SIRT2 interacts with DDX24 to promote nasopharyngeal carcinoma growth

Background:Nasopharyngeal carcinoma(NPC)is one of the most prevalent cancers in Southeast Asia.Sirtuin 2(SIRT2)is a member of the NAD+-dependent deacetylase family and has been shown to play important roles in numerous biological processes.However,Its function in NPC remains uncertain.The primary aim of this study is to clarify the role of SIRT2 in NPC.Methods:In this research,we examined the effect of SIRT2 silencing on NPC cell proliferation and colony formation using vitro NPC cell lines.Co-immunoprecipitation and mass spectrometry was applied to identify SIRT2-interacting proteins in NPC cells.Results:In comparison to nasopharyngeal epithelial NP69 cells,SIRT2 was up-regulated in multiple NPC cell lines,particularly in CNE2 cells.SIRT2 knockdown abrogated CNE2 cell proliferation and colony formation,whereas SIRT2 overexpression promoted HNE1 cell proliferation and colony formation.The SIRT2-interacting proteins were gathered in gene expression and regulation processes including RNA processing and translation.Among the SIRT2-interacting proteins,there were multiple DEAD-box(DDX)family members.Of note,silencing of DDX24 phenocopied the effect of SIRT2 knockdown on NPC growth.Overexpression of DDX24 restored SIRT2-depleted CNE2 cells to proliferative and colony formation.Conclusions:Our study indicates that SIRT2 can interact with DDX24 to enhance NPC growth.The clinical relevance of SIRT2 and DDX24 in NPC warrants further investigation.

基于立体化教学体系的高等教育复合型人才培养和课程建设——以基础医学为例

该研究以人体寄生虫学教学为例,在教学内容整合的基础上,尝试了"参与式课堂+现场实践""线下+线上互动"的立体化教学范式。新模式不仅是课程结构和教学方法的改革,也促进了师资和学科建设,为医学院校在专业基础教育阶段培养复合创新型人才提供了新的模式和经验。

Cell Proliferation Inhibition by Sericin from the Wild Silkworm, Cricula trifenestrata

Sericin is a major silk protein, and recent studies have shown that sericin has numerous physiological activities against cultured cells. For example, it accelerates mammalian cell proliferation and protects insect cells against cell death. In this study, we examined the activities of sericin prepared from the wild silkworm, Cricula trifenestrata. The results presented here indicated that C. trifenestrata sericin has anti-proliferative activity against feline kidney cells, while sericin from Bombyx mori promoted cell proliferation. The components of the sericin preparation that played a role in inhibition of proliferation were analyzed by fractionation through partition chromatography (Folch method) and reversed-phase chromatography. Our analysis showed that at least two components in the C. trifenestrata sericin preparation promoted the anti-proliferative activity in an additive or even synergistic manner. Therefore, differences in components between insect species may contribute to different activities against cultured cells.

The NCC: An Improved Anonymous Method for Location-Based Services Based on Casper

Casper Cloak is a privacy protection method based on K-anonymity algorithm. To be anonymous, Casper Cloak needs to search regional sibling and parent node, which requires a complex process and huge expenditure. In addition,the anonymous area has space redundancy and it is not accurate enough to achieve high Location-Based Services (LBS) quality. To address these problems,this paper proposes an improved privacy protection method-NCC, based on the Casper Cloak. To reduce the unnecessary search, NCC introduced the concept of the first sibling node. NCC also improves the LBS quality by considering the characteristics of user mobility. Moreover, the improved method,NCC, which is incorporated with a redundancy optimization processing strategy,realizing more precise in the anonymous area and accurately guaranteeing the related degree of privacy. Adopting NCC verification experiments reflects varied advantages as bellow: (1) By reducing 80% searching time, NCC highly improved searching process. (2) The anonymous area produced in NCC not only meet users' anonymous demands, but the direction of the mobility which improves 4 times accuracy of services in comparison with Casper mode.(3) According to optimization strategy, NCC can reach minimum anonymous area index, increasing the rates of anonymous optimization in original algorithm.

基于岛–桥结构的拉伸AMOLED显示技术

LTPS–AMOLED技术已经广泛应用在柔性显示领域,但是由于脆性膜层材料限制无法直接应用于拉伸显示领域.本文以LTPS–AMOLED技术为基础结合镂空技术制备了具有岛桥结构的拉伸显示器件.为确保拉伸显示具有良好的拉伸性能,本文研究了影响拉伸性能的影响因素.首先通过研究拉伸变形机理发现拉伸结构设计时减小桥宽度、增加桥的长度、减小夹角角度和适当增大夹角半径可以更好的释放应力应变.其次研究表明拉伸过程中连接桥会产生拉应变、压应变和中性位置,金属引线位于中性位置或者压应变位置会有效减小断裂风险.连接桥的无机层裂纹产生的诱导断裂会随着其膜厚的增加而增强,而有机层可以阻隔诱导断裂并且阻隔性随着有机层厚度增加而增强,因此通过减小连接桥的无机层厚度、增加有机层厚度可有效减小无机层断裂产生的影响.最后制备了2.3 inch的OLED可伸缩显示屏,水平方向的拉伸量可达到5%以上.

Gold nanorods core/AgPt alloy nanodots shell： A novel potent antibacterial nanostructure

根据银 nanoparticles (Ag NP ) 的当前的问题以抗菌剂性能，我们与取向附生地作为一个潜在的抗菌剂代理人在金 nanorods (Au@PtAg NR ) 上种的 AgPt 合金 nanodots 设计了新奇 trimetallic 核心 / 壳 nanostructure。两 Escherichia coli (E。coli ) 并且葡萄球菌 aureus (S。aureus ) 被学习。抗菌剂活动展出明显的作文依赖。在在合金壳增加 Ag 部分直到 80% 以后，抗菌剂活动逐渐地增加，表明一个灵活方法调节这项活动。在 80% Ag，抗菌剂活动比纯 Ag 壳的好。改进抗菌剂能力主要由于点形态学在壳表面上源于银的高暴露。当为基于 Ag 的 nanomaterials 保留高化学的稳定性时，我们因此证明形成合金是改进抗菌剂活动的一个有效方法。而且，由于悦耳的局部性的表面 plasmonic 反应在在红外线附近(NIR ) 光谱区域，对把 lightsuch 用作杀死的 photothermal 和 phototriggered 银离子 releaseis 的抗菌剂活动的另外的控制期望。作为示范，高度提高了抗菌剂活动被利用 nanostructures 的 NIR photothermal 效果显示出。我们的结果显示如此的定制的 nanostructures 将在对细菌的未来战斗发现一个角色，包括 multidrug 抵抗的增加的严厉的挑战。

Fabrication of chiral plasmonic oligomers using cysteine-modified gold nanorods as monomers

在紫外区域以外的圆形的二色性(CD ) 的产生具有对开发 chiral 传感器和 chiroptical 设备的大兴趣。此处，我们在可见、近的红外与强壮的 CD 反应为 plasmonic oligomers 的制造表明一个简单、万用的方法光谱范围。oligomers 被被触发制作一起修改半胱氨酸的金 nanorods 的汇编。修改 nanorods 自己没展出明显的 plasmonic CD 信号；然而， oligomers 表演在电浆子回声波长附近的强壮的 CD 乐队。CD 乐队的符号被吸收半胱氨酸分子的 chirality 支配。由调整 oligomers， chiral 分子的集中，或 nanorods 的方面比率的尺寸， CD 紧张并且光谱范围是容易地悦耳的。理论计算建议 oligomers 的那 CD 在 oligomer 以内从邻近的 nanorods 的细微扭曲发源。因此，我们建议吸附的 chiral 分子能操作在 nanorods 之间的扭曲角度并且因此调制 oligomers 的 CD 反应。

Ferroxidase-like activity of Au nanorod/Pt nanodot structures and implications for cellular oxidative stress

铂 nanoparticles (NP ) 被报导模仿各种各样的抗氧化剂酶并且可以因此由减少反应的氧生产积极生物效果种类(ROS ) 层次。在这张手稿，我们报导磅 NP 由在金 nanorods (Au@Pt NDR ) 上扔铂 nanodots ferroxidase 作为酶模仿。Au@Pt NDR 显示出 pH 依赖的象 ferroxidase 一样活动并且在中立 pH 价值举办更高的活动。有人的房间线(肺腺癌 A549 和正常支气管的上皮的房间线 HBE ) 的 Cytotoxicity 结果证明 Au@Pt NDR 经由 endocytosis 和 translocate 被收起进房间进 endosome/lysosome。Au@Pt NDR 在比 0.15 n 低的 NDR 粒子集中有好 biocompatibility。然而，面对 H 2 O 2, lysosomelocated NDR 展出象 peroxidase 一样活动因此增加 cytotoxicity。面对 Fe 2+, ， NDR 的象 ferroxidase 一样活动由消费 H 2 O 2 。当在生物系统采用 Au@Pt NDR 时，彻底的考虑应该被给这行为。

Rod-shaped Au@PtCu nanostructures with enhanced peroxidase-like activity and their ELISA application

Pt and its based alloy nanoparticles(NPs)have been reported to demonstrate novel enzyme-like activities.Varying composition is very important to realize the optimization of their functions through the tuning of electronic structure.In this paper,our effort is focused in this direction by tailoring the electronic structure of Pt NPs via alloying with copper.Using gold nanorod(Au NR)as core,a simple method to prepare PtCu alloy shell is developed(termed as Au@PtCu NR).The introduction of copper could result in endcap-preferred growth mode owing to the lattice mismatch between alloy shell and the Au core.The variation in the electronic structure changes the substrate affinity,and enhanced affinity was found for H2O2.Besides,the designed Au@PtCu nanostructures have realized spatial separation of catalytic and recognition sites.Binding of recognition antibodies had negligible effect on their catalytic activity.Based on their peroxidaselike activity,a highly sensitive detection of human immunoglobulin G(IgG)was demonstrated in a direct enzyme-linked immunosorbent assay(ELISA)mode.The detection limit can be as low as 90 pg/mL.

Anthraquinone acted as a catalyst for the removal of triphenylmethane dye containing tertiary amino group: Characteristics and mechanism

Herein,we found that anthraquinone(AQ)acted as a catalyst for the rapid and effective removal of triphenylmethane dye containing tertiary amino group(TDAG).Results showed that AQ had an enhanced catalytic reactivity towards the removal of TDAG compared to hydro-quinone,which was further proved and explained using density functional theory(DFT)calculations.AQs could achieve a TDAG removal efficiency and rate of approximately 100%and 0.3583 min^(−1),respectively,within 20 min.Quenching experiments and electron paramagnetic resonance(EPR)tests indicated that the superoxide radical(O_(2)^(•−))generated through the catalytic reduction of an oxygen molecule(O_(2))by AQ contributed to the effective removal of the TDAG.In addition,itwas found that the electrophilic attack of the O_(2)^(•−)radical on the TDAG was the driving force for the dye degradation process.Decreasing the pH led to protonation of the substituted group of AG,which resulted in formation of an electron deficient center in the TDAGmolecule(TDAG-EDC+)through delocalization of the π electron.Therefore,the possibility of the electrophilic attack for the dye by the negative O_(2)^(•-)radical was significantly enhanced.This study revealed that the H+and the O_(2)^(•−)generated by the catalytic reduction of O_(2) have synergistic effects that led to a significant increase in the dye removal rate and efficiency,which were higher than those obtained through persulfate oxidation.

Study of the shear-rate dependence of granular friction based on community detection

In this study, computer simulations are performed on three-dimensional granular systems under shear conditions. The system comprises granular particles that are confined between two rigid plates. The top plate is subjected to a normal force and driven by a shearing velocity. A positive shear-rate dependence of granular friction, known as velocity-strengthening, exists between the granular and shearing plate. To understand the origin of the dependence of frictional sliding, we treat the granular system as a complex network, where granular particles are nodes and normal contact forces are weighted edges used to obtain insight into the interiors of granular matter. Community structures within granular property networks are detected under different shearing velocities in the steady state. Community parameters, such as the size of the largest cluster and average size of clusters, show significant monotonous trends in shearing velocity associated with the shear-rate dependence of granular friction. Then, we apply an instantaneous change in shearing velocity. A dramatic increase in friction is observed with a change in shearing velocity in the non-steady state. The community structures in the non-steady state are different from those in the steady state. Results indicate that the largest cluster is a key factor affecting the friction between the granular and shearing plate.

Agent model for multi-UAV control via protocol designs

Purpose–Cooperative control of a group of unmanned aerial vehicles(UAVs)is an important area of research.The purpose of this paper is to explore multi-UAV control in the framework of providing surveillance of areas of interest with automatic loss detection and replacement capabilities.Design/methodology/approach–The research is based on the concept of the multi-agent system.The authors present the framework of the multi-agent and protocol design for monitoring the network of a group of UAVs.Findings–If one or more UAVs which is conducting a high priority surveillance task is lost,the system can self-arrange for another UAV to replace the lost UAV and continue to execute its task.This research provides an excellent design protocol for UAV loss detection and replacement scheme.Research limitations/implications–One of the major limitations of this research is that we have only two types of priority levels,high or low.If the priority is more than two levels,for example,high priority 1,high priority 2,or high priority 3,the replacement has not yet been implemented.Originality/value–This paper contributes to the following two aspects of the scientific knowledge.The first contribution is the design of an agent model which jointly considers system architecture,communication,control logic and target monitoring.The second contribution includes the decentralized and automatic UAV loss detection and replacement algorithm.

The roadmap of interest rate liberalisation in China

This paper examines the roadmap of interest rate liberalisation in China,including the current dual-track interest rate system and the future benchmark rate system.It provides a theoretical foundation for China to develop its own benchmark interest rate.A vector autoregression model is estimated to investigate the effectiveness of Chinese market interest rates,Shanghai Interbank Offered Rate(SHIBOR),and repo rates against different factors such as market size,volatility,transmission channels of monetary policy,and term structures of interest rates.The result shows that SHIBOR affects both the market and the economy.As SHIBOR promptly reflects the changes in currency markets,we argue that it has the potential to become China’s benchmark interest rate.

Whither Second-Sphere Coordination?

The properties of coordination complexes are dictated by both the metals and the ligands.The use of molecular receptors as second-sphere ligands enables significant modulation of the chemical and physical properties of coordination complexes.In this minireview,we highlight recent advances in functional systems based on molecular receptors as second-sphere coordination ligands,as applied in molecular recognition,synthesis of mechanically interlocked molecules,separation of metals,catalysis,and biomolecular chemistry.These functional systems demonstrate that second-sphere coordination is an emerging and very promising strategy for addressing societal challenges in health,energy,and the environment.