The acquisition of images with a fish-eye lens can cause serious image distortion because of the short focal length of the lens.As a result,it is difficult to use the obtained image information.To make use of the effe...The acquisition of images with a fish-eye lens can cause serious image distortion because of the short focal length of the lens.As a result,it is difficult to use the obtained image information.To make use of the effective information in the image,these distorted imagesmust first be corrected into the perspective of projection images in accordance with the human eye’s observation abilities.To solve this problem,this study presents an adaptive classification fitting method for fish-eye image correction.The degree of distortion in the image is represented by the difference value of the distances fromthe distorted point and undistorted point to the center of the image.The target points selected in the image are classified by the difference value.In the areas classified by different distortion differences,different parameter curves were used for fitting and correction.The algorithm was verified through experiments.The results showed that this method has a substantial correction effect on fish-eye images taken by different fish-eye lenses.展开更多
GTPase-activating SH3 domain-binding protein 2(G3BP2)is a mediator that responds to environmental stresses through stress granule formation and is involved in the progression of chronic diseases.However,no studies hav...GTPase-activating SH3 domain-binding protein 2(G3BP2)is a mediator that responds to environmental stresses through stress granule formation and is involved in the progression of chronic diseases.However,no studies have examined the contribution of G3BP2 in the oscillatory shear stress(OSS)-induced endothelial dysfunction.Here we assessed the effects of G3BP2 in endothelial cells(ECs)function and investigated the underlying mechanism.Using shear stress apparatus and partial ligation model,we identified that stress granulerelated genes in ECs could be induced by OSS with RNA-seq,and then confirmed that G3BP2 was highly and specifically expressed in athero-susceptible endothelia in the OSS regions.G3bp2e/eApoee/e mice had significantly decreased atherosclerotic lesions associated with deficiency of G3BP2 in protecting endothelial barrier function,decreasing monocyte adhesion to ECs and inhibiting the proinflammatory cytokine levels.Furthermore,loss of G3BP2 diminished OSS-induced inflammation in ECs by increasing YAP nucleocytoplasmic shuttling and phosphorylation.These data demonstrate that G3BP2 is a critical OSS regulated gene in regulating ECs function and that G3BP2 inhibition in ECs is a promising atheroprotective therapeutic strategy.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.51775390)the Open Research Fund Program of Hubei Provincial Key Laboratory of Chemical Equipment Intensification and Intrinsic Safety(Grant Nos.2016KA02 and 2018KA01).
文摘The acquisition of images with a fish-eye lens can cause serious image distortion because of the short focal length of the lens.As a result,it is difficult to use the obtained image information.To make use of the effective information in the image,these distorted imagesmust first be corrected into the perspective of projection images in accordance with the human eye’s observation abilities.To solve this problem,this study presents an adaptive classification fitting method for fish-eye image correction.The degree of distortion in the image is represented by the difference value of the distances fromthe distorted point and undistorted point to the center of the image.The target points selected in the image are classified by the difference value.In the areas classified by different distortion differences,different parameter curves were used for fitting and correction.The algorithm was verified through experiments.The results showed that this method has a substantial correction effect on fish-eye images taken by different fish-eye lenses.
基金This work was supported by the National Natural Science FoundationofChina(No.31971242 and12032007 toG.W.)The Natural Science Foundation of Chongqing,China(No.cstc2019jcyj-zdxmX0028 to G.W.,cstc2019jcyj-xfkxX0004 to J.Q.)+2 种基金Open Fund of Tianjin Enterprise Key Laboratory on Hyaluronic Acid Application Research,China(No.KTRDHAY201903 to G.W.)The Fundamental Research Funds for the Central Universities,China(No.2019CDYGZD008 to J.Q.)Chongqing Municipal Education Commission,China(No.KYYJ202001 to G.W.).
文摘GTPase-activating SH3 domain-binding protein 2(G3BP2)is a mediator that responds to environmental stresses through stress granule formation and is involved in the progression of chronic diseases.However,no studies have examined the contribution of G3BP2 in the oscillatory shear stress(OSS)-induced endothelial dysfunction.Here we assessed the effects of G3BP2 in endothelial cells(ECs)function and investigated the underlying mechanism.Using shear stress apparatus and partial ligation model,we identified that stress granulerelated genes in ECs could be induced by OSS with RNA-seq,and then confirmed that G3BP2 was highly and specifically expressed in athero-susceptible endothelia in the OSS regions.G3bp2e/eApoee/e mice had significantly decreased atherosclerotic lesions associated with deficiency of G3BP2 in protecting endothelial barrier function,decreasing monocyte adhesion to ECs and inhibiting the proinflammatory cytokine levels.Furthermore,loss of G3BP2 diminished OSS-induced inflammation in ECs by increasing YAP nucleocytoplasmic shuttling and phosphorylation.These data demonstrate that G3BP2 is a critical OSS regulated gene in regulating ECs function and that G3BP2 inhibition in ECs is a promising atheroprotective therapeutic strategy.