Covalent organic frameworks(COFs)have been increasingly used in capillary electrochromatography due to their excellent characteristics.In this work,hydrazine-linked TFPB-DHzDS(TFPB:1,3,5-tris(4-formylphenyl)benzene;DH...Covalent organic frameworks(COFs)have been increasingly used in capillary electrochromatography due to their excellent characteristics.In this work,hydrazine-linked TFPB-DHzDS(TFPB:1,3,5-tris(4-formylphenyl)benzene;DHzDS:2,5-bis(3-(ethylthio)propoxy)terephthalohydrazide)was first synthesized by a simpler and easier method at room temperature and introduced into capillary electrochromatography as coating material.The TFPB-DHzDS coated capillaries were prepared by an in-situ growth process at room temperature.After optimizing the coating concentration and experimental conditions of capillary electrochromatography,baseline separation of two groups of polycyclic aromatic hydrocarbons was achieved based on the TFPB-DHzDS coated capillary.And the established method was used successfully to determine PAHs in natural water and soil samples.The spiked recoveries of polycyclic aromatic hydrocarbons in these samples ranged from 90.01%to 111.0%,indicating that the method is reliable and could detect polycyclic aromatic hydrocarbons in natural samples.Finally,molecular simulation was applied to study and visualize the interaction between the analytes and coating COF materials to investigate the molecular level separation mechanism further.展开更多
In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize op...In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography(OT-CEC)enantioseparation.The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters,all achieving good resolution and symmetrical peak shape.For three consecutive runs,the relative standard deviations(RSD)of migration time for intra-day,inter-day,and column-tocolumn repeatability were in the range of 0.3%-3.5%,0.2%-4.9%and 2.1%-7.7%,respectively.Moreover,the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency.Therefore,the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.展开更多
基金the National Natural Science Foundation of China(Grant Nos.21705064,21675068)for financial support.
文摘Covalent organic frameworks(COFs)have been increasingly used in capillary electrochromatography due to their excellent characteristics.In this work,hydrazine-linked TFPB-DHzDS(TFPB:1,3,5-tris(4-formylphenyl)benzene;DHzDS:2,5-bis(3-(ethylthio)propoxy)terephthalohydrazide)was first synthesized by a simpler and easier method at room temperature and introduced into capillary electrochromatography as coating material.The TFPB-DHzDS coated capillaries were prepared by an in-situ growth process at room temperature.After optimizing the coating concentration and experimental conditions of capillary electrochromatography,baseline separation of two groups of polycyclic aromatic hydrocarbons was achieved based on the TFPB-DHzDS coated capillary.And the established method was used successfully to determine PAHs in natural water and soil samples.The spiked recoveries of polycyclic aromatic hydrocarbons in these samples ranged from 90.01%to 111.0%,indicating that the method is reliable and could detect polycyclic aromatic hydrocarbons in natural samples.Finally,molecular simulation was applied to study and visualize the interaction between the analytes and coating COF materials to investigate the molecular level separation mechanism further.
基金the financial support from the National Natural Science Foundation of China(No.21874060)the Special Funding for Open and Shared Large-Scale Instruments and Equipments of Lanzhou University(No.LZU-GXJJ-2019C038)。
文摘In this wo rk,the phase-transitioned BSA(PTB)film using the mild and fast fabrication process adhered to the capillary inner wall uniformly,and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography(OT-CEC)enantioseparation.The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters,all achieving good resolution and symmetrical peak shape.For three consecutive runs,the relative standard deviations(RSD)of migration time for intra-day,inter-day,and column-tocolumn repeatability were in the range of 0.3%-3.5%,0.2%-4.9%and 2.1%-7.7%,respectively.Moreover,the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency.Therefore,the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.
