TET2合并DNMT3A突变及其他共存基因突变对急性髓系白血病患者预后的影响

目的:探究TET2合并DNMT3A突变及其他共存基因突变对成人非M3型急性髓系白血病(acute myeloid leukemia,AML)患者预后的影响。方法:回顾性分析2018年1月至2021年9月于南昌大学第一附属医院确诊的初治且行血液肿瘤相关突变基因外显子二代测序检测的512例成人AML(非M3型)患者的临床资料,分析患者的临床特征、疗效及生存情况。结果:本研究共纳入110例AML患者,TET2突变组64例,DNMT3A单突变组46例。男性50例(45.5%),中位年龄54(15~79)岁。TET2基因突变频率为12.5%(64/512),98.4%(63/64)患者突变基因数≥2个,每例患者平均合并5.2个突变基因。NPM1(43.8%)、DNMT3A(42.2%)、FLT3-ITD/TKD(40.6%)、CEBPA(26.6%)、TTN(20.3%)为TET2突变常见的共存突变基因。TET2合并DNMT3A突变患者初次诱导完全缓解(complete response,CR)率为46.2%,略低于TET2单突变患者的76.9%(P=0.077),与DNMT3A单突变患者无显著性差异(P=0.952)。TET2合并DNMT3A突变患者的中位总生存(median overall survival,mOS)时间为9.5个月,明显低于TET2单突变患者(P=0.002),而与DNMT3A单突变患者无显著性差异(P=0.414)。三者的中位无复发生存(median relapse-free survival,mRFS)时间无显著性差异(P>0.05)。在TET2突变背景下,合并K/NRAS突变患者的CR率为28.6%,明显低于无K/NRAS突变患者的75.0%(P=0.030),合并FLT3-ITD突变患者的mOS明显短于无FLT3-ITD突变患者(P=0.030)。多因素分析显示年龄≥60岁、合并FLT3-ITD突变、初次诱导未达CR是影响TET2突变AML患者总生存时间(overall survival,OS)的独立危险因素,DNMT3A突变不影响TET2突变患者OS。结论:TET2突变是AML患者常见突变,且常合并共存基因突变。共存基因突变与TET2突变共同影响AML患者预后。基于二代测序的基因突变检测对指导AML精确分层及精准治疗具有重要意义。

Relation-Aware Entity Matching Using Sentence-BERT

A key aspect of Knowledge fusion is Entity Matching.The objective of this study was to investigate how to identify heterogeneous expressions of the same real-world entity.In recent years,some representative works have used deep learning methods for entity matching,and these methods have achieved good results.However,the common limitation of these methods is that they assume that different attribute columns of the same entity are independent,and inputting the model in the form of paired entity records will cause repeated calculations.In fact,there are often potential relations between different attribute columns of different entities.These relations can help us improve the effect of entity matching,and can perform feature extraction on a single entity record to avoid repeated calculations.To use attribute relations to assist entity matching,this paper proposes the Relation-aware Entity Matching method,which embeds attribute relations into the original entity description to form sentences,so that entity matching is transformed into a sentence-level similarity determination task,based on Sentence-BERT completes sentence similarity calculation.We have conducted experiments on structured,dirty,and textual data,and compared them with baselines in recent years.Experimental results show that the use of relational embedding is helpful for entity matching on structured and dirty data.Our method has good results on most data sets for entity matching and reduces repeated calculations.

Non-stationary Buffeting Response Analysis of Long Span Suspension Bridge Under Strong Wind Loading

The non-stationary buffeting response of long span suspension bridge in time domain under strong wind loading is computed. Modeling method for generating non-stationary fluctuating winds with probabilistic model for non-stationary strong wind fields is first presented. Non-stationary wind forces induced by strong winds on bridge deck and tower are then given a brief introduction. Finally,Non-stationary buffeting response of Pulite Bridge in China,a long span suspension bridge,is computed by using ANSYS software under four working conditions with different combination of time-varying mean wind and time-varying variance. The case study further confirms that it is necessity of considering non-stationary buffeting response for long span suspension bridge under strong wind loading,rather than only stationary buffeting response.

Anticancer Effect of Plant-Derived Polysaccharides on Mice

Tupistra chinensis is a widely used medicinal plant in Hubei Province,China. In our study, we investigated the effect of polysaccharides component from Tupistra chinensis on mice in vivo. The mice H22 hepatocarcinoma animal model was established firstly. Then, the mice were fed with different doses of polysaccharides derived from Tupistra chinensis (one time each day). The neoplasm growth condition was analyzed and histological and electron microscopic analyses were performed. Compared to the control, Tupistra chinensis significantly inhibited the neoplasm growth. Large mass necrosis in the mouse model was observed under the light microscope and the necrosis area was significantly increased in the Tupistra chinensis-treated mice. Electron microscopic observations showed that severe apoptosis occurred in the cancer tissue in the mouse model and apoptosis was also significantly increased in Tupistra chinensis-treated mice. Our study demonstrated that Tupistra chinensis could inhibit the growth of H22 hepatocarcinoma. The mechanisms could involve the inhibitory effects of Tupistra chinensis on apoptosis, although the precise mechanisms remained to be elucidated in future study.

Risk factors and clinical outcomes of incomplete endoscopic resection of small rectal neuroendocrine tumors in southern China:a 9-year data analysis

Background:The histologically complete resection(CR)rate of small rectal neuroendocrine tumors(RNETs)is unsatisfactory at the first endoscopy.Risk factors and clinical outcomes associated with incomplete resection(IR)have not been explicitly elucidated.This study aims to explore the relevant factors of IR.Methods:This retrospective study reviewed patients with small RNETs(10mm)in eight centers from January 2013 to December 2021.Clinicopathological characteristics and clinical outcomes were compared between the CR and IR groups,and the polypectomy and advanced treatment groups.Results:Of the 326 patients included,83(25.5%)were diagnosed with IR.Polypectomy(odds ratio[OR]=16.86),a central depression(OR=7.50),and treatment in the early period(OR=2.60)were closely associated with IR.Further analysis revealed that an atypical hyperemic appearance(OR=7.49)and treatment in the early period(OR=2.54)were significantly associated with the inappropriate use of polypectomy(both P<0.05).In addition,a total of 265(81.3%)were followed up with a median follow-up period of 30.9 months.No death,metastasis,or recurrence was found during the follow-up period.Conclusions:Polypectomy,a central depression,and treatment in the early period were risk factors for IR.Further,an atypical hyperemic appearance and treatment in the early period were significant predisposing factors for inappropriate choice of polypectomy.For histologically incompletely resected small RNETs,follow-up may be a safe and feasible alternative to rigorous salvage therapy.

Variation of spatio-temporal distribution of on-road vehicle emissions based on real-time RFID data

High-resolution vehicular emissions inventories are important for managing vehicular pollution and improving urban air quality. This study developed a vehicular emission inventory with high spatio-temporal resolution in the main urban area of Chongqing, based on realtime traffic data from 820 RFID detectors covering 454 roads, and the differences in spatiotemporal emission characteristics between inner and outer districts were analysed. The result showed that the daily vehicular emission intensities of CO, hydrocarbons, PM2.5, PM10,and NO_(x) were 30.24, 3.83, 0.18, 0.20, and 8.65 kg/km per day, respectively, in the study area during 2018. The pollutants emission intensities in inner district were higher than those in outer district. Light passenger cars(LPCs) were the main contributors of all-day CO emissions in the inner and outer districts, from which the contributors of NO_(x) emissions were different. Diesel and natural gas buses were major contributors of daytime NO_(x) emissions in inner districts, accounting for 40.40%, but buses and heavy duty trucks(HDTs) were major contributors in outer districts. At nighttime, due to the lifting of truck restrictions and suspension of buses, HDTs become the main NO_(x) contributor in both inner and outer districts,and its three NO_(x) emission peak hours were found, which are different to the peak hours of total NO_(x) emission by all vehicles. Unlike most other cities, bridges and connecting channels are always emission hotspots due to long-time traffic congestion. This knowledge will help fully understand vehicular emissions characteristics and is useful for policymakers to design precise prevention and control measures.

自然杀伤性T细胞(NKT细胞)的LIGHT通过减少保护性的肺泡巨噬细胞加剧严重流感性肺炎

CD1d限制的NKT细胞是一类固有免疫样T淋巴细胞,在流感引起的肺炎中可以起到保护或阻碍的作用.本研究发现浸润到肺并激活的NKT细胞是LIGHT/TNFSF14的主要细胞来源,LIGHT加重了高致病性流感病毒感染(IAV)造成的肺部炎症.与野生型小鼠相比,LIGHT^(-/-)小鼠由于减少了肺泡巨噬细胞(AM)的凋亡从而减轻了肺损伤,因此对IAV的发病率和死亡率要显著降低.LIGHT优先促进LTβR+,而不是HVEM+的AM死亡.这些结果表明,NKT来源的LIGHT增加了组织保护性AM的细胞死亡,从而加重了肺部病理和对高致病性流感病毒的易感性.封闭LIGHT信号可能是治疗流感相关急性呼吸窘迫综合征(ARDS)可行的选择.

ARTICLE OPEN Viral dynamics and antibody responses in people with asymptomatic SARS-CoV-2 infection

Over 40% of the coronavirus disease 2019(COVID-19)COVID-19 patients were asymptomatically infected with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and the immune responses of these asymptomatic individuals is a critical factor for developing the strategy to contain the COVID-19 pandemic.Here,we determined the viral dynamics and antibody responses among 143 asymptomatic individuals identified in a massive screening of more than 5 million people in eight districts of Wuhan in May 2020.Asymptomatic individuals were admitted to the government-designated centralized sites in accordance with policy.The incidence rate of asymptomatic infection is〜2.92/100,000.These individuals had low viral copy numbers(peaked at 315 copies/mL)and short-lived antibody responses with the estimated diminish time of 69 days.The antibody responses in individuals with persistent SARS-CoV-2 infection is much longer with the estimated diminish time of 257 days.These results imply that the immune responses in the asymptomatic individuals are not potent enough for preventing SARS-CoV-2 re-infection,which has recently been reported in recovered COVID-19 patients.This casts doubt on the efficacy of forming"herd-immunity"through natural SARS-CoV-2 infection and urges for the development of safe and effective vaccines.