Zinc alleviates the heat stress of primary cultured hepatocytes of broiler embryos via enhancing the antioxidant ability and attenuating the heat shock responses

Zinc(Zn)has been shown to attenuate the adverse effects of heat stress on broilers,but the mechanisms involving this process remain unclear.We aimed to investigate possible protective mechanisms of Zn on primary cultured hepatocytes of broiler embryos subjected to heat stress.Three experiments were conducted.In Exp.1,hepatocytes were treated with 0,50,100,200,or 400μmol/L added Zn as inorganic Zn sulfate(iZn)for 12,24 or 48 h.In Exp.2,cells were exposed to 40℃(a normal temperature[NT])and 44℃(a high temperature[HT])for 1,2,4,6,or 8 h.In Exp.3,cells were preincubated with 0 or 50μmol/L Zn as iZn or organic Zn lysine chelate(oZn)for 8 h under NT,and then incubated with the same Zn treatments under NT or HT for 4 or 6 h.The biomarkers of antioxidative status and heat stress in cells were measured.The results in Exp.1 indicated that 50μmol/L Zn and 12 h incubation were the optimal conditions for increasing antioxidant ability of hepatocytes.In Exp.2,the 4 or 6 h incubation under HT was effective in inducing heat shock responses of hepatocytes.In Exp.3,HT elevated(P<0.01)malondialdehyde content and expressions of heat shock protein 70(HSP70)mRNA and protein,as well as HSP90 mRNA.However,Zn supplementation increased(P<0.05)copper zinc superoxide dismutase(CuZnSOD)activity and metallothionein mRNA expression,and effectively decreased(P<0.05)the ex-pressions of HSP70 mRNA and protein,as well as HSP90 mRNA.Furthermore,oZn was more effective(P<0.05)than iZn in enhancing CuZnSOD activity of hepatocytes under HT.It was concluded that Zn(especially oZn)could alleviate heat stress of broiler hepatocytes via enhancing their antioxidant ability and attenuating heat shock responses.

Influence of Mechanical Stratigraphy on the Deformation Evolution of Fold-Thrust Belts: Insights from the Analogue Modeling of Eastern Sichuan-Western Hunan and Hubei, South China

The eastern Sichuan-western Hunan and Hubei belt(ESWHHB) is an important fold-thrust belt in the Middle–Upper Yangtze region of China, and it is also an important area for petroleum and gas prospect in China. The influence of mechanical stratigraphy on the deformation evolution of the ESWHHB is a hot problem that has received widespread attention. However, due to the complexity of geological conditions, this issue has not been sufficiently addressed. Previews geological exploration studies show that the deformation evolution of the belt is closely related to the mechanical stratigraphy. Physical simulation has proven to be effective for studying the deformation evolution of fold-and-thrust belt. Based on the geological conditions of the ESWHHB, six groups of physical models were designed to analyze the influences of the ductile layer and overlap configuration on the structural deformation of the ESWHHB. The results show that the mechanical stratigraphy has significant control on the deformation evolution of the fold-thrust belt. The ESWHHB evolution is related to the lower viscosity of the ductile layer and the larger thickness of the ductile layer, while only gradual propagated fold-and-thrust belt can be resulted from the higher viscosity of the ductile layer and the smaller thickness of the ductile layer. Additionally, the overlap between the stratigraphy at various structural belts leads to significant differences in their mechanical properties, and it critically influences the structural patterns of the ESWHHB.

Reservoirs Patterns and Key Controlling Factors of the Lenghu Oil & Gas Field in the Qaidam Basin, Northwestern China

The Lenghu area is one of the most crucial tectonic belts for oil and gas exploration in the Qaidam Basin.However,reservoir distribution and the factors controlling petroleum accumulation in this area have not been studied in detail.In this paper,the structural characteristics and controlling factors of oil and gas accumulation in the Lenghu belt are investigated based on seismic profiles combined with drilling logs and microphotographs data.Results indicate that the Lenghu belt has thick source rocks and has preserved hydrocarbon generation conditions.Moreover,structural characteristics and lithology are the key factors controlling the reservoir distribution.The production of oil and gas layers are mainly distributed in high structural points and weak stress zones.The fracture zones and the weak stress zones are the potential places for the distribution of oil and gas reservoirs.