[Objectives] To analyze the quality of Coptis chinensis grown under woods of Magnolia officinalis Rehd. et Wils.,Platycladus orientalis( Linn.) Franco.,and Cunninghamia lanceolata( Lamb.). [Methods] The content of fiv...[Objectives] To analyze the quality of Coptis chinensis grown under woods of Magnolia officinalis Rehd. et Wils.,Platycladus orientalis( Linn.) Franco.,and Cunninghamia lanceolata( Lamb.). [Methods] The content of five kinds of alkaloids in C. chinensis grown under three different woods was determined by high-performance liquid chromatography( HPLC). Detection conditions: 0. 03 mol/L methanol and potassium dihydrogen phosphate solution( volume ratio of 65∶ 35) as the mobile phase,flow rate of 1. 0 mL/min,detection wavelength of 350 nm,and column temperature of 35℃. [Results] In these three woods,the quality of C. chinensis grown under P. orientalis woods was the highest. [Conclusions] This study is expected to provide certain references for cultivation and planting of C. chinensis under woods.展开更多
Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogeni...Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogenic bacteria can directly or indirectly promote the occurrence of kidney stones.However,currently existing natural probiotics have limitations.Synthetic biology is an emerging discipline in which cells or living organisms are genetically designed and modified to have biological functions that meet human needs,or even create new biological systems,and has now become a research hotspot in various fields.Using synthetic biology approaches of microbial engineering and biological redesign to enable probiotic bacteria to acquire new phenotypes or heterologous protein expression capabilities is an important part of synthetic biology research.Synthetic biology modification of microorganisms in the gut and urinary tract can effectively inhibit the development of kidney stones by a range of means,including direct degradation of metabolites that promote stone production or indirect regulation of flora homeostasis.This article reviews the research status of engineered microorganisms in the prevention and treatment of kidney stones,to provide a new and effective idea for the prevention and treatment of kidney stones.展开更多
文摘[Objectives] To analyze the quality of Coptis chinensis grown under woods of Magnolia officinalis Rehd. et Wils.,Platycladus orientalis( Linn.) Franco.,and Cunninghamia lanceolata( Lamb.). [Methods] The content of five kinds of alkaloids in C. chinensis grown under three different woods was determined by high-performance liquid chromatography( HPLC). Detection conditions: 0. 03 mol/L methanol and potassium dihydrogen phosphate solution( volume ratio of 65∶ 35) as the mobile phase,flow rate of 1. 0 mL/min,detection wavelength of 350 nm,and column temperature of 35℃. [Results] In these three woods,the quality of C. chinensis grown under P. orientalis woods was the highest. [Conclusions] This study is expected to provide certain references for cultivation and planting of C. chinensis under woods.
文摘Numerous studies have shown that intestinal and urinary tract flora are closely related to the formation of kidney stones.The removal of probiotics represented by lactic acid bacteria and the colonization of pathogenic bacteria can directly or indirectly promote the occurrence of kidney stones.However,currently existing natural probiotics have limitations.Synthetic biology is an emerging discipline in which cells or living organisms are genetically designed and modified to have biological functions that meet human needs,or even create new biological systems,and has now become a research hotspot in various fields.Using synthetic biology approaches of microbial engineering and biological redesign to enable probiotic bacteria to acquire new phenotypes or heterologous protein expression capabilities is an important part of synthetic biology research.Synthetic biology modification of microorganisms in the gut and urinary tract can effectively inhibit the development of kidney stones by a range of means,including direct degradation of metabolites that promote stone production or indirect regulation of flora homeostasis.This article reviews the research status of engineered microorganisms in the prevention and treatment of kidney stones,to provide a new and effective idea for the prevention and treatment of kidney stones.
