耐氯聚酰胺复合纳滤膜的制备与性能

采用哌嗪(PIP)与均苯三甲酰氯(TMC)为单体以界面聚合制备聚酰胺复合纳滤膜,用戊二醛(GA)对聚酰胺复合纳滤膜进行交联处理,再浸入间氨基乙酰苯胺中进行接枝反应得到具有耐氯性的改性复合纳滤膜。通过红外光谱和固体表面电位Zeta电位分析仪表征,证明间氨基乙酰苯胺接枝到活性层表面,降低了复合膜的荷负电性,扫描电子显微镜显示改性膜呈现无孔和更加粗糙的状态,接触角测量表明改性提高了复合膜表面的亲水性。耐氯测试表明,室温下,在pH=4,浓度1g/L的NaClO溶液中浸泡10h后,改性膜的截留率由94.04%下降至91.81%,PIP-TMC复合纳滤膜的截留率由94.60%下降至78.85%。

Dynamic coupling of ferrihydrite transformation and associated arsenic desorption/redistribution mediated by sulfate-reducing bacteria

Sulfate-reducing bacteria play an important role in the geochemistry of iron(oxyhydr)oxide and arsenic(As)in natural environments;however,the associated reaction processes are yet to be fully understood.In this study,batch experiments coupled with geochemical,spectroscopic,microscopic,and thermodynamic analyses were conducted to investigate the dynamic coupling of ferrihydrite transformation and the associated As desorption/redistribution mediated by Desulfovibrio vulgaris(D.vulgaris).The results indicated that D.vulgaris could induce ferrihydrite transformation via S^(2-)-driven and direct reduction processes.In the absence of SO_(4)^(2-),D.vulgaris directly reduced ferrihydrite,and As desorption and re-sorption occurred simultaneously during the partial transformation of ferrihydrite to magnetite.The increase in SO_(4)^(2-)loading promoted the S^(2-)-driven reduction of ferrihydrite and accelerated the subsequent mineralogical transformation.In the low and medium SO_(4)^(2-)treatments,ferrihydrite was completely transformed to a mixture of magnetite and mackinawite,which increased the fraction of As in the residual phase and stabilized As.In the high SO_(4)^(2-)treatment,although the replacement of ferrihydrite by only mackinawite also increased the fraction of As in the residual phase,22.1%of the total As was released into the solution due to the poor adsorption affinity of As to mackinawite and the conversion of As^(5+)to As^(3+).The mechanisms of ferrihydrite reduction,mineralogy transformation,and As mobilization and redistribution mediated by sulfate-reducing bacteria are closely related to the surrounding SO_(4)^(2-)loadings.These results advance our understanding of the biogeochemical behavior of Fe,S,and As,and are helpful for the risk assessment and remediation of As contamination.

Clinical practice of emergency department-initiated extracorporeal cardiopulmonary resuscitation for cardiac arrest in adults

To the Editor:Cardiac arrest(CA)is one of the leading causes of adult death globally.According to the latest American Heart Association reports,[1]the incidences of Emergency Medical Service-assessed adult out-of-hospital cardiac arrest(OHCA)and in-hospital cardiac arrest(IHCA)were 140.7/100,000 and 10.2/1000,respectively.

Consensus on pediatric epilepsy Che up surgery for young children:an investigation by the China Association Against Epilepsy task force on epilepsy surgery

Researchers have widely acknowledged the therapeutic value of epilepsy surgery for drug-resistant epilepsy.None-theless,there is a substantial gap in the surgical treatment for appropriate candidates owing to several factors,particularly in the population of young children.To standardize the protocols of preoperative evaluation and sur-gery of young children for epilepsy surgery,the China Association Against Epilepsy has appointed an expert task force to standardize the protocols of preoperative evaluation and surgery in pediatric epilepsy patients.It adopted the modifed Delphi method and performed two rounds of surveys through an anonymous inquiry among 75 experts from four subgroups including pediatric neurologists,epileptologists,pediatric epilepsy surgeons,and functional neurosurgeons.The survey contents contained:(1)the participants,comprising children aged≤6 years;(2)adopted DRE definition proposed by the International League Against Epilepsy in 2010;and(3)investigated epilepsy surgery,principally referring to curative epilepsy surgeries.The neuromodulation therapies were excluded because of the differences in treatment mechanisms from the above-mentioned surgeries.According to the Delphi process,a con-sensus was achieved for most aspects by incorporating two rounds of surveys including preoperative assessment,sur-gical strategies and techniques,and perioperative and long-term postoperative management,despite controversial opinions on certain items.We hope the results of this consensus will improve the level of surgical treatment and man-agement of intractable epilepsy in young children.

Aggregation and stability of selenium nanoparticles: Complex roles of surface coating, electrolytes and natural organic matter

The application of selenium nanoparticles(SeNPs)as nanofertilizers may lead to the release of SeNPs into aquatic systems.However,the environmental behavior of SeNPs is rarely studied.In this study,using alginate-coated SeNPs(Alg-SeNPs)and polyvinyl alcohol-coated SeNPs(PVA-SeNPs)as models,we systematically investigated the aggregation and stability of SeNPs under various water conditions.PVA-SeNPs were highly stable in mono-and polyvalent electrolytes,probably due to the strong steric hindrance of the capping agent.Alg-SeNPs only suffered from a limited increase in size,even at 2500 mmol/L NaCl and 200 mmol/L MgCl_(2),while they underwent apparent aggregation in CaCl_(2) and LaCl_(3) solutions.The binding of Ca^(2+) and La^(3+) with the guluronic acid part in alginate induced the formation of cross-linking aggregates.Natural organic matter enhanced the stability of Alg-SeNPs in monovalent electrolytes,while accelerated the attachment of Alg-SeNPs in polyvalent electrolytes,due to the cation bridge effects.The long-term stability of SeNPs in natural water showed that the aggregation sizes of Alg-SeNPs and PVA-SeNPs increased to several hundreds of nanometers or above 10μm after 30 days,implying that SeNPs may be suspended in the water column or further settle down,depending on the surrounding water chemistry.The study may contribute to the deep insight into the fate and mobility of SeNPs in the aquatic environment.The varying fate of SeNPs in different natural waters also suggests that the risks of SeNPs to organisms living in diverse depths in the aquatic compartment should be concerned.

Manganese therapy for dyslipidemia and plaque reversal in murine models

Precise control of circulating lipid levels is vital in both health and disease.We recently uncovered that bulk lipids,transported by lipoproteins,enter the circulation initially via the coat protein complex II(COPII)in a condensation-dependent manner.Divalent manganese,acting as a signaling messenger,selectively controls COPII condensation to regulate lipid homeostasis in vivo.Here,we present evidence for a manganese-based therapy in murine models of hypolipidemia and hyperlipidemia.

Orlistat induces ferroptosis-like cell death of lung cancer cells

Aberrant de novo lipid synthesis is involved in the progression and treatment resistance of many types of cancers,including lung cancer;however,targeting the lipogenetic pathways for cancer therapy remains an unmet clinical need.In this study,we tested the anticancer activity of orlistat,an FDA-approved anti-obesity drug,in human and mouse cancer cells in vitro and in vivo,and we found that orlistat,as a single agent,inhibited the proliferation and viabilities of lung cancer cells and induced ferroptosis-like cell death in vitro.Mechanistically,we found that orlistat reduced the expression of GPX4,a central ferroptosis regulator,and induced lipid peroxidation.In addition,we systemically analyzed the genome-wide gene expression changes affected by orlistat treatment using RNA-seq and identified FAF2,a molecule regulating the lipid droplet homeostasis,as a novel target of orlistat.Moreover,in a mouse xenograft model,orlistat significantly inhibited tumor growth and reduced the tumor volumes compared with vehicle control(P<0.05).Our study showed a novel mechanism of the anticancer activity of orlistat and provided the rationale for repurposing this drug for the treatment of lung cancer and other types of cancer.

Value of stereoelectroencephalography(SEEG)-guided radiofrequency thermocoagulation in treating drug-resistant focal epilepsy

Stereoelectroencephalography(SEEG)has been widely used in the presurgical evaluation of patients with medically intractable epilepsy.In the past,SEEG was commonly used as a method for mapping and localizing the epileptogenic zone(EZ).Since 2004,several studies have been conducted to examine the effectiveness of SEEG-guided radiofrequency thermocoagulation(RF-TC)in treating refractory epilepsy.However,the seizure-free and responder rates varied greatly across studies.We aimed to analyze the outcome of 56 patients who were treated with SEEG-guided RF-TC to evaluate the effectiveness of this treatment.SEEG-guided RF-TC can be considered as a treatment for refractory epilepsy.However,due to its limited efficacy,SEEG-guided RF-TC might be regarded as a temporary treatment performed under SEEG rather than a promising treatment for refractory epilepsy.

Clinical features and surgical treatment of epilepsy after viral encephalitis

Encephalitis is an acute inflammatory process of the brain parenchyma,which is often caused by viral infection.It is an vital cause of acute symptomatic seizures and subsequent epilepsy.The incidence of unprovoked and recurrent seizures after previous infections of the central nervous system is high and accounts for1%~5%of the cases of epilepsy.Viral encephalitis(VE)is directly caused by viral infection.The occurrence of seizures after VE is associated with poor prognosis.In survivors of VE,among other neurological sequelae,the risk of developing epilepsy is increased10-fold.The risk of severe neurological sequelae after VE is particularly high in very young children.Studies on seizure occurrence,possible underlying mechanisms,clinical characteristics,and clinical treatment(especially surgical treatment)of VE have yielded only limited detailed data.We reviewed the most recent literature on the clinical features and surgical treatment of post-VE epilepsy.

Stereoelectroencephalography-guided radiofrequency thermocoagulation(SEEG-guided RF-TC) in patients with drug-resistant focal epilepsy

For some patients with drug-resistant focal epilepsy, we usually select conventional surgical resection, which has brought better outcomes. However, others are not eligible for a conventional open surgical resection of the epileptogenic zone because of the proximity of a functional area or the implication of a larger epileptogenic network. Initially, stereoelectroencephalography(SEEG) exploration was a method of electroencephalography recording that was used in the presurgical evaluation of epileptic patients with complex epilepsy. Later, intracerebral electrodes used for SEEG were applied to produce radio frequency thermocoagulation(RF-TC) in epileptic patients. SEEG-guided RF-TC has produced some promising results, especially in the last dacade. Now, it has become popular as a palliative treatment to reduce seizure frequency in patients with drug-resistant focal epilepsy. This article presents a review of SEEG-guided RF-TC.

Polarization de-multiplexing using a modified Kalman filter in CO-OFDM transmissions

We propose the modified Kalman filter(MKF) using the received signal for observation and constructing an inverse process of the conventional Kalman filter(CKF) for polarization de-multiplexing in coherent optical(CO) orthogonal frequency-division multiplexing(OFDM) transmissions. The MKF can avoid the convergence error problem in CKF without matrix inverse operation and has a faster converging speed and a much larger tolerance to the process and measurement noise covariance, about two orders of magnitude more than those of CKF. We experimentally demonstrate the 12 Gbaud OFDM signal transmission over 480 km standard singlemode fiber. The performance of MKF and CKF outperforms pilot-aided polarization de-multiplexing with better accuracy and nonlinearity tolerance.

Expert consensus on clinical applications of high-frequency oscillations in epilepsy

Studies in animal models of epilepsy and pre-surgical patients have unanimously found a strong correlation between high-frequency oscillations(HFOs,>80 Hz)and the epileptogenic zone,suggesting that HFOs can be a potential biomarker of epileptogenicity and epileptogenesis.This consensus includes the definition and standard detection techniques of HFOs,the localizing value of pathological HFOs for epileptic foci,and different ways to distinguish physiological from epileptic HFOs.The latest clinical applications of HFOs in epilepsy and the related findings are also discussed.HFOs will advance our understanding of the pathophysiology of epilepsy.

Two NIS1-like proteins from apple canker pathogen(Valsa mali)play distinct roles in plant recognition and pathogen virulence

Conserved effectors produced by phytopathogens play critical roles in plant-microbe interactions.NIS1-like proteins represent a newly identified family of effectors distributed in multiple fungal species.However,their biological functions in a majority of pathogenic fungi remain largely elusive and require further investigation.In this study,we characterized two NIS1-like proteins VmNIS1 and VmNIS2 from Valsa mali,the causal agent of apple Valsa canker.Both of these two proteins were predicted to be secreted.Using agroinfiltration,we found that VmNIS1 induced intense cell death,whereas VmNIS2 suppressed INF1 elicitin-triggered cell death in Nicotiana benthamiana.Treatment of N.benthamiana with VmNIS1 recombinant protein produced by Escherichia coli activated a series of immune responses and enhanced plant disease resistance against Phytophthora capsici.In contrast,VmNIS2 suppressed plant immune responses and promoted P.capsici infection when transiently expressed in N.benthamiana.Both VmNIS1 and VmNIS2 were shown to be highly induced at late stage of V.mali infection.By individually knocking out of these two genes in V.mali,however,only VmNIS2 was shown to be required for pathogen virulence as well as tolerance to oxidative stress.Notably,we further showed that C-terminal extension of VmNIS1 was essential for plant recognition and VmNIS2 may escape plant detection via sequence truncation.Our data collectively indicate that VmNIS1 and VmNIS2 play distinct roles in plant recognition and pathogen virulence,which provided new insights into the function of NIS1-like proteins in plant-microbe interactions.

Preoperative evaluation and surgical strategy for epileptic spasms in children

Epileptic spasm(ES)is one of the most common types of seizures in children.It is primarily characterized by brief axial contractions lasting less than 2 s and recurring in short clusters.It usually occurs in children of 3 to 12 months of age,although it can also occur after the age of 1 year.In general,children with ES develop other symptoms of epilepsy,such as tonic,tonic-clonic,or focal seizures,after 3 to 5 years of age.ES in children is often damaging and usually results in developmental regression.First-line treatments for spasm seizures include adrenocorticotropic hormone(ACTH)and vigabatrin.However,many patients fail to respond to these medications,and continued to have spasms associated with progressive neurodevelopmental degeneration.Therefore,it is important to consider whether children with drug resistance meet surgical indications to consider surgical treatment in such conditions.In this study,we reviewed and summarized the importance of preoperative evaluation in order to provide surgical options for treatment of children with ES.

Effects of aging on working memory performance and prefrontal cortex activity: A time-resolved spectroscopy study

Objective:This study aimed to employ time‐resolved spectroscopy(TRS)to explore age‐related differences in prefrontal cortex(PFC)activity while subjects performed a working memory task.Methods:We employed TRS to measure PFC activity in ten healthy younger and ten healthy older subjects while they performed a working memory(WM)task.All subjects performed the Sternberg test(ST)in which the memory‐set size varied between one and six digits.Using TRS,we recorded changes in cerebral blood oxygenation as a measure of changes in PFC activity during the task.In order to identify left/right asymmetry of PFC activity during the working memory task,we calculated the laterality score,i.e.,Δoxy‐Hb(rightΔoxy‐Hb—leftΔoxy‐Hb);positive values indicate greater activity in the right PFC,while negative values indicate greater activity in the left PFC.Results:During the ST,statistical analyses showed no significant differences between the younger and older groups in accuracy for low memory‐load and high memory‐load.In high memory‐load tasks,however,older subjects were slower than younger subjects(P<0.05).We found that the younger group showed right lateral responses with a stronger right than left activation in the frontal pole,whereas the older group showed bilateral responses(P<0.05).Conclusions:The present results are consistent with the hemispheric asymmetry reduction in older adults(HAROLD)model;working memory tasks cause asymmetrical PFC activation in younger adults,while older adults tend to show reduced hemispheric lateralization.

基于连接性构筑图谱的岛叶-岛盖癫痫发作症状学亚组

岛叶-岛盖癫痫发作的特点是多样性的症状学表现,这与岛叶参与多种功能和广泛的连接有关。文章旨在确定其症状学亚组,并将它们与基于连接性构筑进行分区的岛叶-岛盖亚区相联系。回顾性收集来自3个癫痫中心37例立体定向脑电图(SEEG)检查确定的岛叶-岛盖癫痫发作的一组大样本量患者资料。基于解剖和功能连接的新的脑网络组图谱(Brainnetome atlas,BNA)被用于分割岛叶-岛盖皮质。仔细阅览症状学和SEEG的变化并进行量化,用主成分分析和聚类分析将症状学特征与岛叶-岛盖亚区相关联。基于BNA沿前腹侧-后背侧轴确定了4个主要的症状学亚组:第1组特征是上腹感觉和或协调性姿势性运动行为,伴有或不伴有恐惧或愤怒,涉及前腹侧岛叶和颞叶内侧结构;第2组特征是听觉及对称性近端肌或轴肌强直,涉及后腹侧的颞盖;第3组特征是口面部和喉部症状,涉及岛叶-岛盖的中间区;第4组特征是躯体感觉,继之出现非协调性姿势性运动行为和双侧非对称性强直,涉及后背侧岛叶-岛盖并传播至额叶内侧面。前腹部的癫痫发作主要表现为边缘系统症状学,而更靠后背侧的症状学主要涉及感觉运动系统。主观症状具有特殊的组别区分意义。可根据临床症状学对岛叶-岛盖癫痫发作进行分类,并将其与连接性构筑亚区相关联,它们沿前腹侧-后背侧轴排列,这与细胞构筑的梯度而非岛叶的沟回解剖相一致。这为岛叶-岛盖癫痫的鉴别诊断和术前定位提供了新思路,同时也强调了在确定复杂症状学模式的神经相关性时考虑连接性构筑的重要性。

Tet methylcytosine dioxygenase 2(TET2)deficiency elicits EGFR-TKI(tyrosine kinase inhibitors)resistance in non-small cell lung cancer

Despite epidermal growth factor receptor(EGFR)tyrosine kinase inhibitors(TKl)have shown remarkable efficacy in patients with EGFR-mutant non-small cell lung cancer(NSCLC),acquired resistance inevitably develops,limiting clinical efficacy.We found that TET2 was poly-ubiquitinated by E3 ligase CUL7^(FBXW11) and degraded in EGFR-TKI resistant NSCLC ells.Genetic perturbationof TET2 rendered parental cells more tolerant to TKI treatment.TET2 was stabilized by MEK1 phosphorylation at Ser 1107,while MEK1 inactivation promoted its proteasome degradation by enhancing the recruitment of CUL7^(FBXW11),Loss of TET2 resulted in the upregulation of TNF/NF-kB signaling that confers the EGFR-TKI resistance.Genetic or pharmacological inhibition of NF-kB attenuate the TKI resistance both in vitro and in vivo.Our findings exemplified how a cell growth controlling kinase MEK1 leveraged the epigenetic homeostasis by regulating TET2,and demonstrated an alternative path of non-mutational acquired EGFR-TKI resistance modulated by TET2 deficiency.Therefore,combined strategy exploiting EGFR-TKI and inhibitors of TET2/NF-κB axis holds therapeutic potential for treating NSCLC patients who suffered from this resistance.