期刊文献+
共找到12篇文章
< 1 >
每页显示 20 50 100
MiR-221-3p调控c-Fos/beclin-1对大鼠冠状动脉微栓塞后心肌细胞自噬的影响 被引量:1
1
作者 吴文豪 李浪 +4 位作者 郑静 龙曼云 王现涛 孙羽涵 苏波 《广西医科大学学报》 CAS 2020年第4期559-568,共10页
目的:探究miR-221-3p介导的c-Fos/beclin-1信号通路在大鼠冠状动脉微栓塞(CME)后对心肌细胞自噬的影响及意义。方法:将24只SD大鼠按随机数字表法分为Sham组(假手术组)、CME组(微栓塞组)、CME+shRNA组(CME+AAV9-miR-221-3p shRNA组)和CME... 目的:探究miR-221-3p介导的c-Fos/beclin-1信号通路在大鼠冠状动脉微栓塞(CME)后对心肌细胞自噬的影响及意义。方法:将24只SD大鼠按随机数字表法分为Sham组(假手术组)、CME组(微栓塞组)、CME+shRNA组(CME+AAV9-miR-221-3p shRNA组)和CME+NC组(CME+AAV9-Control shRNA组),每组6只。经左心室注入微栓塞球建立CME模型,Sham组用等量生理盐水代替。各组术后9 h行心脏超声及心肌肌钙蛋白(IcTn-I)检测;组织取材做苏木精碱性复红苦味酸染色(HBFP)、透射电镜(TEM)、免疫荧光以及实时荧光定量聚合酶链反应(PCR)、蛋白质印迹(Western blot)等评估miR-221-3p介导的c-Fos/beclin-1信号通路在功能、形态和分子方面对CME的影响。结果:CME后心肌细胞中miR-221-3p表达升高,与Sham组相比,心脏功能抑制,cTn-I及心肌微梗死面积明显升高,c-Fos、beclin-1以及自噬指标LC3-Ⅱ蛋白表达降低(P<0.05)。下调miR-221-3p后,与CME组相比,cTn-I及心肌梗死面积显著下降(P<0.05),c-Fos、beclin-1以及LC3-Ⅱ蛋白表达增加(P<0.05),TEM提示自噬泡的双层膜结构数量增加,自噬部分恢复,心肌损伤减轻,心脏功能改善。结论:抑制miR-221-3p可改善大鼠CME后心脏功能,其可能通过c-Fos/beclin-1途径调节心肌自噬参与CME后诱导的心肌损伤。 展开更多
关键词 冠状动脉微栓塞 miR-221-3p C-FOS BECLIN-1 自噬
下载PDF
The first dinosaur tracksite from Xinjiang, NW China (Middle Jurassic Sanjianfang Formation, Turpan Basin)──a preliminary report 被引量:8
2
作者 Oliver WINCS Rico SCHELLHORN +3 位作者 Heinrich MALLISON Ben THUY wenhao wu Ge SUN 《Global Geology》 2007年第2期113-129,共17页
A new dinosaur tracksite was discovered in a steeply inclined sandstone layer of the Middle Jurassic Sanjianfang Formation in the Shanshan area of the Turpan Basin. The site is the first record of dinosaur footprints ... A new dinosaur tracksite was discovered in a steeply inclined sandstone layer of the Middle Jurassic Sanjianfang Formation in the Shanshan area of the Turpan Basin. The site is the first record of dinosaur footprints from Xinjiang Province in northwestern China. More than 150 tridactyl theropod dinosaur footprints are preserved as positive hyporeliefs on the lower bedding plane of a fine-grained sandstone body. Most of the footprints are isolated and appear to be randomly distributed. Some show well defined phalangeal pads, heels and rarely indistinct impressions of the distal part of the metatarsus. Two distinct morphotypes are present: a larger type with relatively broad pads shows similarities to Changpeipus and Megalosauripus, and a slightly smaller, slender and gracile type which is similar to Grallator, Eubrontes and Anchisauripus. In both morphotypes, digit III is the longest with a length between 11.4 and 33.6 cm. A single imprint shows prominent scratches, probably formed during slipping of the track maker. 展开更多
关键词 兽足亚目 恐龙 足迹 侏罗纪
下载PDF
Solid Model Bridge Static Damage Monitoring Based on GBSAR 被引量:2
3
作者 Sichun LONG Xiaoqin YUAN +4 位作者 Shide LU Wenting LIU Jinyu MA wenhao wu Chuanguang ZHU 《Journal of Geodesy and Geoinformation Science》 2022年第4期38-49,共12页
Bridge deformation monitoring usually adopts contact sensors,and the implementation process is often limited by the environment and observation conditions,resulting in unsatisfactory monitoring accuracy and effect.Gro... Bridge deformation monitoring usually adopts contact sensors,and the implementation process is often limited by the environment and observation conditions,resulting in unsatisfactory monitoring accuracy and effect.Ground-Based Synthetic Aperture Radar(GBSAR)combined with corner reflectors was used to perform static load-loaded deformation destruction experiments on solid model bridges in a non-contact manner.The semi parametric spline filtering and its optimization method were used to obtain the monitoring results of the GBSAR radar’s line of sight deformation,and the relative position of the corner reflector and the millimeter level deformation signals under different loading conditions were successfully extracted.The deformation transformation model from the radar line of sight direction to the vertical vibration direction was deduced.The transformation results of deformation monitoring and the measurement data such as the dial indicator were compared and analyzed.The occurrence and development process of bridge deformation and failure were successfully monitored,and the deformation characteristics of the bridge from continuous loading to eccentric loading until bridge failure were obtained.The experimental results show that GBSAR combined with corner reflector can be used for deformation feature acquisition,damage identification and health monitoring of bridges and other structures,and can provide a useful reference for design,construction and safety evaluation. 展开更多
关键词 GBSAR BRIDGE angular reflector static load test deformation monitoring
下载PDF
液态/准固态界面抑制锂硫电池中多硫化物在高极性电解质中的穿梭 被引量:1
4
作者 郭志杰 宋晓胜 +6 位作者 王晓兵 王晨晓 安旭慧 刘肖 吴文豪 卞腾飞 赵勇 《Science China Materials》 SCIE EI CAS CSCD 2023年第2期505-512,共8页
“穿梭效应”是锂硫电池基础研发和实际应用中最主要的挑战之一,特别是在富含多硫化物的高极性电解质中.本文展示了一项抑制穿梭效应的概念性研究,通过构建一个由高极性准固态电解质和弱极性液体电解质组成的液态/准固态两相界面来解决... “穿梭效应”是锂硫电池基础研发和实际应用中最主要的挑战之一,特别是在富含多硫化物的高极性电解质中.本文展示了一项抑制穿梭效应的概念性研究,通过构建一个由高极性准固态电解质和弱极性液体电解质组成的液态/准固态两相界面来解决该问题.弱极性电解质对多硫化物具有不溶解性,并且与锂金属具有良好的相容性,使锂对称电池的循环稳定性显著提升1400小时以上.此外,富含多硫化物的液态/准固态电解质的锂硫电池可实现超过300次的循环,表明液态/准固态电解质能有效抑制电解质组分与金属锂的副反应.液态/准固态两相电解质的概念为高极性溶剂在能源转换器件中的应用提供了一种新的可能性. 展开更多
关键词 锂硫电池 液体电解质 准固态电解质 穿梭效应 多硫化物 高极性 弱极性 极性溶剂
原文传递
A New EMG Decomposition Framework for Upper Limb Prosthetic Systems
5
作者 wenhao wu Li Jiang +3 位作者 Bangchu Yang Kening Gong Chunhao Peng Tianbao He 《Journal of Bionic Engineering》 SCIE EI CSCD 2023年第6期2646-2660,共15页
Neural interfaces based on surface Electromyography(EMG)decomposition have been widely used in upper limb prosthetic systems.In the current EMG decomposition framework,most Blind Source Separation(BSS)algorithms requi... Neural interfaces based on surface Electromyography(EMG)decomposition have been widely used in upper limb prosthetic systems.In the current EMG decomposition framework,most Blind Source Separation(BSS)algorithms require EMG with a large number of channels(generally larger than 64)as input,while users of prosthetic limbs can generally only provide less skin surface for electrode placement than healthy people.We performed decomposition tests to demonstrate the performance of the new framework with the simulated EMG signal.The results show that the new framework identified more Motor Units(MUs)compared to the control group and it is suitable for decomposing EMG signals with low channel numbers.In order to verify the application value of the new framework in the upper limb prosthesis system,we tested its performance in decomposing experimental EMG signals in force fitting experiments as well as pattern recognition experiments.The average Pearson coefficient between the fitted finger forces and the ground truth forces is 0.9079 and the average accuracy of gesture classification is 95.11%.The results show that the decomposition results obtained by the new framework can be used in the control of the upper limb prosthesis while only requiring EMG signals with fewer channels. 展开更多
关键词 EMG decomposition Peel-off strategy Few channels Upper limb prosthetic system
原文传递
单原子催化剂合成方法 被引量:4
6
作者 吴文浩 雷文 +2 位作者 王丽琼 王森 张海军 《化学进展》 SCIE CAS CSCD 北大核心 2020年第1期23-32,共10页
单原子催化剂作为一种原子尺度的催化剂,在制氢、CO氧化及光催化等领域均具有广阔的应用前景。大量实验结果和理论计算证实了金属单原子和载体之间的相互作用,及由两者之间电荷转移引起的电子结构改变是单原子催化剂具有高的选择性和催... 单原子催化剂作为一种原子尺度的催化剂,在制氢、CO氧化及光催化等领域均具有广阔的应用前景。大量实验结果和理论计算证实了金属单原子和载体之间的相互作用,及由两者之间电荷转移引起的电子结构改变是单原子催化剂具有高的选择性和催化活性的主要原因。本文着重综述了近年来共沉淀法、化学还原法及浸渍法所制备单原子催化剂的催化性能,并进行展望。 展开更多
关键词 单原子催化剂 共沉淀法 化学还原法 浸渍法
原文传递
Flabellariopteris, a new aquatic fern leaf from the Late Triassic of western Liaoning, China 被引量:2
7
作者 Chunlin Sun Tao Li +4 位作者 Yuling Na wenhao wu Yunfeng Li Lixia Wang Lijun Zhang 《Chinese Science Bulletin》 SCIE EI CAS 2014年第20期2410-2418,共9页
An aquatic fern leaf,Flabellariopteris mii Sun gen.et sp.nov.from the Late Triassic of Western Liaoning,China,is described and proposed as the type species of the new morphortaxon of the Marsileaceae.The fossil leaves... An aquatic fern leaf,Flabellariopteris mii Sun gen.et sp.nov.from the Late Triassic of Western Liaoning,China,is described and proposed as the type species of the new morphortaxon of the Marsileaceae.The fossil leaves are preserved as impression.No fertile and rhizomatic materials are found.The leaves attached at a common point on the top of the petiole are composed of two leaflets joined basally in an opposite arrangement.The petiole is slender.The leaflet is fan-shaped or semi-circular,and commonly divided into four wedge-shaped lobes.Terminal margin of lobes is usually incised,incisions shallow or deep,with bluntly rounded or notched apex.The veins are conspicuously dichotomous,but anastomoses and marginal vein are absent.The unique morphological features,including twoparted thin leaflets,size,shape,veins and slender petiole,indicate that the present specimen represents an aquatic fern,which is the oldest fossil record and the first megafossil evidence assignable to Marsileaceae from the Triassic floras. 展开更多
关键词 蕨类植物 植物叶子 晚三叠世 水生 中国 辽西 化石记录 信息产业部
原文传递
Diffuse Intrinsic Pontine Gliomas Exhibit Cell Biological and Molecular Signatures of Fetal Hindbrain-Derived Neural Progenitor Cells 被引量:2
8
作者 Yu Sun Cheng Xu +11 位作者 Changcun Pan Xin Chen Yibo Geng Yuliang wu Peng Zhang wenhao wu Yu Wang Deling Li Zhen wu Junting Zhang Qiaoran Xi Liwei Zhang 《Neuroscience Bulletin》 SCIE CAS CSCD 2019年第2期216-224,共9页
Diffuse intrinsic pontine glioma(DIPG) is the main cause of brain tumor-related death among children.Until now, there is still a lack of effective therapy with prolonged overall survival for this disease. A typical st... Diffuse intrinsic pontine glioma(DIPG) is the main cause of brain tumor-related death among children.Until now, there is still a lack of effective therapy with prolonged overall survival for this disease. A typical strategy for preclinical cancer research is to find out the molecular differences between tumor tissue and para-tumor normal tissue, in order to identify potential therapeutic targets. Unfortunately, it is impossible to obtain normal tissue for DIPG because of the vital functions of the pons.Here we report the human fetal hindbrain-derived neural progenitor cells(pontine progenitor cells, PPCs) as normal control cells for DIPG. The PPCs not only harbored similar cell biological and molecular signatures as DIPG glioma stem cells, but also had the potential to be immortalized by the DIPG-specific mutation H3 K27 M in vitro. These findings provide researchers with a candidate normal control and a potential medicine carrier for preclinical research on DIPG. 展开更多
关键词 Diffuse intrinsic PONTINE GLIOMA Neural PROGENITOR cells IMMORTALIZATION H3K27M SENESCENCE
原文传递
弥散张量成像对于康复期COVID-19患者神经损害的初步研究
9
作者 牛姗姗 韩佳悦 +3 位作者 吴文浩 廖健伟 罗联美 张亚琴 《中华介入放射学电子杂志》 2022年第4期422-428,共7页
目的应用弥散张量成像(diffusion tensor imaging,DTI)评估康复期COVID-19患者可能存在的白质纤维结构的改变,探讨SARS-CoV2对中枢神经系统可能造成的潜在迟发性损害。方法回顾性分析27例康复期COVID-19患者(普通患者组17例,重症患者组1... 目的应用弥散张量成像(diffusion tensor imaging,DTI)评估康复期COVID-19患者可能存在的白质纤维结构的改变,探讨SARS-CoV2对中枢神经系统可能造成的潜在迟发性损害。方法回顾性分析27例康复期COVID-19患者(普通患者组17例,重症患者组10例)和39例健康对照组的临床及颅脑MRI影像学资料。结果重症患者组的炎症反应及凝血功能异常较普通患者组明显;DTI结果:重症患者组多个神经纤维束的平均扩散率(mean diffusivity,MD)增加(P<0.05,FDR校正),主要分布在小脑中脚、胼胝体体部、双侧前、上放射冠区、双侧内囊前肢、左侧皮质脊髓束、左侧外囊、左侧上额枕束、毯及右侧下额枕束、右侧勾束等;小脑中脚各向异性分数(fractional anisotropy,FA)值下降(P<0.05,FDR校正)。结论康复期COVID-19患者存在白质纤维结构的改变,SARS-CoV2可能对中枢神经系统造成持续性或迟发性损害。DTI可提供有效的神经系统病毒感染的证据,有助于疾病的早期诊断。 展开更多
关键词 DTI COVID-19 SARS-CoV2 康复期 中枢神经系统
原文传递
Sorption mechanism of naphthalene by diesel soot: Insight from displacement with phenanthrene/p-nitrophenol
10
作者 wenhao wu Yun Huang +1 位作者 Daohui Lin Kun Yang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2021年第8期136-146,共11页
The nonlinear sorption of hydrophobic organic contaminants(HOCs)could be changed to linear sorption by the suppression of coexisting solutes in natural system,resulting in the enhancement of mobility,bioavailability a... The nonlinear sorption of hydrophobic organic contaminants(HOCs)could be changed to linear sorption by the suppression of coexisting solutes in natural system,resulting in the enhancement of mobility,bioavailability and risks of HOCs in the environment.In previous study,inspired from the competitive adsorption on activated carbon(AC),the displaceable fraction of HOCs sorption to soot by competitor was attributed to the adsorption on elemental carbon fraction of soot(EC-Soot),while the linear and nondisplaceable fraction was attributed to the partition in authigenic organic matter of soot(OM-Soot).In this study,however,we observed that the linear and nondisplaceable fraction of HOC(naphthalene)to a diesel soot(D-Soot)by competitor(phenanthrene or p-nitrophenol)should be attributed to not only the linear partition in OM-Soot,but also the residual linear adsorption on EC-Soot.We also observed that the competition on the surface of soot dominated by external surface was different from that of AC dominated by micropore surface,i.e.,complete displacement of HOCs by p-nitrophenol could occur for the micropore surface of AC,but not for the external surface of soot.These observations were obtained through the separation of EC-Soot and OM-Soot from D-Soot with organic-solvent extraction and the sorption comparisons of D-Soot with an AC(ACF300)and a multiwalled carbon nanotube(MWCNT30).The obtained results would give new insights to the sorption mechanisms of HOCs by soot and help to assess their environmental risks. 展开更多
关键词 Competitive sorption Diesel soot Organic compounds LINEARITY External surface
原文传递
发达地区公共服务支出实现了供需匹配吗?——以广东省及其21个地级市为例
11
作者 冯剑锋 黄博函 吴文浩 《中国公共政策评论》 2020年第1期178-191,共14页
本文以广东省为例,利用2007—2016年全省时间序列和21个地级市面板数据就公共教育、医疗、社会保障与就业、社区治理和公共安全五个方面对广东地区的公共服务支出进行了深入研究。通过构建公共服务支出的供需匹配模型发现:(1)2007—2016... 本文以广东省为例,利用2007—2016年全省时间序列和21个地级市面板数据就公共教育、医疗、社会保障与就业、社区治理和公共安全五个方面对广东地区的公共服务支出进行了深入研究。通过构建公共服务支出的供需匹配模型发现:(1)2007—2016年,广东公共服务支出并没有达到供需匹配状态,其中公共安全和社会管理支出出现严重不足,医疗卫生出现较为不足,公共教育和社会保障及就业支出则出现略为不足;(2)从各区域分布来看,公共服务支出水平在各地均有一定程度的供给不足现象,需要针对各地常住人口规模进行有效协调。公共服务支出的供需拥挤程度与支出水平和常住人口规模存在一定关联,应针对不同公共服务项目进行合理规划,加强科学评估和完善相关制度,逐步实现公共服务支出水平与常住人口规模相匹配的社会发展目标。 展开更多
关键词 基本公共服务 人口规模 财政支出 匹配研究
原文传递
Topological defects strengthened nonradical oxidation performance of biochar catalyzed peroxydisulfate system
12
作者 Xiaozeng Miao Xiliang Chen +2 位作者 wenhao wu Daohui Lin Kun Yang 《Biochar》 SCIE CAS 2023年第1期695-709,共15页
Nonradical oxidation based on peroxydisulfate(PDS)activation has attracted increasing attention for selective degradation of organic pollutants.Herein,topological defects were introduced into biochar(BC)via removing N... Nonradical oxidation based on peroxydisulfate(PDS)activation has attracted increasing attention for selective degradation of organic pollutants.Herein,topological defects were introduced into biochar(BC)via removing N atoms in N-doped BC(NBC)in an attempt to improve the nonradical catalytic performance.Compared to the pristine BC and NBC,the introduction of topological defects could achieve up to 36.6-and 8.7-times catalytic activity enhancement,respectively.More importantly,it was found that the catalytic activity was dominated by topological defects,which was verified by the significant positive correlation between the pseudo-first-order rate constants and the content of topological defects.Theoretical calculations suggested that topological defects enhanced the electrondonating ability of BC by reducing the energy gap,which made the electrons transfer to PDS molecules more easily.As a result,holes were generated after the carbon defects lost electrons,and induced a nonradical oxidation process.Benefiting from the merits of nonradical oxidation,the developed BC/PDS system showed superior performance in removing electron-rich contaminants in the presence of inorganic anions and in the actual environments.This study not only provides a potential avenue for designing efficient biochar-based catalysts,but also advances the mechanism understanding of nonradical oxidation process induced by carbon defects. 展开更多
关键词 Nonradical oxidation Peroxydisulfate Metal-free biochar Topological defects Electron transfer
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部