β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ...β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ratiometric fluorescent probe has been successfully synthesized for detecting the β-galactosidase in living cells.The as-prepared probe exhibits two emission peaks at 490 nm and 530 nm,respectively,and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β-galactosidase.This present ratiometric fluorescent probe is,therefore,very promising for effective,sensitive,and selective detection of the β-galactosidase in living cells.展开更多
基金National Key R&D Program of China(2016YFA0200800)NSFC(51672284)+1 种基金Beijing Natural Science Foundation(2181002)Chinese Academy of Sciences(1A1111KYSB20180017,QYZDJ-SSW-JSC032,XDB17000000).
文摘β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ratiometric fluorescent probe has been successfully synthesized for detecting the β-galactosidase in living cells.The as-prepared probe exhibits two emission peaks at 490 nm and 530 nm,respectively,and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β-galactosidase.This present ratiometric fluorescent probe is,therefore,very promising for effective,sensitive,and selective detection of the β-galactosidase in living cells.
