Young moso bamboo shoots are a popular seasonal food and an important source of income for farmers,with value for cultivation estimated at$30,000 per hectare.Bamboo also has great environmental importance and its uniq...Young moso bamboo shoots are a popular seasonal food and an important source of income for farmers,with value for cultivation estimated at$30,000 per hectare.Bamboo also has great environmental importance and its unique physiology is of scientific interest.A rare and valuable phenomenon has recently appeared where a large number of adjacent buds within a single moso bamboo rhizome have grown into shoots.Although of practical importance for the production of edible shoots,such occurrences have not been scientifically studied,due to their rarity.Analysis of collected reports from enhanced shoot production events in China showed no evidence that enhanced shoot development was heritable.We report the analysis of the rhizosphere microbiome from a rhizome with 18 shoots,compared to rhizomes having one or no shoots as controls.The community of prokaryotes,but not fungi,correlated with the shoot number.Burkholderia was the most abundant genus,which was negatively correlated with rhizome shoot number,while Clostridia and Ktedonobacteria were positively correlated.Two Burkholderia strains were isolated and their plant-growth promoting activity was tested.The isolated Burkholderia strains attenuated the growth of bamboo seedlings.These data provide the first study on excessive shoot development in bamboo,which will facilitate hypothesis building for future studies.展开更多
Enzymatic malonylation of natural glycosides provides a promising alternative method for drug-like malonylated glycosides supply.However,the catalytic potential and structural basis of plant malonyltransferase are far...Enzymatic malonylation of natural glycosides provides a promising alternative method for drug-like malonylated glycosides supply.However,the catalytic potential and structural basis of plant malonyltransferase are far from being fully elucidated.This work identified a new malonyltransferase CtMaT1 from Cistanche tubulosa.It displayed unprecedented mono-and/or di-malonylation activity toward diverse glucosides with different aglycons.A“one-pot”system by CtMaT1 and a malonyl-CoA synthetase was established to biosynthesize nine new malonylated glucosides.Structural investigations revealed that CtMaT1 possesses an adequately spacious acyl-acceptor pocket capable of accommodating diverse glucosides.Additionally,it recognizes malonyl-CoA through strong electrotactic and hydrogen interactions.QM/MM calculation revealed the H167-mediated SN2 reaction mechanism of CtMaT1,while dynamic simulations detected the formation of stable hydrogen bonds between the glucose-6-OH group and H167,resulting in its high malonylation regiospecificity.Calculated energy profiles of two isomeric glycosides highlighted lower reaction energy barriers towards glucoside substrates,emphasizing CtMaT1's preference for glucosides.Furthermore,a mutant CtMaT1H36A with notably increased di-malonylation activity was obtained.The underlying molecular mechanism was illuminated through MM/GBSA binding free energy calculation.This study significantly advances the understanding of plant acyltransferases from both functional and protein structural perspectives,while also providing a versatile tool for enzymatic malonylation applications in pharmacology.展开更多
In this paper,an Unmanned Aerial Vehicle(UAV)enabled Mobile Edge Computing(MEC)system is studied,in which UAV acts as server to offer computing offloading service to the Mobile Users(MUs)with limited computing capabil...In this paper,an Unmanned Aerial Vehicle(UAV)enabled Mobile Edge Computing(MEC)system is studied,in which UAV acts as server to offer computing offloading service to the Mobile Users(MUs)with limited computing capability and energy budget.We aim to minimize the total energy consumption of MUs by jointly optimizing the bit allocation for uplink,computing at the UAV and downlink,along with the UAV trajectory in a unified framework.To this end,a trajectory constraint model is employed to avoid sudden changes of velocity and acceleration during flying.Due to high-order information in use,we lead to a more reasonable nonconvex optimization problem than prior arts.An Alternating Direction Method of Multipliers(ADMM)method is introduced to solve the optimization problem,which is decomposed into a set of easy subproblems,to meet the requirement on the efficiency in edge computing.Numerical results demonstrate that our approach leads a smoother UAV trajectory,significantly save the energy consumption for UAV during flying.展开更多
基金supported by the Qianjiang Talent D program to FCthe National Natural Science Foundation of China(grant no.31700224+4 种基金3187123331770543)the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(grant no.2016C02056-1)the Program for Changjiang Scholars and Innovative Research Team in University(grant no.IRT_17R99 to SL)funded by the Academy of Finland Center of Excellence in Primary Producers 2014-2019(decisions#271832 and 307335).
文摘Young moso bamboo shoots are a popular seasonal food and an important source of income for farmers,with value for cultivation estimated at$30,000 per hectare.Bamboo also has great environmental importance and its unique physiology is of scientific interest.A rare and valuable phenomenon has recently appeared where a large number of adjacent buds within a single moso bamboo rhizome have grown into shoots.Although of practical importance for the production of edible shoots,such occurrences have not been scientifically studied,due to their rarity.Analysis of collected reports from enhanced shoot production events in China showed no evidence that enhanced shoot development was heritable.We report the analysis of the rhizosphere microbiome from a rhizome with 18 shoots,compared to rhizomes having one or no shoots as controls.The community of prokaryotes,but not fungi,correlated with the shoot number.Burkholderia was the most abundant genus,which was negatively correlated with rhizome shoot number,while Clostridia and Ktedonobacteria were positively correlated.Two Burkholderia strains were isolated and their plant-growth promoting activity was tested.The isolated Burkholderia strains attenuated the growth of bamboo seedlings.These data provide the first study on excessive shoot development in bamboo,which will facilitate hypothesis building for future studies.
基金This work was financially supported by National Key Research and Development Program Special Project of Synthetic Biology(Grant No.2023YFA0914100/2023YFA0914103)National Natural Science Foundation of China(Grant No.82173922,81402809)+3 种基金Beijing Natural Science Foundation(Grant No.7192112)Fundamental Research Funds for the Central Universities(Grant No.2023-JYB-JBQN-054,China)Young Elite Scientists Sponsorship Program by CAST(Grant No.CACM-2018-QNRC1-02,China)State Key Laboratory of Natural and Biomimetic Drugs Foundation(Grant No.K202119,China).
文摘Enzymatic malonylation of natural glycosides provides a promising alternative method for drug-like malonylated glycosides supply.However,the catalytic potential and structural basis of plant malonyltransferase are far from being fully elucidated.This work identified a new malonyltransferase CtMaT1 from Cistanche tubulosa.It displayed unprecedented mono-and/or di-malonylation activity toward diverse glucosides with different aglycons.A“one-pot”system by CtMaT1 and a malonyl-CoA synthetase was established to biosynthesize nine new malonylated glucosides.Structural investigations revealed that CtMaT1 possesses an adequately spacious acyl-acceptor pocket capable of accommodating diverse glucosides.Additionally,it recognizes malonyl-CoA through strong electrotactic and hydrogen interactions.QM/MM calculation revealed the H167-mediated SN2 reaction mechanism of CtMaT1,while dynamic simulations detected the formation of stable hydrogen bonds between the glucose-6-OH group and H167,resulting in its high malonylation regiospecificity.Calculated energy profiles of two isomeric glycosides highlighted lower reaction energy barriers towards glucoside substrates,emphasizing CtMaT1's preference for glucosides.Furthermore,a mutant CtMaT1H36A with notably increased di-malonylation activity was obtained.The underlying molecular mechanism was illuminated through MM/GBSA binding free energy calculation.This study significantly advances the understanding of plant acyltransferases from both functional and protein structural perspectives,while also providing a versatile tool for enzymatic malonylation applications in pharmacology.
基金the Defense Industrial Technology Development Program of China(No.JCKY2017601C006)the National Key Research and Development Program of China(No.2016YFB0502602)+1 种基金the National Natural Science Foundation of China(No.91538204)in part supported by Shenzhen Science and Technology Program,China(No.KQTD2016112515134654)。
文摘In this paper,an Unmanned Aerial Vehicle(UAV)enabled Mobile Edge Computing(MEC)system is studied,in which UAV acts as server to offer computing offloading service to the Mobile Users(MUs)with limited computing capability and energy budget.We aim to minimize the total energy consumption of MUs by jointly optimizing the bit allocation for uplink,computing at the UAV and downlink,along with the UAV trajectory in a unified framework.To this end,a trajectory constraint model is employed to avoid sudden changes of velocity and acceleration during flying.Due to high-order information in use,we lead to a more reasonable nonconvex optimization problem than prior arts.An Alternating Direction Method of Multipliers(ADMM)method is introduced to solve the optimization problem,which is decomposed into a set of easy subproblems,to meet the requirement on the efficiency in edge computing.Numerical results demonstrate that our approach leads a smoother UAV trajectory,significantly save the energy consumption for UAV during flying.
