Shifting the Paradigm:A Fresh Look at Physical Activity Frequency and Its Impact on Mental Health,Life Satisfaction,and Self-Rated Health in Adolescents

As adolescent mental health problems are becoming a more serious issue globally,this paper explores the relationship of physical activity in adolescents and its frequency on mental health as well as examines the mediating effects of life satisfaction and self-rated health in order to provide a reference for the promotion of mental health in adolescents.A sample of 3578 Chinese high school students completed questionnaires assessing their mental health,physical activity frequency,life satisfaction,and self-rated health.The mean SCL-90 value for adolescents was found to be 1.629%,and 24.73%of adolescents had varying degrees of mental health issue.Increased physical activity frequency is positively associated with improved mental health(p<0.001).Additionally,life satisfaction and self-rated health were found to play significant mediating roles in the relationship between physical activity frequency and mental health.Specifically,low-frequency physical activity had the most pronounced mediating effect on mental health through life satisfaction,while high-frequency physical activity exerted the most significant mediating effect on mental health through self-rated health.These findings underscore the importance of promoting physical activity among adolescents and highlight the distinct pathways through which physical activity frequency can influence mental health outcomes.Further research is needed to explore these relationships in diverse populations and settings,as well as to develop targeted intervention.

Low-Carbon Dispatch of an Integrated Energy System Considering Confidence Intervals for Renewable Energy Generation

Addressing the insufficiency in down-regulation leeway within integrated energy systems stemming from the erratic and volatile nature of wind and solar renewable energy generation,this study focuses on formulating a coordinated strategy involving the carbon capture unit of the integrated energy system and the resources on the load storage side.A scheduling model is devised that takes into account the confidence interval associated with renewable energy generation,with the overarching goal of optimizing the system for low-carbon operation.To begin with,an in-depth analysis is conducted on the temporal energy-shifting attributes and the low-carbon modulation mechanisms exhibited by the source-side carbon capture power plant within the context of integrated and adaptable operational paradigms.Drawing from this analysis,a model is devised to represent the adjustable resources on the charge-storage side,predicated on the principles of electro-thermal coupling within the energy system.Subsequently,the dissimilarities in the confidence intervals of renewable energy generation are considered,leading to the proposition of a flexible upper threshold for the confidence interval.Building on this,a low-carbon dispatch model is established for the integrated energy system,factoring in the margin allowed by the adjustable resources.In the final phase,a simulation is performed on a regional electric heating integrated energy system.This simulation seeks to assess the impact of source-load-storage coordination on the system’s low-carbon operation across various scenarios of reduction margin reserves.The findings underscore that the proactive scheduling model incorporating confidence interval considerations for reduction margin reserves effectively mitigates the uncertainties tied to renewable energy generation.Through harmonized orchestration of source,load,and storage elements,it expands the utilization scope for renewable energy,safeguards the economic efficiency of system operations under low-carbon emission conditions,and empirically validates the soundness and efficacy of the proposed approach.

Viability of primary cultured retinal neurons in a hyperglycemic condition

The retina of Wistar rats within 1-3 days of birth were dissociated into a retinal ceil suspension using 0.05% trypsin digestion. The cell suspension was incubated in Dulbecco＇s modified Eagle＇s medium for 24 hours, followed by neurobasal medium for 5-7 days. Nissl staining showed that 79.86% of primary cultured retinal cells were positive and immunocytochemical staining showed that the purity of anti-neurofilament heavy chain antibody-positive cells was 71.53%, indicating that the primary culture system of rat retinal neurons was a reliable and stable cell system with neurons as the predominant cell type. The primary cultured retinal neurons were further treated with 0, 5.5, 15, 25, and 35 mM glucose for 24, 48, and 72 hours. The thiazolyl blue tetrazolium bromide test and flow cytometry showed that with increasing glucose concentration and treatment duration, the viability of retinal neurons was reduced, and apoptosis increased. In particular, 35 mM glucose exhibited the most significant effect at 72 hours. Thus, rat retinal neurons treated with 35 mM glucose for 72 hours can be used to simulate a neuronal model of diabetic retinopathy.

The Current Situation of Quality Supervision and Production Safety of Aquatic Products in Nanjing City

In recent years, the work of quality supervision and production safety of aquatic products in Nanjing City has been carried out rapidly, and the system of "origin exit, market access, sign traceability, real-time monitoring" has been basically formed, initially realizing the whole-process monitoring on the quality safety of aquatic products from "pond to table". We take the current situation of quality supervision and production safety of aquatic products in Nanjing City as the study object, take the basic work of supervision on aquatic products in Nanjing City and advancing both in scope and in depth as breakthrough point, to sum up the results achieved in the work of quality supervision and production safety of aquatic products in Nanjing City in recent years; make initial exploration and research, in order to consolidate the existing achievements, and further enhance the level of supervision on quality safety of aquatic products in Nanjing City.

Adopting inclined channel to decline the salinity mixing for rotary energy recovery device:Simulation and optimization

After years of research,the energy efficiency of energy recovery device has been raised to a high level,but the salinity mixing has not been effectively improved.Mixing will lead to a rise of highpressure seawater salinity,which will increase the operating cost.In this paper,the computational fluid dynamics(CFD)simulation of the rotary energy recovery device(RERD)is carried out.It is found that the unstable flow caused by the nonparallel between the channel and the flow direction of fluid is an important reason for mixing.After the inclined channel structure is adopted,the nonparallel problem is improved.The formation of unstable flow is effectively controlled.Under the commercial product operating conditions,the volumetric mixing of the optimized device is reduced from 3.34%to 1.29%,showing the effectiveness of the structure.

腹膜后肿瘤切除术后胃排空障碍的危险因素分析

目的:探讨腹膜后肿瘤切除术后出现功能性胃排空障碍(functional delayed gastric emptying,FDGE)的危险因素。方法:回顾性分析2017年9月至2022年12月于北京大学国际医院行腹膜后肿瘤切除术后出现FDGE的患者67例(观察组),选择同期836例未出现FDGE的腹膜后肿瘤切除术患者作为对照组,调查和对比两组患者的病史及临床诊治资料,观察腹膜后肿瘤切除术后影响FDGE发生的危险因素。结果:全部患者总体术后FDGE发生率为(7.42%)。观察组存在不良精神因素(37.31%)、术前消化道梗阻(41.79%)、术后腹腔并发症(79.10%)、糖尿病(29.86%)的比例高于对照组,同时在年龄(61.85±6.11)岁、出血量(1011.94±507.30)mL方面高于对照组,差异具有统计学意义(P<0.05)。观察组术前白蛋白水平(38.22±3.75)g/L、术后白蛋白水平(30.22±3.36)g/L低于对照组,差异具有统计学意义(P<0.05)。危险因素分析显示,高龄、糖尿病、术前消化道梗阻、术中出血量、围手术期白蛋白水平、术后腹腔并发症、不良精神因素是术后发生FDGE的影响因素。结论:导致腹膜后肿瘤切除术后胃排空障碍的影响因素种类众多,应予以准确评估,制定合理、详细的预防和诊疗计划。

Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design

Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding.

Progress in Gel Polymer Electrolytes for Sodium-Ion Batteries

Sodium-ion battery is a potential application system for large-scale energy storage due to the advantage of higher nature abundance and lower production cost of sodium-based materials.However,there exist inevitably the safety problems such as flammability due to the use of the same type of organic liquid electrolyte with lithium-ion battery.Gel polymer electrolytes are being considered as an effective solution to replace conventional organic liquid electrolytes for building safer sodium-ion batteries.In this review paper,the authors present a comprehensive overview of the research progress in electrochemical and physical properties of the gel polymer electrolyte-based sodium batteries.The gel polymer electrolytes based on different polymer hosts namely poly(ethylene oxide),poly(acrylonitrile),poly(methyl methacrylate),poly(vinylidene fluoride),poly(vinylidene fluoride-hexafluoro propylene),and other new polymer networks are summarized.The ionic conductivity,ion transference number,electrochemical window,thermal stability,mechanical property,and interfacial issue with electrodes of gel polymer electrolytes,and the corresponding influence factors are described in detail.Furthermore,the ion transport pathway and ion conduction mechanism are analyzed and discussed.In addition,the advanced gel polymer electrolyte systems including flame-retardant polymer electrolytes,composite gel polymer electrolytes,copolymerization,single-ion conducting polymer electrolytes,etc.with more superior and functional performance are classified and summarized.Finally,the application prospects,development opportunities,remaining challenges,and possible solutions are discussed.

T cells expressing a LMP1-specific chimeric antigen receptor mediate antitumor effects against LMP1-positive nasopharyngeal carcinoma cells in vitro and in vivo

T cells modified with chimeric antigen receptor are an attractive strategy to treat Epstein-Barr virus（EBV） associated malignancies.The EBV latent membrane protein 1（LMP1） is a 66-KD integral membrane protein encoded by EBV that consists of transmembrane-spanning loops.Previously,we have identified a functional signal chain variable fragment（scFv） that specifically recognizes LMP1 through phage library screening.Here,we constructed a LMP1 specific chimeric antigen receptor containing anti-LMP1 scFv,the CD28 signalling domain,and the CD3ζchain（HELA/CAR）.We tested its functional ability to target LMP1 positive nasopharyngeal carcinoma cells.HELA/CAR cells were efficiently generated using lentivirus vector encoding the LMP1-specific chimeric antigen receptor to infect activated human CD3＋ T cells.The HELA/CAR T cells displayed LMP1 specific cytolytic action and produced IFN-γ and IL-2 in response to nasopharyngeal carcinoma cells overexpressing LMP1.To demonstrate in vivo anti-tumor activity,we tested the HELA/CAR T cells in a xenograft model using an LMP1 overexpressing tumor.Intratumoral injection of anti-LMP1 HELA/CAR-T cells significantly reduced tumor growth in vivo.These results show that targeting LMP1 using HELA/CAR cells could represent an alternative therapeutic approach for patients with EBV-positive cancers.

Less and shrunken pollen 1(LSP1) encodes a member of the ABC transporter family required for pollen wall development in rice(Oryza sativa L.)

Pollen fertility is an agronomic trait that strongly influences rice yield. Recent studies have revealed that the development of the pollen wall is required for pollen fertility and is regulated by several genes. However, the mechanisms underlying pollen and pollen wall development in rice remain largely unknown. In the present study, a point mutation in a gene on chromosome 1 was identified that resulted in the production of less and shrunken pollen(LSP) and led to defects in pollen wall formation. This gene was named LSP1 and was found to encode a member of the adenosine triphosphate-binding cassette(ABC)transporter G subfamily, OsABCG3. Two other loss-of-function mutants of LSP1/OsABCG3,generated using CRISPR/Cas9 technology, showed the same male sterile phenotype. The LSP1/OsABCG3 gene showed a spatio-temporal expression pattern in the developing anthers, and is an ortholog of the Arabidopsis genes At ABCG1 and At ABCG16, which play an important role in pollen wall development. Mutation of LSP1/OsABCG3 affected the expression of several genes involved in pollen and pollen wall formation. These results suggest that LSP1/OsABCG3 is critical for normal pollen fertility and shed light on the molecular mechanisms underlying rice pollen wall development.

Meta-analysis of Ginkgo biloba extract for the treatment of Alzheimer's disease

OBJECTIVE：To evaluate the effect of Ginkgo biloba extract on Alzheimer＇s disease using meta-analysis.DATA SOURCES：The following sources were used for articles concerning Ginkgo biloba extract for the treatment of Alzheimer＇s disease：Western biomedical journal literature databases,Chinese Biomedical Literature Database,Chinese Journal Full-text Database,Chinese Science and Technology Journal Full-text database.DATA SELECTION：Randomized controlled trials addressing Ginkgo biloba extract for the treatment of Alzheimer＇s disease were selected.The pathway and method of information collection were identical between treatment and control groups.Mild and moderate Alzheimer＇s disease patients scoring ≤ 26 points on the mini-mental state examination were included.Subjects met the diagnostic criteria for dementia by the American Psychiatric Association＇s ＂Diagnostic and Statistical Manual of Mental Disorders＂ Fourth revised edition.The quality of included literature was assessed by two authors.Meta-analysis was performed by RevMan4.2 software which was provided by the Cochrane Collaboration.Heterogeneity,sensitivity analysis,and bias evaluation were conducted.MAIN OUTCOME MEASURES：Scores of mini-mental state examination,ADAS-cog,and Syndrom-Kurztest.RESULTS：The five included randomized controlled trials contained 819 patients.Meta-analysis showed that the Syndrom-Kurztest score was significantly decreased（weighted mean difference =-2.32; 95%CI =-3.12,-1.52; P 〈 0.01） compared with the control group.No significant difference was found in the mini-mental state examination and ADAS-cog score（P 〉 0.05）.However,there was a tendency to elevate mini-mental state examination score and to reduce the ADAS-cog score.CONCLUSION：Ginkgo biloba extract shows good therapeutic effects for mild and moderate Alzheimer＇s disease.However,high-quality,randomized,double-blind,and controlled trials are needed to further confirm its therapeutic effects.

Optimal dose of zinc supplementation for preventing aluminum-induced neurotoxicity in rats

Zinc supplementation can help maintain learning and memory function in rodents. In this study, we hypothesized that zinc supplementation could antagonize the neurotoxicity induced by aluminum in rats. Animals were fed a diet containing different doses of zinc （50, 100, 200 mg/kg） for 9 weeks, and orally administered aluminum chloride （300 mg/kg daily） from the third week for 7 consecutive weeks. Open-field behavioral test results showed that the number of rearings in the group given the 100 mg/kg zinc supplement was significantly increased compared with the group given the 50 mg/kg zinc supplement. Malondialdehyde content in the cerebrum was significantly decreased, while dopamine and 5-hydroxytryptamine levels were increased in the groups given the diet sup- plemented with 100 and 200 mg/kg zinc, compared with the group given the diet supplemented with 50 mg/kg zinc. The acetylcholinesterase activity in the cerebrum was significantly decreased in the group given the 100 mg/kg zinc supplement. Hematoxylin-eosin staining revealed evident patho- logical damage in the hippocampus of rats in the group given the diet supplemented with 50 mg/kg zinc, but the damage was attenuated in the groups given the diet supplemented with 100 and 200 mg/kg zinc. Our findings suggest that zinc is a potential neuroprotective agent against alumi-num-induced neurotoxicity in rats, and the optimal dosages are 100 and 200 mg/kg.

Design of the Rotation Angle Measurement System for the Atmospheric Dispersion Corrector (ADC)

In order to measure the rotation angle error of the worm and gear mechanisms in the atmospheric dispersion corrector( ADC),a novel measurement system based on the autocollimator is designed in this paper.Based on the position relations between the shaft and spot converged by rays of autocollimator,the rotation angle can be calculated quickly and conveniently using a brief algorithm. An optical wedge is introduced to the measurement system for suppressing the awful measuring error caused by the axial wobbly error. The measurement system can measure the shaft rotation angle at any rotation position,which is the novel usage of the two-dimensional autocollimator. Its optical layout is very simple. Only an optical wedge and two plane mirrors are needed besides the autocollimator. The measurement accuracy of the proposed method is less than±0.5 arcmin. In the measurement of two worms and gears mechanisms in ADC,the rotation angle error are±0.05° and ±0.07° respectively,which all satisfies the design demand( ±0.1°). The proposed measurement system can be also suits for some engineering fields.

Self-similarity of spanwise rotational motions’population trends in decelerating open-channel flow

Decelerating open-channel flow is a type of flow that gradually moves forward with decreasing velocity and increasing water depth.Although all flow parameters change along the streamwise direction,previous studies have revealed that these parameters’vertical distributions at different sections can be universally described with a single profile when being nondimensionalised by appropriate scales.This study focuses on the population trends of spanwise rotational motions at various sections along the main flow direction by particle imaging velocimetry(PIV)measurement.The wall-normal population distributions of density,radius,swirling strength,and convection velocity of the prograde and retrograde motions show similar trends in uniform open-channel flows.The dimensionless representation is invariant along the main flow direction.This study’s results indicate the self-similar characteristic of population trends of spanwise rotational motions prevails in decelerating open-channel flow.

PD-1/L1 inhibitors can improve but not replace chemotherapy for advanced urothelial carcinoma:A systematic review and network meta-analysis

Background:Programmed cell death-1/ligand 1 inhibitors are a new treatment strategy for advanced urothelial carcinoma.Therefore,a comparative evaluation of their efficacy and toxicity compared with chemotherapy is necessary.Methods:We comprehensively searched PubMed,Web of Science,Embase,and Cochrane Library databases and performed a meta-analysis of randomized controlled trials up to July 2021.We considered overall survival as the primary outcome,and progression-free survival,objective response rate,and treatment-related adverse events as secondary outcomes.Results:Overall,3584 patients from five studies were evaluated.Compared with first-line chemotherapy,programmed cell death-1/ligand 1 inhibitors were significantly associated with worse progression-free survival(p<0.001)and adverse objective response rates(p<0.001).However,the treatments were not significantly different in terms of overall survival(p=0.33).Compared with second-line chemotherapy,programmed cell death-1/ligand 1 inhibitors significantly improved overall survival(p<0.001),and there was no statistically significant difference in progression-free survival(p=0.89)or objective response rate(p=0.34).Compared with chemotherapy,programmed cell death-1/ligand 1 inhibitors were well tolerated(first-line chemotherapy:p<0.001;second-line chemotherapy:p<0.001).Conclusions:The efficacy of programmed cell death-1/ligand 1 inhibitors in patients with advanced urothelial carcinoma is not superior to that of first-line platinum-based chemotherapy but is better than second-line chemotherapy;however,programmed cell death-1/ligand 1 inhibitors are safer than first-and second-line chemotherapy and have a broader prospect for use in combination therapy.

Applying multi-scale simulations to materials research of nuclear fuels:A review

Computational simulation is an important technical means in research of nuclear fuel materials.Since nuclear fuel issues are inherently multi-scopic,it is imperative to study them with multi-scale simulation scheme.At present,the development of multi-scale simulation for nuclear fuel materials calls for a more systematic approach,in which lies the main purpose of this article.The most important thing in multi-scale simulation is to accurately formulate the goals to be achieved and the types of methods to be used.In this regard,we first summarize the basic principles and applicability of the simulation methods which are commonly used in nuclear fuel research and are based on different scales ranging from micro to macro,i.e.First-Principles(FP),Molecular Dynamics(MD),Kinetic Monte Carlo(KMC),Phase Field(PF),Rate Theory(RT),and Finite Element Method(FEM).And then we discuss the major material issues in this field,also ranging from micro-scale to macro-scale and covering both pellets and claddings,with emphasis on what simulation method would be most suitable for solving each of the issues.Finally,we give our prospective analysis and understanding about the feasible ways of multi-scale integration and relevant handicaps and challenges.

Approaches to Nanoparticle Labeling:A Review of Fluorescent,Radiological,and Metallic Techniques

Nanomaterials are widely used in commercial products,resulting in the release of nanoscale particles into the environment.This raises concerns about their potential exposure risks in complex biological matrices.Most attempts use engineered nanomaterials(ENMs)to mimic the biological behavior of nanoparticles in the environment,and labeling of ENMs by sensors is a commonly used approach for sensitive detection and tracking of ENMs in organisms.However,due to the distinct physicochemical properties of nanoparticles,different labeling approaches have been developed,each with varying applicability.In this Review,we summarize the three main types of labeling methods used for nanoparticles:fluorescent,radiological,and metallic labeling.We discuss their labeling mechanisms,efficiency,stability,target nanoparticles,and applicability in different organism models.Finally,we propose a labeling scheme for specific nanoparticles.Overall,this Review provides a comprehensive overview of the advances in nanoparticle labeling techniques and their potential applications in environmental and health studies.

Ordovician integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas

The Ordovician rocks on the Qinghai-Tibetan Plateau represent the oldest non-metamorphic strata,and are critical to understanding the history of regional geology and biotic evolution of the entire plateau.Strata of Floian,Darriwilian,Sandbian,Katian and Hirnantian are represented in the plateau with a hiatus of variable duration occurring underneath the basal Ordovician across the area.Five stratigraphical regions,including the Himalaya,Gangdise-Zayu,Qiangtang-Qamdo,Songpan-Garze,and Karakoram-Kunlun-Altun,are differentiated for the Ordovician strata,which are correlated with their equivalents in the Sibumasu,Indochina,Qaidam-Qilian,Tarim-Tianshan,and the Yangtze(western margin)stratigraphical regions.On the QinghaiTibetan Plateau,graptolites,conodonts,and cephalopods are the most common and useful fossils for the Ordovician biostratigraphy.The Ordovician biotas of the Qinghai-Tibetan Plateau bear some distinguishable palaeobiogeographical signatures,among which the cephalopods are characterized by the flourishing actinocerids of North China affinity in the Early-Middle Ordovician,and by the thriving lituitids and orthocerids of South China affinity in the Middle-Late Ordovician.Fossil occurrences and their palaeobiogeographical evolution provide critical evidence bearing on the reconstruction of the geological history of the Qinghai-Tibetan Plateau and surrounding terranes in northeastern peri-Gondwana.The stratigraphical successions of the Cambrian-Ordovician transition in the Himalaya and Lhasa and nearby Sibumasu terranes were significantly affected by the Kurgiakh Orogeny,which resulted in the extensive unconformity between the Ordovician and the underlying rocks in most areas of the Qinghai-Tibetan Plateau.In southern Xizang,a warm-water biota of Middle Ordovician age was recovered from oolitic limestones,suggesting a likely palaeogeographical location in low-latitudes near the equator.In the Himalaya and Sibumasu regions,the Upper Ordovician was typified by the occurrence of red carbonates with distinctive reticulate structures,which are correlative to their equivalents in the Yangtze region of South China,and might be deposited under similar geological conditions.The global end-Ordovician glaciation and sea-level drop likely caused the wide absence of late Katian strata in western Yunnan of China and the Shan State of Myanma,and may have also affected deposition in the Xainza and Nyalam areas of the QinghaiTibetan Plateau during this time interval.

一种通用静电组装策略用于印刷无机纳米颗粒及其在大面积钙钛矿太阳电池中的应用

无机纳米颗粒作为一种极具前景的材料,在钙钛矿太阳电池的传输层中得到了广泛应用.然而,无机纳米颗粒易于聚集的特性阻碍了其进一步的发展.在大面积制备过程中,无机纳米颗粒的聚集加剧了“咖啡环效应”,导致薄膜不均匀,使得底部界面性能较差,进而影响钙钛矿薄膜的结晶,最终导致整个器件性能下降.为解决这一问题,我们将一种带电聚合物一一聚乙烯亚胺引入至无机纳米颗粒溶液中.聚乙烯亚胺的带电性质对无机纳米颗粒的聚集起到了显著的抑制作用,此外,添加聚乙烯亚胺还增加了无机纳米颗粒溶液的粘度,有助于减少薄膜上的负面咖啡环效应.最终,我们在基于1-cm^(2)正式结构且使用SnO_(2)传输层的器件上实现了23.2%的光电转换效率.为进一步证明这一策略的普适性,我们还将此策略验证基于NiO_(x)传输层的1-cm^(2)反式器件,其转换效率超过20%.

Newborn screening with targeted sequencing:a multicenter investigation and a pilot clinical study in China

Different newborn screening(NBS) programs have been practiced in many countries since the 1960 s. It is of considerable interest whether next-generation sequencing is applicable in NBS. We have developed a panel of 465 causative genes for 596 early-onset, relatively high incidence, and potentially actionable severe inherited diseases in our Newborn Screening with Targeted Sequencing(NESTS) program to screen 11,484 babies in 8 Women and Children’s hospitals nationwide in China retrospectively. The positive rate from preliminary screening of NESTS was 7.85%(902/11,484). With 45.89%(414/902) follow-up of preliminary positive cases, the overall clinically confirmative diagnosis rate of monogenic disorders was 12.07%(50/414), estimating an average of 0.95%(7.85% × 12.07%) clinical diagnosis rate, suggesting that monogenic disorders account for a considerable proportion of birth defects. The disease/gene spectrum varied in different regions of China. NESTS was implemented in a hospital by screening 3923 newborns to evaluate its clinical application. The turn-around time of a primary report, including the sequencing period of < 7 days, was within 11 days by our automatic interpretation pipeline. Our results suggest that NESTS is feasible and cost-effective as a first-tier NBS program, which will change the status of current clinical practice of NBS in China.