Discovery of the radio-protecting effect of Ecliptae Herba,its constituents and targeting p53-mediated apoptosis in vitro and in vivo

Radiation protection drugs are often accompanied by toxicity,even amifostine,which has been the dominant radio-protecting drug for nearly 30 years.Furthermore,there is no therapeutic drug for radiation-induced intestinal injury(RⅢ).This paper intends to find a safe and effective radioprotecting ingredient from natural sources.The radio-protecting effect of Ecliptae Herba(EHE) was discovered preliminarily by antioxidant experiments and the mouse survival rate after137Cs irradiation.EHE components and blood substances in vivo were identified through UPLC-Q-TOF.The correlation network of “natural components in EHE-constituents migrating to blood-targets-pathways” was established to predict the active components and pathways.The binding force between potential active components and targets was studied by molecular docking,and the mechanism was further analyzed by Western blotting,cellular thermal shift assay(CETSA),and Ch IP.Additionally,the expression levels of Lgr5,Axin2,Ki67,lysozyme,caspase-3,caspase-8,8-OHd G,and p53 in the small intestine of mice were detected.It was found for the first time that EHE is active in radiation protection and that luteolin is the material basis of this protection.Luteolin is a promising candidate for RⅢ.Luteolin can inhibit the p53 signaling pathway and regulate the BAX/BCL2 ratio in the process of apoptosis.Luteolin could also regulate the expression of multitarget proteins related to the same cell cycle.

Author correction to‘Discovery of the radioprotecting effect of ecliptae herba,its constituents and targeting p53-mediated apoptosis in vitro and in vivo’[Acta Pharm Sin B 13(2023)1216-1230]

The authors regret that there is an error in the article Fig.4B due to the mistake of copying and pasting in the process of assembling figures and negligence in the proofreading.Although it does not affect the conclusion,it is an obvious error.The authors have now modified as below.The authors apologize for any inconvenience caused to the journal and readers.

Molecular mechanisms of isoflavone puerarin against cardiovascular diseases:What we know and where we go

Puerarin(Pue),known as a phytoestrogen,has salient bioactivities and is promising against cardiovascular diseases.This article summarizes the underlying molecular mechanisms of Pue in treating cardiovascular diseases,especially regulating the intracellular signal transduction,influencing ion channels,modulating the expression of micro RNA,and impacting on the autophagy,which are mainly involved in the inflammatory signaling pathways,fatty acid/lipid metabolism,oxidative stress,apoptosis,and the like.The protective effect of Pue against cardiovascular diseases mainly involves attenuating the myocardial injury and decreasing the myocardial fibrosis,improving the myocardial ischemia/reperfusion injury,as well as inhibiting the myocardial hypertrophy and atherosclerosis.The molecular mechanisms of Pue’s cardiovascular protective effects for the first time and comment on the state-of-the-art research methods and principles of Pue’s regulation of small molecules were reviewed,so as to provide the rationale for its basic research and clinical applications.