We attempt to determine the effect of the dietary switch from a native to non-native prey on the gut microbiota in the predaceous ladybird Harmonia axyridis larvae and adults and examine how the dietary effect may var...We attempt to determine the effect of the dietary switch from a native to non-native prey on the gut microbiota in the predaceous ladybird Harmonia axyridis larvae and adults and examine how the dietary effect may vary across generations.We fed H.axyridis with different diets,native aphid Megoura japonica(Matsumura)versus non-native mealy-bug Phenacoccus solenopsis(Tinsley),for 5 generations and sequenced microbes in the gut of the 3rd instar larvae and adults of the Ist,3rd,and 5th generations.In addition,we identified microbes in M.japonica and P solenopsis.The 2 prey species differed in microbial community as measured by abundances of prevalent microbial genera and diver-sity.In H.axyridis,abundances of some prevalent microbial genera differed between the 2 diets in the 1st and 3rd generations,but the difference disappeared in the 5th generation;this tendency is more obvious in adults than in larvae.Overall,gut microbial assemblages became gradually cohesive over generations.Microbial diversity differed between diets in the 1st and 3rd generations but became similar in the 5th generation.Major prevalent gut microbial genera are predicted to be associated with metabolic functions of H.axyridis and associated genera are more abundant for consuming the mealybug than the aphid.Our findings from this study suggest that the gut microbiota in H.axyridis is flexible in re-sponse to the dietary switch,but tends toward homogeneity in microbial composition over generations.展开更多
基金This research was funded by the National Key R&D Program of China(2017YFE0104900)awarded to Ling Meng.
文摘We attempt to determine the effect of the dietary switch from a native to non-native prey on the gut microbiota in the predaceous ladybird Harmonia axyridis larvae and adults and examine how the dietary effect may vary across generations.We fed H.axyridis with different diets,native aphid Megoura japonica(Matsumura)versus non-native mealy-bug Phenacoccus solenopsis(Tinsley),for 5 generations and sequenced microbes in the gut of the 3rd instar larvae and adults of the Ist,3rd,and 5th generations.In addition,we identified microbes in M.japonica and P solenopsis.The 2 prey species differed in microbial community as measured by abundances of prevalent microbial genera and diver-sity.In H.axyridis,abundances of some prevalent microbial genera differed between the 2 diets in the 1st and 3rd generations,but the difference disappeared in the 5th generation;this tendency is more obvious in adults than in larvae.Overall,gut microbial assemblages became gradually cohesive over generations.Microbial diversity differed between diets in the 1st and 3rd generations but became similar in the 5th generation.Major prevalent gut microbial genera are predicted to be associated with metabolic functions of H.axyridis and associated genera are more abundant for consuming the mealybug than the aphid.Our findings from this study suggest that the gut microbiota in H.axyridis is flexible in re-sponse to the dietary switch,but tends toward homogeneity in microbial composition over generations.
