The influence factors and paths of methane formation during methanol to hydrocarbons (MTH) reaction were studied experimentally and thermodynamically. The fixed-bed reaction results show that the formation of methan...The influence factors and paths of methane formation during methanol to hydrocarbons (MTH) reaction were studied experimentally and thermodynamically. The fixed-bed reaction results show that the formation of methane was favored by not only high temperature, but also high feed velocity, low pressure, as well as weak acid sites dominated on deactivated catalyst. The thermodynamic analysis results indicate that methane would be formed via the decomposition reactions of methanol and DME, and the hydrogenolysis reactions of methanol and DME. The decomposition reactions are thermal chemistry processes and easily occurred at high temperature. However, they are influenced by catalyst and reaction conditions through DME intermediate. By contrast, the hydrogenolysis reactions belong to catalytic processes. Parallel experiments suggest that, in real MTH reactions, the hydrogenolysis reactions should be mainly enabled by surface active H atom which might come from hydrogen transfer reactions such as aromatization. But H2 will be involved if the catalyst has active components like NiO.展开更多
The presence of protein aggregates in numerous human diseases underscores the significance of detecting these aggregates to comprehend disease mechanisms and develop novel therapeutic approaches for combating these di...The presence of protein aggregates in numerous human diseases underscores the significance of detecting these aggregates to comprehend disease mechanisms and develop novel therapeutic approaches for combating these disorders.Despite the development of various biosensors and fluorescent probes that selectively target amyloid fibers or amorphous aggregates,there is still a lack of tools capable of simultaneously detecting both types of aggregates.Herein,we demonstrate the quantitative discernment of amorphous aggregates by QM-FN-SO3,an aggregationinduced emission(AIE)probe initially designed for detecting amyloid fibers.This probe easily penetrates the membranes of the widely-used prokaryotic model organism Escherichia coli,enabling the visualization of both amorphous aggregates and amyloid fibers through near-infrared fluorescence.Notably,the probe exhibits sensitivity in distinguishing the varying aggregation propensities of proteins,regardless of whether they form amorphous aggregates or amyloid fibers in vivo.These properties contribute to the successful application of the QM-FN-SO3 probe in the subsequent investigation of the antiaggregation activities of two outer membrane protein(OMP)chaperones,both in vitro and in their physiological environment.Overall,our work introduces a near-infrared fluorescent chemical probe that can quantitatively detect amyloid fibers and amorphous aggregates with high sensitivity in vitro and in vivo.Furthermore,it demonstrates the applicability of the probe in chaperone biology and its potential as a high-throughput screening tool for protein aggregation inhibitors and folding factors.展开更多
Two phase transition compounds,diethylammonium 1,5-naphthalenedisulfonate(1)and cyclohexylammonium 1,5-naphthalenedisulfonate(2),were screened from a series of organic salts based on 1,5-naphthalenedisulfonate.The pha...Two phase transition compounds,diethylammonium 1,5-naphthalenedisulfonate(1)and cyclohexylammonium 1,5-naphthalenedisulfonate(2),were screened from a series of organic salts based on 1,5-naphthalenedisulfonate.The phase transition behaviors were studied by differential scanning calorimetry,single-crystal X-ray analysis and dielectric measurements.Compounds 1 and 2 undergo phase transitions at about 202 and 148 K,respectively,accompanied by distinct dielectric changes.The origin of the phase transitions was ascribed to motional changes of the cations in the crystal lattices.展开更多
Up to know,product information for COVID-19 mRNA vaccines do not include their use in patients receiving immunosuppressive medication,since they were not included in trials(1).For the BNT162b2 mRNA vaccine,one case of...Up to know,product information for COVID-19 mRNA vaccines do not include their use in patients receiving immunosuppressive medication,since they were not included in trials(1).For the BNT162b2 mRNA vaccine,one case of otherwise totally asymptomatic elevations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)andγ-glutamyl transpeptidase(GGT)serum concentrations in a liver transplant recipient has been reported after the first injection(2).In the present retrospective study,we present safety outcomes of COVID-19 vaccinations in 56 liver transplant patients from two Chinese centers vaccinated with the domestic PiCoVacc,BBIBP-CorV and ZF2001 vaccines.Thirty vaccinated physicians from the Hepatopancreatobiliary Center of Beijing Tsinghua Changgung Hospital served as control,from which 1 participant received an Ad5-nCoV vaccine.The study deadline was 2021-10-31.The observation period for adverse reactions,such as muscle pain and fatigue was 1 week,while for laboratory examinations such as liver function the follow-up period was 1 month.展开更多
文摘The influence factors and paths of methane formation during methanol to hydrocarbons (MTH) reaction were studied experimentally and thermodynamically. The fixed-bed reaction results show that the formation of methane was favored by not only high temperature, but also high feed velocity, low pressure, as well as weak acid sites dominated on deactivated catalyst. The thermodynamic analysis results indicate that methane would be formed via the decomposition reactions of methanol and DME, and the hydrogenolysis reactions of methanol and DME. The decomposition reactions are thermal chemistry processes and easily occurred at high temperature. However, they are influenced by catalyst and reaction conditions through DME intermediate. By contrast, the hydrogenolysis reactions belong to catalytic processes. Parallel experiments suggest that, in real MTH reactions, the hydrogenolysis reactions should be mainly enabled by surface active H atom which might come from hydrogen transfer reactions such as aromatization. But H2 will be involved if the catalyst has active components like NiO.
基金Natural Science Foundation of Shanghai,Grant/Award Number:23ZR1415300National Natural Science Foundation of China,Grant/Award Numbers:32222049,31661143021,32171269,32201043+1 种基金National Key Research and Development Program of China,Grant/Award Number:2022YFF1102900Shanghai Frontier Science Center of Optogenetic Techniques for Cell Metabolism Shanghai Municipal Education Commission,Grant/Award Number:2021Sci&Tech0328。
文摘The presence of protein aggregates in numerous human diseases underscores the significance of detecting these aggregates to comprehend disease mechanisms and develop novel therapeutic approaches for combating these disorders.Despite the development of various biosensors and fluorescent probes that selectively target amyloid fibers or amorphous aggregates,there is still a lack of tools capable of simultaneously detecting both types of aggregates.Herein,we demonstrate the quantitative discernment of amorphous aggregates by QM-FN-SO3,an aggregationinduced emission(AIE)probe initially designed for detecting amyloid fibers.This probe easily penetrates the membranes of the widely-used prokaryotic model organism Escherichia coli,enabling the visualization of both amorphous aggregates and amyloid fibers through near-infrared fluorescence.Notably,the probe exhibits sensitivity in distinguishing the varying aggregation propensities of proteins,regardless of whether they form amorphous aggregates or amyloid fibers in vivo.These properties contribute to the successful application of the QM-FN-SO3 probe in the subsequent investigation of the antiaggregation activities of two outer membrane protein(OMP)chaperones,both in vitro and in their physiological environment.Overall,our work introduces a near-infrared fluorescent chemical probe that can quantitatively detect amyloid fibers and amorphous aggregates with high sensitivity in vitro and in vivo.Furthermore,it demonstrates the applicability of the probe in chaperone biology and its potential as a high-throughput screening tool for protein aggregation inhibitors and folding factors.
基金the National Natural Science Foundation of China (21225102)
文摘Two phase transition compounds,diethylammonium 1,5-naphthalenedisulfonate(1)and cyclohexylammonium 1,5-naphthalenedisulfonate(2),were screened from a series of organic salts based on 1,5-naphthalenedisulfonate.The phase transition behaviors were studied by differential scanning calorimetry,single-crystal X-ray analysis and dielectric measurements.Compounds 1 and 2 undergo phase transitions at about 202 and 148 K,respectively,accompanied by distinct dielectric changes.The origin of the phase transitions was ascribed to motional changes of the cations in the crystal lattices.
基金supported by the National Science&Technology Major Project[grant No.2017ZX100203205]National Natural Science Foundation of China[grant No.81930119]funders had no role in design of the study and collection,analysis,and interpretation of data and in writing the manuscript.
文摘Up to know,product information for COVID-19 mRNA vaccines do not include their use in patients receiving immunosuppressive medication,since they were not included in trials(1).For the BNT162b2 mRNA vaccine,one case of otherwise totally asymptomatic elevations of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)andγ-glutamyl transpeptidase(GGT)serum concentrations in a liver transplant recipient has been reported after the first injection(2).In the present retrospective study,we present safety outcomes of COVID-19 vaccinations in 56 liver transplant patients from two Chinese centers vaccinated with the domestic PiCoVacc,BBIBP-CorV and ZF2001 vaccines.Thirty vaccinated physicians from the Hepatopancreatobiliary Center of Beijing Tsinghua Changgung Hospital served as control,from which 1 participant received an Ad5-nCoV vaccine.The study deadline was 2021-10-31.The observation period for adverse reactions,such as muscle pain and fatigue was 1 week,while for laboratory examinations such as liver function the follow-up period was 1 month.