Characteristics of rural domestic sewage discharge and their driving mechanisms: evidence from the Northern Region, China

The traits of rural domestic sewage emission are unclear,negatively affecting rural domestic sewage treatment and sewage management.This study used data from the Second National Pollution Source Census Bulletin to establish a data set.The spatial distribution characteristics and main factors influencing rural sewage discharge in the Northern Region were studied using spatial autocorrelation analysis and structural equations.The findings demonstrated that(l)a significant Spearman correlation between drainage water volume(DwV),chemical oxygen demand(COD),ammonia nitrogen(NH_(3)-N),total nitrogen(TN),and total phosphorus(TP)and that the correlation coefficients between DWV and COD,NH,-N,TNand TP were 0.87**,1.0**,0.99**,0.99**,respectively;(2)rural sewage discharge showed spatial autocorrelation,and rural domestic sewage discharge in the districts and counties with an administration was significantly higher than in the surrounding areas;and(3)social development was the main driver rural domestic sewage changes(path coefficient was 0.407**),and the main factors influencing rural domestic sewage discharge were the urbanization rate,years of education,and population age structure.This study obtained the spatial variation law and clarified the main influencing factors of rural domestic sewage to provide data support and a theoretical basis for subsequent rural sewage collection and treatment.Use of the Inner Mongolia Autonomous Region in northern China as a typical case,provides a theoretical foundation for scientific decision-making on rural domestic sewage treatment at the national and regional levels and offers new perspectives for managing pollutants.

Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity

Interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for T-cell immune responses remains poorly understood. Using a Listeria monocytogenes （Lm） infection model, we demonstrate that dendritic cells （DCs） require the support of macrophages to elicit protective immunity against Lm infection. DCs themselves were inefficient at taking up Lm but capable of taking up microparticles （MPs） released by Lm-infected macrophages. These MPs transferred Lm antigens to DCs, allowing DCs to present Lm antigen to effector T cells. MP-mediated Lm antigen transfer required M HC class I participation, since M HC class I deficiency in macrophages resulted in a significant reduction of T-cell activation. Moreover, the vaccination of mice with MPs from Lm-infected macrophages produced strong protective immunity against Lm infection. We here identify an intrinsic antigen transfer program between macrophages and DCs during Lm infection, and emphasize that macrophages also play an essential role in DC-elicited Lm-specific T-cell responses.

Assessment of river ecosystem health in Tianjin City,China:index of ecological integrity and water comprehensive pollution approach

Evaluation of the river ecological environment can provide a basis for river management and ecological restoration.To conduct a comprehensive health assessment of the rivers in Tianjin,their biological,physical,and chemical indicators are investigated on the basis of 32 river monitoring sites from August to September 2018.The comprehensive pollution and ecological integrity indexes of the rivers are analyzed.Results of the two evaluations,compared to achieve the river ecological environment evaluation,are as follows.1)Index of Ecological Integrity evaluation shows that among the sampling points,18.8%are"healthy",28.1%are"sub-healthy",40.6%are"fair",6.3%are"poor",and 6.3%are"very poor".2)The comprehensive evaluation of the chemical properties of the 32 river ecosystems in Tianjin shows severe overall river pollution and low standard water function area.Of the total sampling sites,16(50%)are heavily contaminated and 10(31.3%)are moderately contaminated.Excessive chemical oxygen demand and ammonia nitrogen are the main causes of water pollution.3)The Index of Ecological Integrity(IEI)has high correspondence with environmental factors.Pearson correlation analysis results show that the IEI index is significantly correlated with permanganate index(R=-0.453;P=0.023<0.05).Analysis results using BEST show that ammonia nitrogen is the best environmental parameter to explain the changes in IEI(R_(ho)=0.154;P=0.02<0.05)and those using RELATE show significant correlation between the biotic index and the environmental parameter matrices(R_(ho)=0.154;P=0.034<0.05).