-retains the photochromic properties within the CMTE–BSA–AuNC construct,although its photoconversion rate is slightly retarded.Different from previously reported photoswitches,the fluorescence of the present system is mainly modulated via the inner filter effect(IFE)mechanism,which exhibits high switching efficiency with an on-off ratio up to 90%,reversible fluorescence response and good antifatigue performance.This work provides a new,generable albumin protein-assisted strategy of fabricating advanced fluorescence photoswitch,which can find wide applications in biological,optical and information fields.
基金supported by the National Natural Science Foundation of China (No.21705129)the Fundamental Research Fund for the Central University (Nos.3102019jcc005,3102019GHJD001)the Research Fund of the State Key Laboratory of Solidification Processing (NPU),China (No.2020-QZ-01)。
文摘The fascinating luminescence properties of gold nanoclusters(AuNCs) have drawn considerable research interests,and been widely harnessed for a wide range of applications.However,a fundamental understanding towards ligand density’s role in the luminescence properties of these ultrasmall AuNCs remains unclear yet.In this communication,through systematic investigation of surface chemistries of glutathione-protected Au NCs(GSH-Au NCs) with diffe rent density of GSH as well as other thiolates,it is discovered that the density of surface ligands can significantly regulate the luminescence properties of AuNCs.Fluorescence lifetime spectroscopy and X-ray photoelectron spectroscopy showed that AuNCs with a higher density of electron-rich ligands facilitate their luminescence generation.Moreover,differences in the surface coverage of AuNCs can also affect their interactions with foreign species,as illustrated by significantly different fluorescence quenching capability of GSH-AuNCs with different ligand density towards Hg^(2+).This study provides new insight into the intriguing luminescence properties of metal NCs,which is hoped to stimulate further research on the design of metal NCs with strong luminescence and sensitive/specific responses for promising optoelectronic,sensing and imaging applications.