Incorporation of Mg-phenolic networks as a protective coating for magnesium alloy to enhance corrosion resistance and osteogenesis in vivo

Magnesium(Mg) and its alloys have been intensively studied to develop the next generation of bone implants recently, but their clinical application is restricted by rapid degradation and unsatisfied osteogenic effect in vivo. A bioactive chemical conversion Mg-phenolic networks complex coating(e EGCG) was stepwise incorporated by epigallocatechin-3-gallate(EGCG) and exogenous Mg^(2+)on Mg-2Zn magnesium alloy. Simplex EGCG induced chemical conversion coating(c EGCG) was set as compare group. The in vitro corrosion behavior of Mg-2Zn alloy, c EGCG and e EGCG was evaluated in SBF using electrochemical(PDP, EIS) and immersion test. The cytocompatibility was investigated with rat bone marrow mesenchymal stem cells(r BMSCs). Furthermore, the in vivo tests using a rabbit model involved micro computed tomography(Micro-CT) analysis, histological observation, and interface analysis. The results showed that the e EGCG is Mgphenolic multilayer coating incorporated Mg-phenolic networks, which is rougher, more compact and much thicker than c EGCG. The e EGCG highly improved the corrosion resistance of Mg-2Zn alloy, combined with its lower average hemolytic ratios, continuous high scavenging effect ability and relatively moderate contact angle features, resulting in a stable and suitable biological environment, obviously promoted r BMSCs adhesion and proliferation. More importantly, Micro-CT, histological and interface elements distribution evaluations all revealed that the e EGCG effectively inhibited degradation and enhanced bone tissue formation of Mg alloy implants. This study puts forward a promising bioactive chemical conversion coating with Mg-phenolic networks for the application of biodegradable orthopedic implants.

Dynamic Characteristics of Metro Vehicle under Thermal Deformation of Long-Span Cable-Stayed Bridge

In order to study the influence of thermal deformation of long-span cable- stayed bridge (LSCSB) on the dynamic characteristics of metro vehicle on the bridge, based on the theory of vehicle-track coupled dynamics, the rigid-flexible coupled dynamic model of metro vehicle-track-LSCSB system is established by using finite element method and multi-rigid-body dynamics. Adopting this model, the deformation of LSCSB subject to temperature is analyzed, then the comprehensive effect of track random irregularity and rail deformation caused by temperature load is considered to study the dynamic characteristics of metro vehicle running through the bridge, and finally the influences of temperature increment and running speed on concerned dynamic indices of vehicle are studied. The results show that the LSCSB deforms obviously subject to temperature load, and the overall performance is that the cooling is arched, and the heating is bent, and the shape variable changes almost linearly with the temperature load. According to the parameters studied in this paper, the rail deformation caused by temperature load increases the wheel-rail vertical force, derailment coefficient and wheel load reduction rate by 1.5%, 3.1% and 5% respectively. The vertical acceleration of the vehicle body decreases by 2.4% under the cooling condition, while increases by 3.7% under the heating condition. The dynamic response of the bridge changes under temperature load. The maximum vertical and horizontal displacement in the middle of the main beam span are 6.24 mm and 2.19 mm respectively, and the maximum vertical and horizontal acceleration are 1.29 cm/s2 and 2.54cm/s2 respectively. The derailment coefficient and vertical acceleration of vehicle body are more affected by temperature load, and the wheel load reduction rate and wheel-rail vertical force are more affected by speed. The conclusion of this paper provides a reference for subsequent scholars to study the influence of thermal deformation on the dynamic response of vehicles on LSCSB.

5-氟尿嘧啶和卡培他滨对中国患者的心脏毒性:一项前瞻性研究

背景与目的已有许多研究报道5-氟尿嘧啶(5-Fluorouracil,5-FU)和卡培他滨相关的心脏毒性,严重程度从无症状心电图(electrocardiography,ECG)异常到严重心肌梗塞而不同。目前为止,对中国恶性肿瘤患者的这种心脏毒性尚无研究。本研究旨在前瞻性地对多个中心招募的癌症患者进行5-FU和卡培他滨相关心脏毒性的发生率和临床表现进行评估。方法在完成研究的527例患者中,196例接受基于5-FU的化疗,331例接受基于卡培他滨的化疗并以其为一线或辅助治疗。在治疗期间和长达28 d的随访期间对所发生的不良反应进行记录。疗效测量指标包括心电图、心肌酶、心肌肌钙蛋白、脑钠肽和超声心动图。使用单因素分析和logistic回归方法进行亚组分析和鉴定与两种药物的心脏毒性相关的显著性独立变量。结果 527例患者中有161例(30.6%)出现心脏毒性。卡培他滨组的心脏毒性发生率为33.8%(112/331),显著高于5-FU组25%(49/196)的发生率(P=0.0042)。110/527患者(20.9%)发生心律失常,105/527(19.9%)发生缺血性改变,而只有20/527患者(3.8%)出现心力衰竭,6/527患者(1.1%)出现心肌梗塞。既往的心脏病、高血压、卡培他滨为基础的化疗和治疗时间被确定为与心脏毒性相关的显著风险因素。优势比分别为15.7(心脏病史vs.无病史)、1.86(卡培他滨vs. 5-FU)、1.06(5–8个化疗周期vs. 1–4个化疗周期)和1.58(高血压vs.无高血压)。结论氟嘧啶类药物在中国人群中引起的心脏毒性可能在临床实践中被低估。建议密切监测患者,尤其是对心脏毒性高风险的患者。可能的风险因素包括治疗持续时间、基于卡培他滨的化疗、既往心脏病史和高血压史。

An Interactive Online Platform for Algorithms Teaching

Learning programming has become an important part of education.However,most students have extreme difficulty learning programming and complex algorithms.This is because programming has a hierarchical logic.Solving complex problems requires students to develop skills in decomposing problems.To this end,this paper describes an effective method to develop an online platform for teaching complex algorithms.

PICOTEES:a privacy-preserving online service of phenotype exploration for genetic-diagnostic variants from Chinese children cohorts

The growth in biomedical data resources has raised potential privacy concerns and risks of genetic information leakage. For instance, exome sequencing aids clinical decisions by comparing data through web services, but it requires significant trust between users and providers. To alleviate privacy concerns, the most commonly used strategy is to anonymize sensitive data. Unfortunately, studies have shown that anonymization is insufficient to protect against reidentification attacks. Recently, privacy-preserving technologies have been applied to preserve application utility while protecting the privacy of biomedical data. We present the PICOTEES framework, a privacy-preserving online service of phenotype exploration for genetic-diagnostic variants (https://birthdefectlab.cn:3000/). PICOTEES enables privacy-preserving queries of the phenotype spectrum for a single variant by utilizing trusted execution environment technology, which can protect the privacy of the user's query information, backend models, and data, as well as the final results. We demonstrate the utility and performance of PICOTEES by exploring a bioinformatics dataset. The dataset is from a cohort containing 20,909 genetic testing patients with 3,152,508 variants from the Children's Hospital of Fudan University in China, dominated by the Chinese Han population (>99.9%). Our query results yield a large number of unreported diagnostic variants and previously reported pathogenicity.

A Novel Rolling and Fractional-ordered Grey System Model and Its Application for Predicting Industrial Electricity Consumption

Accurate and reasonable prediction of industrial electricity consumption is of great significance for promoting regional green transformation and optimizing the energy structure.However,the regional power system is complicated and uncertain,affected by multiple factors including climate,population and economy.This paper incorporates structure expansion,parameter optimization and rolling mechanism into a system forecasting framework,and designs a novel rolling and fractional-ordered grey system model to forecast the industrial electricity consumption,improving the accuracy of the traditional grey models.The optimal fractional order is obtained by using the particle swarm optimization algorithm,which enhances the model adaptability.Then,the proposed model is employed to forecast and analyze the changing trend of industrial electricity consumption in Fujian province.Experimental results show that industrial electricity consumption in Fujian will maintain an upward growth and it is expected to 186.312 billion kWh in 2026.Compared with other seven benchmark prediction models,the proposed grey system model performs best in terms of both simulation and prediction performance metrics,providing scientific reference for regional energy planning and electricity market operation.

Magnesium surface-activated 3D printed porous PEEK scaffolds for in vivo osseointegration by promoting angiogenesis and osteogenesis

Polyetheretherketone(PEEK)has been an alternative material for titanium in bone defect repair,but its clinical application is limited by its poor osseointegration.In this study,a porous structural design and activated surface modification were used to enhance the osseointegration capacity of PEEK materials.Porous PEEK scaffolds were manufactured via fused deposition modeling and a polydopamine(PDA)coating chelated with magnesium ions(Mg^(2+))was utilized on the surface.After surface modification,the hydrophilicity of PEEK scaffolds was significantly enhanced,and bioactive Mg^(2+)could be released.In vitro results showed that the activated surface could promote cell proliferation and adhesion and contribute to osteoblast differentiation and mineralization;the released Mg^(2+)promoted angiogenesis and might contribute to the formation of osteogenic H-type vessels.Furthermore,porous PEEK scaffolds were implanted in rabbit femoral condyles for in vivo evaluation of osseointegration.The results showed that the customized three-dimensional porous structure facilitated vascular ingrowth and bone ingrowth within the PEEK scaffolds.The PDA coating enhanced the interfacial osseointegration of porous PEEK scaffolds and the released Mg^(2+)accelerated early bone ingrowth by promoting early angiogenesis during the coating degradation process.This study provides an efficient solution for enhancing the osseointegration of PEEK materials,which has high potential for translational clinical applications.

Genetic background of idiopathic neurodevelopmental delay patients with significant brain deviation volume

Background:Significant brain volume deviation is an essential phenotype in children with neurodevelopmental delay(NDD),but its genetic basis has not been fully characterized.This study attempted to analyze the genetic factors associated with significant whole-brain deviation volume(WBDV).Methods:We established a reference curve based on 4222 subjects ranging in age from the first postnatal day to 18 years.We recruited only NDD patients without acquired etiologies or positive genetic results.Cranial magnetic resonance imaging(MRI)and clinical exome sequencing(2742 genes)data were acquired.A genetic burden test was performed,and the results were compared between patients with and without significant WBDV.Literature review analyses and BrainSpan analysis based on the human brain developmental transcriptome were performed to detect the potential role of genetic risk factors in human brain development.Results:We recruited a total of 253 NDD patients.Among them,26 had significantly decreased WBDV(<-2 standard deviations[SDs]),and 14 had significantly increased WBDV(>+2 SDs).NDD patients with significant WBDV had higher rates of motor development delay(49.8%[106/213]vs.75.0%[30/40],P=0.003)than patients without significant WBDV.Genetic burden analyses found 30 genes with an increased allele frequency of rare variants in patients with significant WBDV.Analyses of the literature further demonstrated that these genes were not randomly identified:burden genes were more related to the brain development than background genes(P=1.656e^(-9)).In seven human brain regions related to motor development,we observed burden genes had higher expression before 37-week gestational age than postnatal stages.Functional analyses found that burden genes were enriched in embryonic brain development,with positive regulation of synaptic growth at the neuromuscular junction,positive regulation of deoxyribonucleic acid templated transcription,and response to hormone,and these genes were shown to be expressed in neural progenitors.Based on single cell sequencing analyses,we found TUBB2B gene had elevated expression levels in neural progenitor cells,interneuron,and excitatory neuron and SOX15 had high expression in interneuron and excitatory neuron.Conclusion:Idiopathic NDD patients with significant brain volume changes detected by MRI had an increased prevalence of motor development delay,which could be explained by the genetic differences characterized herein.

Biomimetic AgNPs@antimicrobial peptide/silk fibroin coating for infection-trigger antibacterial capability and enhanced osseointegration

Endowing implant surfaces with combined antibacterial and osteogenic properties by drug-loaded coatings has made great strides,but how to achieve the combined excellence of infection-triggered bactericidal and in vivo-proven osteogenic activities without causing bacterial resistance still remains a formidable challenge.Herein,antimicrobial peptides(AMPs)with osteogenic fragments were designed and complexed on the surface of silver nanoparticle(AgNP)through hydrogen bonding,and the collagen structure-bionic silk fibroin(SF)was applied to carry AgNPs@AMPs to achieve infection-triggered antibacterial and osteointegration.As verified by TEM,AMPs contributed to the dispersion and size-regulation of AgNPs,with a particle size of about 20 nm,and a clear protein corona structure was observed on the particle surface.The release curve of silver ion displayed that the SF-based coating owned sensitive pH-responsive properties.In the antibacterial test against S.aureus for up to 21 days,the antibacterial rate had always remained above 99%.Meanwhile,the underlying mechanism was revealed,originating from the destruction of the bacterial cell membranes and ROS generation.The SF-based coating was conducive to the adhesion,diffusion,and proliferation of bone marrow stem cells(BMSCs)on the surface,and promoted the expression of osteogenic genes and collagen secretion.The in vivo implantation results showed that compared with the untreated Ti implants,SF-based coating enhanced osseointegration at week 4 and 8.Overall,the AgNPs@AMPs-loaded SF-based coating presented the ability to synergistically inhibit bacteria and promote osseointegration,possessing tremendous potential application prospects in bone defects and related-infection treatments.

Microstructure Evolution of Heat‑Affected Zone in Submerged Arc Welding and Laser Hybrid Welding of 690 MPa High Strength Steel and its Relationship with Ductile–Brittle Transition Temperature

The comparative study of submerged arc welding(SAW)and laser hybrid welding(LHW)was carried out for a 690 MPa high strength steel with thickness of 20 mm.Microstructure and ductile–brittle transition temperature(DBTT)evolution in welded zone were elucidated from the aspect of crystallographic structure,particularly,digitization and visualization of 24 variants.The impact toughness of each micro zone in LHW joint is better than that of SAW,in which the DBTT of equivalent fusion line and heat-affected zone(HAZ)can reach−70 and−80℃,while that of SAW is only−50℃.LHW technology induces narrowing of the HAZ and refining of the microstructure obtained in weld metal and HAZ.Meanwhile,the austenite grain size and transformation driving force in the coarse grained heat-affected zone(CGHAZ)are reduced and increased,respectively.It makes variant selection mechanism occurring in CGHAZ of LHW dominate by close-packed plane grouping,which promotes lath bainite formation with high density of high angle grain boundary,especially block boundary dominated by V1/V2 pair.While for SAW,the lower transformation driving force inferred from the large amount of retained austenite in CGHAZ induces Bain grouping of variants,and thus triggers the brittle crack propagating straightly in granular bainite,resulting in lower impact toughness and higher DBTT.

Dual-sized hollow particle incorporated fibroin thermal insulating coatings on catheter for cerebral therapeutic hypothermia

Selective endovascular hypothermia has been used to provide cooling-induced cerebral neuroprotection,but current catheters do not support thermally-insulated transfer of cold infusate,which results in an increased exit temperature,causes hemodilution,and limits its cooling efficiency.Herein,air-sprayed fibroin/silica-based coatings combined with chemical vapor deposited parylene-C capping film was prepared on catheter.This coating features in dual-sized-hollow-microparticle incorporated structures with low thermal conductivity.The infusate exit temperature is tunable by adjusting the coating thickness and infusion rate.No peeling or cracking was observed on the coatings under bending and rotational scenarios in the vascular models.Its efficiency was verified in a swine model,and the outlet temperature of coated catheter(75μm thickness)was 1.8-2.0◦C lower than that of the uncoated one.This pioneering work on catheter thermal insulation coatings may facilitate the clinical translation of selective endovascular hypothermia for neuroprotection in patients with acute ischemic stroke.

Viral load kinetics of the severe acute respiratory syndrome coronavirus 2 Omicron variant in children aged 0 to 3 years and their parents

The Omicron variant of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was first confirmed in November 2021 in South Africa^([1])and is more transmissible than other sub-variants.^([2])The influence of Omicron in children has been recognized with increased hospital admission rate than Delta wave.^([3,4])But severe clinical outcomes and comorbidity of Omicron are less than Delta in children.^([5])Hospitalized children during the Omicron period were more likely to be younger than that in the pre-Omicron period.^([6])

Artificial womb:a paradigm shift for saving extremely premature infants

To the Editor:Approximately 15 million preterm babies,i.e.,those delivered at<37 weeks of gestational age(GA),are born globally every year,and of them,0.4%are extremely premature infants(EPIs),i.e.,those delivered at<28 weeks of GA.^([1])Iterations of neonatal care have significantly extended the viability of preterm babies.However,improved viability is accompanied by an increased risk of unreversed injuries,such as bronchopulmonary dysplasia and intraventricular hemorrhage.Therefore,a more physiological simulating in utero status is needed to protect EPIs’immature organs during the transitional period and ensure that they develop in the same manner that they would have in the womb.

Influence of heat treatment on microstructure,mechanical and corrosion behavior of WE43 alloy fabricated by laser-beam powder bed fusion

Magnesium(Mg)alloys are considered to be a new generation of revolutionary medical metals.Laser-beam powder bed fusion(PBF-LB)is suitable for fabricating metal implants withpersonalized and complicated structures.However,the as-built part usually exhibits undesirable microstructure and unsatisfactory performance.In this work,WE43 parts were firstly fabricated by PBF-LB and then subjected to heat treatment.Although a high densification rate of 99.91%was achieved using suitable processes,the as-built parts exhibited anisotropic and layeredmicrostructure with heterogeneously precipitated Nd-rich intermetallic.After heat treatment,fine and nano-scaled Mg24Y5particles were precipitated.Meanwhile,theα-Mg grainsunderwent recrystallization and turned coarsened slightly,which effectively weakened thetexture intensity and reduced the anisotropy.As a consequence,the yield strength and ultimate tensile strength were significantly improved to(250.2±3.5)MPa and(312±3.7)MPa,respectively,while the elongation was still maintained at a high level of 15.2%.Furthermore,the homogenized microstructure reduced the tendency of localized corrosion and favoredthe development of uniform passivation film.Thus,the degradation rate of WE43 parts was decreased by an order of magnitude.Besides,in-vitro cell experiments proved their favorable biocompatibility.

High‑Resolution and Multidimensional Phenotypes Can Complement Genomics Data to Diagnose Diseases in the Neonatal Population

Advances in genomic medicine have greatly improved our understanding of human diseases.However,phenome is not well understood.High-resolution and multidimensional phenotypes have shed light on the mechanisms underlying neonatal diseases in greater details and have the potential to optimize clinical strategies.In this review,we first highlight the value of analyzing traditional phenotypes using a data science approach in the neonatal population.We then discuss recent research on high-resolution,multidimensional,and structured phenotypes in neonatal critical diseases.Finally,we briefly introduce current technologies available for the analysis of multidimensional data and the value that can be provided by integrating these data into clinical practice.In summary,a time series of multidimensional phenome can improve our understanding of disease mechanisms and diagnostic decision-making,stratify patients,and provide clinicians with optimized strategies for therapeutic intervention;however,the available technologies for collecting multidimensional data and the best platform for connecting multiple modalities should be considered.

Biomedical rare-earth magnesium alloy:Current status and future prospects

Biomedical magnesium(Mg)alloys have garnered significant attention because of their unique biodegradability,favorable biocompatibility,and suitable mechanical properties.The incorporation of rare earth(RE)elements,with their distinct physical and chemical properties,has greatly contributed to enhancing the mechanical performance,degradation behavior,and biological performance of biomedical Mg alloys.Currently,a series of RE-Mg alloys are being designed and investigated for orthopedic implants and cardiovascular stents,achieving substantial and encouraging research progress.In this work,a comprehensive summary of the state-of-the-art in biomedical RE-Mg alloys is provided.The physiological effects and design standards of RE elements in biomedical Mg alloys are discussed.Particularly,the degradation behavior and mechanical properties,including their underlying action are studied in-depth.Furthermore,the preparation techniques and current application status of RE-Mg alloys are reviewed.Finally,we address the ongoing challenges and propose future prospects to guide the development of high-performance biomedical Mg-RE alloys.

Programmable droplet manipulation enabled by charged-surface pattern reconfiguration

Programmable droplet manipulation based on external stimulation is in high demand in various modern technologies.Despite notable progress,current manipulation strategies still suffer from a common drawback such as single control means of modulating the external stimulation input,which leads to huge challenges in sophisticated and large scale-up droplet handling.Herein,a unique patternreconfiguration-driven droplet manipulation method is developed on conductive/nonconductive pattern surfaces under charge deposition.Contactless charge deposition induces the“edge barrier”phenomenon at the boundaries of conductive/nonconductive patterns,analogous to an invisible and tunable wall guiding droplet behaviors.The edge barrier effect can be flexibly tuned by the nonconductive surface pattern.Thus,with charge deposition,surfaces are endowed with protean control functionality.The design of conductive/nonconductive patterns can effectively enable multifunction droplet manipulations,including track-guided sliding,sorting,merging,and mixing.Moreover,dynamical pattern reconfiguration drives programmable fluidics with sophisticated and large scale-up droplet handling capabilities in a low-cost and simple approach.

Construction of fluorescent hyperbranched polysiloxane-based clusteroluminogens with enhanced quantum yield and efficient cellular lighting

Owing to its designability and intrinsic fluorescence,non-conjugated hyperbranched polysiloxane(HBPSi)has attracted widespread attention in biological filed,while it is still severely restricted by low fluorescence efficiency.So,in this paper,we introduced disulfide into HBPSi improving their luminescence properties and synthesized different molecular weight HBPSi(P1,P2,and P3).Surprisingly,P1 exhibited ultrahigh quantum yield up to 47.81%.Meanwhile,experiments applied with theoretical calculations were employed to explore the fluorescence mechanism,which is attributed to efficient restricting of non-radiative decay by clusteroluminogens formed with the cooperation of hyperbranched structure and double hydrogen bonding.In addition,the biocompatibility of P1 was verified by co-culture with MC3T3-E1 and P1 lighted up mouse fibroblast cells without fluorescent dyes.This work designed a novel fluorescent polymer with ultrahigh fluorescence quantum yield and cell imaging ability,which is promising in visualization diagnosis and treatment of tumor.

影响Omicron变异株感染轻型和普通型COVID-19患儿核酸转阴时间缩短因素分析

目的探讨Omicron变异株感染轻型与普通型新型冠状病毒疾病(COVID-19)患儿的临床特征及影响其核酸检测结果转为阴性(以下简称为"核酸转阴")时间缩短因素。方法选取2022年4月7日至5月20日上海交通大学医学院附属仁济医院南院区"亲子病房"集中收治的150例Omicron变异株感染轻型和普通型COVID-19患儿为研究对象。采用回顾性分析方法,根据这150例患儿的核酸转阴中位时间为13 d,将其分别纳入快转阴组(n=84,核酸转阴时间<13 d)和慢转阴组(n=66,核酸转阴时间≥13 d)。对2组患儿年龄、性别、轻型与普通型COVID-19患儿构成比、疫苗接种率、COVID-19相关临床表现及COVID-19发病48 h内系统炎症指数(SII)、中性粒细胞与淋巴细胞比值(NLR)、淋巴细胞与单核细胞比值(LMR)、血小板与淋巴细胞比值(PLR)等,进行成组t检验、Mann-Whitney U检验或χ2检验。采用多因素非条件logistic回归分析方法,分析影响Omicron变异株感染轻型与普通型COVID-19患儿核酸转阴时间缩短的因素。2组患儿合并基础疾病占比、采取对症治疗率等一般临床资料分别比较,差异均无统计学意义(P>0.05)。本研究遵循的程序符合2013修订的《世界医学协会赫尔辛基宣言》要求。结果①快转阴组患儿年龄、女性患儿比例、普通型COVID-19患儿比例、疫苗接种率,发热、咽痛、胃肠道症状发生率,均显著高于慢转阴组,而热峰、肌肉酸痛发生率,则均显著低于慢转阴组,2组比较,差异均有统计学意义(P<0.05)。②多因素非条件logistic回归分析结果显示,伴有咽痛、接种疫苗是轻型与普通型COVID-19患儿核酸转阴时间缩短的独立保护因素(OR=14.609、7.866,95%CI:1.108~192.697、1.313~47.121,P=0.042、0.024),而伴有肌肉酸痛、男性患儿则是其独立危险因素(OR=0.275、0.206,95%CI:0.087~0.874、0.083~0.511,P=0.029、0.001)。③快转阴组患儿SII为250.9×109/L(111.1×109/L,466.9×109/L),NLR为1.0(0.6,2.2),均分别显著高于慢转阴组的109.8×109/L(47.2×109/L,266.7×109/L)与0.5(0.2,1.2),并且差异均有统计学意义(Z=6.48、5.34,P=0.011、0.021)。快转阴组患儿LMR为4.1(2.5,9.4),显著低于慢转阴组的8.2(3.4,11.1),并且差异有统计学意义(Z=4.50,P=0.034)。结论轻型与普通型COVID-19患儿临床表现不典型,以发热、咳嗽为主,高SII、NLR及低LMR患儿核酸转阴时间较短,接种疫苗、伴有咽痛是促进核酸转阴时间缩短的保护性因素,伴有肌肉酸痛及男性是影响核酸转阴时间缩短的危险因素。

聚多巴胺辅助微弧氧化后载肝素和万古霉素以提高3D打印多孔钛合金内植物的血液相容性和抗菌成骨功能（英文）

在研发多功能骨科生物材料时,抗菌能力、血液相容性、骨细胞相容性和骨整合性能是最重要的考虑因素.本文中,我们成功研发了具有良好血液相容性的抗菌、促成骨复合功能的3D打印多孔钛合金内植物.简言之,3D打印多孔钛合金支架经表面处理后具有多孔形貌的微弧氧化促成骨涂层,进而联结固定肝素钠和万古霉素.本文采用扫描电镜、X射线光电子能谱分析和傅里叶变换红外光谱学对其表面微观结构、形态和化学组成进行了表征,并用高效液相色谱法检测了其载药能力和万古霉素释放曲线.通过微生物检测、细菌死活染色和结晶紫染色,确定了其良好的抗金葡菌及菌膜性能.将人骨髓间充质干细胞在支架上培养后,用细胞计数试剂盒和细胞活性检测试剂盒检测了其增殖活性.此外,用碱性磷酸酶成活性作为标记检测了成骨细胞分化能力,通过检测活化部分凝血活酶时间、血浆凝血酶原时间和凝血酶时间,确定肝素化的支架血液相容性得到了提高.该研究结果表明,经过表面改性的3D打印多孔钛合金内植物拥有稳定的抗菌和促成骨功能,同时显现出抗凝特性.预示着其在未来能用于复杂骨缺损的定制化功能重建.