三种同分异构的双苯并吩噻嗪材料的合成、理论计算及光物理性质

吩噻嗪及其衍生物材料是一类重要的多环芳烃材料,在光电子领域有着广泛的应用。其中,基于苯并噻嗪材料的研究相对较少。在本文中,我们分别在吩噻嗪的1,2-、2,3-和3,4-位引入苯基,制备了三种同分异构的双苯并吩噻嗪化合物D-PTZa、D-PTZb和D-PTZc,研究了它们的构效关系,并与双吩噻嗪化合物(D-PTZ)进行了对比。研究发现,D-PTZb和D-PTZc的HOMO和LUMO分布与D-PTZ的类似;对于D-PTZa,其1,2-位引入的苯基与中间的苯环空间张力较大,造成空间结构极度扭曲,性质比较特殊。并苯的引入可以有效增加分子的共轭长度,使得最大吸收波长发生红移;在2,3-位引入苯基可以有效地稳定HOMO能级,使基于π→π*跃迁的能隙稍有增大,呈现蓝光发射,溶液的荧光量子产率为1.7%;而在3,4-位引入苯基使LUMO分布更加趋向于线型,从而使LUMO更加稳定,使基于π→π*跃迁的能隙降低,其最大发射峰位于520 nm处,呈现黄绿光发射,溶液荧光量子产率为13%。此外并入苯环之后,空间张力增大,化合物的分解温度降低。我们的分子设计和结构–性质关系的研究可以为设计新的吩噻嗪材料提供基础指导。

Network pharmacology and subsequent experimental validation reveal the synergistic myocardial protection mechanism of Salvia miltiorrhiza Bge.and Carthamus tinctorius L.

Objective:To reveal the molecular mechanism underlying the compatibility of Salvia miltiorrhiza Bge(S.miltiorrhiza,Dan Shen)and C.tinctorius L.(C.tinctorius,Hong Hua)as an herb pair through network pharmacology and subsequent experimental validation.Methods:Network pharmacology was applied to construct an active ingredient-efficacy target-disease protein network to reveal the unique regulation pattern of s.miltiorrhiza and C.tinctorius as herb pair.Molecular docking was used to verify the binding of the components of these herbs and their potential targets.An H9c2 glucose hypoxia model was used to evaluate the efficacy of the components and their synergistic effects,which were evaluated using the combination index.Western blot was performed to detect the protein expression of these targets.Results:Network pharmacology analysis revealed 5 pathways and 8 core targets of s.miltiorrhiza and C.tinctorius in myocardial protection.Five of the core targets were enriched in the hypoxia-inducible factor-1(HIF-1)signaling pathway.S.miltiorrhiza-C.tinctorius achieved vascular tone mainly by regulating the target genes of the HIF-1 pathway.As an upstream gene of the HIF-1 pathway,STAT3 can be activated by the active ingredients cryptotanshinone(Ctan),salvianolic acid B(Sal.B),and myricetin(Myric).Cell experiments revealed that Myric,Sal.B,and Ctan also exhibited synergistic myocardial protective activity.Molecular docking verified the strong binding of Myric,Sal.B,and Ctan to STAT3.Western blot further showed that the active ingredients synergistically upregulated the protein expressionof STAT3.Conclusion:The pharmacodynamic transmission analysis revealed that the active ingredients of S.miltiorrhiza and C.tinctorius can synergistically resist ischemia through various targets and pathways.This study provides a methodological reference for interpreting traditional Chinese medicine compatibility.

近十年国际跨文化传播研究进展的文献计量分析

本文采用文献计量方法,对近十年SSCI期刊收录的跨文化传播研究论文进行统计分析。分析结果表明:1.国外核心期刊跨文化研究发表数量总体呈上升趋势;2.相关论文覆盖学科广泛、跨学科特性突出;3.跨文化教育与留学为最热研究议题,跨文化适应和移民、跨文化传播动态和文化认同等主题越来越受关注;4.应用最广的跨文化理论包括跨文化能力和敏感度理论、跨文化与外语教学理论、文化维度理论等。最后,研究者依据调研结果总结相应启示。

幼儿园课程价值的达成路径:内化于心与外化于行

幼儿园课程的根本价值在于促进幼儿身心和谐发展,把幼儿培养成为一个内外兼修且具有良好社会适应能力的个体。幼儿园课程价值的达成要强调幼儿对课程目标的内化以及外在行为习惯的培养。幼儿只有在获得丰富课程经验的基础上,才能对课程目标产生深刻的体验和认同。为了更好地实现课程的价值目标,教师在课程实践中应充分发挥引导作用,通过组织良好的教育活动和教师行为示范促进幼儿的整全发展。

用于食品中玉米赤霉烯酮高灵敏、快速检测的倏逝波免疫传感技术

玉米赤霉烯酮(zearalenone,ZEN)是一种由镰刀菌属产生的次级代谢产物,利用课题组自主研发的倏逝波光纤免疫传感器,以玉米赤霉烯酮包被抗原修饰的光纤探头作为生物识别元件,基于间接竞争免疫分析原理,建立了ZEN的高灵敏、快速定量检测方法。在最佳检测条件下,ZEN的检测限为0.35μg·L^(−1),线性区间为0.75—7.48μg·L^(−1),单个检测周期只需8 min。包被抗原修饰的光纤探头可重复使用100次以上而没有明显的性能损失,可以保证检测结果的准确性并降低成本。对小麦、玉米、猪饲料进行了不同浓度的加标回收测试,结果显示加标回收率在80.86%—113.92%范围内,相对标准偏差小于7.90%,表明该传感器具有很好的稳定性和精确度,可以用于食品中ZEN的快速检测,为食品安全评价提供有力分析工具。

Organophosphine ligand derived sandwich-structural electrocatalyst for oxygen evolution reaction

The synchronous construction of metal phosphate and phosphorus-doped carbon structures is of great significance to innovate the design,synthesis,and application of catalysts,as these phosphoruscontaining composite materials have shown a remarkable contribution to electrocatalysts.However,their preparation procedure generally involves using large amounts of excess phosphorus sources for phosphorization,which inevitably release poisonous PH_(3) or dangerous phosphorus vapor.Here,a strategy for in-situ formation of FePO_(4) embedded in P-doped carbon 2D nano film(FePO_(4)/PdC)is developed using a highly integrated precursor,which is a small molecular organophosphine ligand,1,1’bis(diphenylphosphine)ferrocene(DPPF).The multi-source precursor DPPF that contains Fe,P,and C is molecular-vapor-deposited on the nickel foam(NF)supported ZIF-67 nanosheets to obtain the composite catalyst,namely DPPF-500/ZIF-67/NF.FePO_(4)/PdC encapsulated the ZIF-67 derived Co/N-doped carbon matrix(Co NC)to form a sandwich structure FePO_(4)/PdC@CoNC.The constructed catalyst shows good performance for OER,requiring an overpotential of only 297 m V to deliver 600 m A/cm^(2) with a Tafel slope of 42.7 m V dec^(-1).DFT calculations demonstrate that the synergistic effects between the metal active center and P-doped carbon film reduce the energy barriers and improve electron transport.This method of constructing P-containing catalysts overcomes the demand for additional P sources to realize eco-friendly fabrication and yields a unique structure with good catalytic activity.

Modified enzymatic collagen digestion-mediated isolation of osteocytes

This study established a method for isolating large numbers of high-purity osteocytes from high-density bone.Bone fragments derived from mice tibia and femurs were alternately digested with type I collagenase and EDTA nine times,and the digested cells and bone chips(BC)were cultured,digested,and passaged when cells were fully grown.The types of cells obtained were identified by morphology,viable cell counts,alkaline phosphatase staining,and biochemical activity analyses,and specific osteocyte and osteoblast markers were evaluated by quantitative real-time polymerase chain reaction.Our results showed that among the cells obtained from the third digestion(fractions 7–9)of wild mice tibias and femurs and the remaining BCs,85%–90%of the cells were osteocytes.Moreover,their morphology was approximately one-tenth to one-fifth the size of osteoblasts,star-shaped or polygonal,with a dendritic structure,negative for alkaline phosphatase staining,and showed a high expression of dmp1 and sclerostin.Ninety percent of the cells in fractions 1–3 were osteoblasts,and were fusiform or polygonal shape.The activity of osteoblast-specific alkaline phosphatase and mRNA expression were high in this fraction,while the expression of osteocyte-specific dmp1 and sclerostin was not detected.In the second portion(fractions 4–6),a large number were osteoblasts,mixed with a small number of osteocytes,and had high alkaline phosphatase activity and osteocyte mRNA levels,a specific level of the osteocyte marker dmp1,and no sclerostin was detected.Osteocytes in daβcatot mice were also successfully isolated by this method,and we found that Wnt signaling increased the proliferation of these osteocytes.The proposed method can be used to culture osteocytes and osteoblasts of high purity and can be used for isolation and culture of these two kinds of cells from high-density bone,which provides an avenue for the study of osteocyte function in vitro.

Resolution of Symptoms after Parent Artery Occlusion Treatment for Giant Cavernous Carotid Artery Aneurysms

Background and Objective: Giant cavernous carotid artery aneurysms (CCAAs) often produce a variety of neurological deficits, primarily those related to ophthalmoplegia/paresis and headache. This study was designed to evaluate the resolution of symptoms after parent artery occlusion (PAO) treatment for giant CCAAs. Methods: We retrospectively reviewed a series of 17 consecutive giant CCAAs treated with PAO treatment. All patients were evaluated by balloon occlusion test (BOT) before treatment. Patients who could tolerate BOT were treated by PAO. The following outcomes were analyzed: angiographic assessment, evolution of symptoms and outcome at clinical follow-up using modified Rankin Scale (mRS). Results: A total number of 17 giant CCAAs were treated by PAO. The initial post-procedure and follow-up angiogram revealed complete occlusion in all patients, no new lesion was detected. Periprocedural infarcts occurred in 1 patient (5.9%). Procedure-related mortality and morbidity were 0% and 5.9%, respectively. At mean 31.8 months clinical follow-up, symptoms had disappeared in 7 (41.2%) of the patients, partially improved in 5 (29.4%), remained unchanged in 4 (23.5%) and worsened in 1 (5.9%) of cases. Sixteen (94.1%) patients presented a good clinical outcome (mRS 0 - 1). Conclusion: Most patients in our series improved or remained stable after PAO. The results of this study indicate that PAO can improve the outcome of those symptomatic giant CCAAs if BOT can be tolerated.

A multi-step induced strategy to fabricate core-shell Pt-Ni alloy as symmetric electrocatalysts for overall water splitting

Devising an electrocatalyst with brilliant efficiency and satisfactory durability for hydrogen production is of considerable demand,especially for large-scale application.Herein,we adopt a multi-step consequential induced strategy to construct a bifunctional electrocatalyst for the overall water splitting.Graphene oxide(GO)was used as a carbon matrix and in situ oxygen source,which was supported by the octahedral PtNi alloy to form the PtxNiy-GO precursor.When calcinating in Ar atmosphere,the oxygen in GO induced the surface segregation of Ni from the PtNi octahedron to form a core-shell structure of Ptx@Niy.Then,the surface-enriched Ni continuously induced the reformation of C in reduced graphene oxide(rGO)to enhance the degree of graphitization.This multi-step induction formed a nanocatalyst Pt_(x)@Ni_(y)-rGO which has very high catalytic efficiency and stability.By optimizing the feeding ratio of PtNi(Pt:Ni=1:2),the electrolytic overall water splitting at 10 mA·cm^(-2) can be driven by an electrolytic voltage of as low as 1.485 V,and hydrogen evolution reaction(HER)only needs an overpotential of 37 mV in 1.0 M KOH aqueous solution.Additionally,the catalyst exhibited consistent existence form in both HER and oxygen evolution reaction(OER),which was verified by switching the anode and cathode of the cell in the electrolysis of water.This work provides a new idea for the synthesis and evaluation of the bifunctional catalysts for water splitting.

具有可调节三阶非线性光学性能的偶氮苯金属配合物

具有刺激响应性的三阶非线性光学材料由于其可控的光物理性质而受到了广泛的关注.本文设计并合成了两种少见的金属配合物三阶非线性光开关,它们在性能转换、多功能性和响应速度等方面远优于同类光开关材料.三阶非线性光学性能测试结果表明,该金属配合物在光照前同时具有反饱和吸收和自散焦折射行为.而在光照后,吸收行为立即消失且自散焦折射行为迅速转变为自聚焦折射.我们利用核磁共振技术和紫外可见吸收光谱研究了分子结构和三阶非线性光学性能之间的关系并探究了金属离子对三阶非线性光学行为产生的影响.此外,我们还通过泵浦探测技术和密度泛函理论计算揭示了金属配合物的非线性光学性能产生的机制.总之,这两种金属配合物不仅可以作为理想的三阶非线性光开关材料,而且也能够扩展具有可调节三阶非线性光学性能材料的应用范围.

Preparation of high performance YGdBCO films by low fluorine TFA-MOD process

YBCO films doped with different contents of gadolinium(Gd) were prepared by the low-fluorine(low-F)trifluoroacetate metal-organic deposition(MOD) method.The effects of flow rate and holding time of the firing(crystallization) stage on the superconducting properties of YxGd1-xBa2 Cu3 O7-δ(YGdBCO) films were investigated.The phase formation and texture were characterized by the X-ray diffraction(XRD),which indicate that severe degradation of the microstructure will be induced with the inappropriate flow rate.The surface morphology and element distribution were investigated by the scanning electron microscopy(SEM) and energy dispersive spectroscopy(EDS).The results show that increasing the holding time of the firing stage is effective for the further decomposition of residual impurity phase on the surface.The mechanisms of the phase and surface evolution are also discussed.Finally,a high critical current density(Jc) value of 5.4 MA/cm2 was achieved in the Y0.9Gd0.1BCO film fabricated by the cooperative control of the flow rate and holding time of the firing stage,which are contributed to the formation of excellent texture,homogeneous microstructure and dense surface of the YGdBCO films.

NIR-Ⅱprobe modified by poly(L-lysine)with efficient ovalbumin delivery for dendritic cell tracking

Dendritic cell(DC)vaccine is an effective strategy for cancer immunotherapy by carrying antigen into DCs and migrating these DCs to drain lymph nodes after inoculation.In this article,second near-infrared window(NIR-II)fluorescent nanoparticles have been used to uptake antigen and activate DCs.Ovalbumin(OVA),an antigen for immunization,can be loaded on the surface of these NIR-II fluorescent nanoparticles via electrostatic interaction by virtue of their functionalized poly(L-lysine)(PLL),which exhibits biocompatibility and strong selective interaction with OVA.In addition,these antigen-loaded complexes can efficiently be engulfed by immature DCs to induce DC maturation and cytokine secretion.After subcutaneous injection,highly sensitive NIR-II fluorescence signal from nanoparticles indicates that nanoparticle-labeled DCs can successfully migrate into lymph nodes in vivo,showing great promise in immunotherapy against cancer.

Analyzing the interdisciplinarity of Big Data research

Big Data is a hot topic at present,and many disciplines are engaged in it.However,the relationship and status of these disciplines in Big Data research are still not well understood.Based on bibliometric data from the Web of Science,this study analyzes the interdisciplinarity of Big Data research.It focuses on its spatial and temporal distribution characteristics by combining traditional bibliometrics,interdisciplinarity indicators,and so cial network analysis.We found that:1)In recent years,the research topics of Big Data include the Internet of Things,Syndromic Surveillance,Knowledge M anagement,Industry 4.0,etc.M ost Big Data research comes from China,the United States,and India.2)Big Data research involved 141 disciplines,but the disciplinary distribution is unbalanced,among which the diversity of disciplines is constantly improving;The balance of disciplines is on the rise,and the situation that is dominated by a few disciplines has been improved;The disparity of disciplines increases and researchers tend to cite the disciplines that are quite different from their own disciplines.3)Computer Science and Engineering are the two most important disciplines in the Big Data field.According to the collaboration degree,the interdisciplinary collaboration network can be divided into five communities:Community 1,represented by Computer Science and Engineering;Community 2,represented by Business&Economics;Community 3,represented by Science&Technology;Community 4,represented by M aterials Science;Community 5,represented by Health Care Sciences&Services.The collaboration between a few key disciplines has changed,and the whole network is still expanding in Big Data research.