Doxorubicin(DOX) is widely used in cancer therapy. However, its application is sometimes limited by its adverse cardiotoxicity and delivery pathways. In our study, we prepared a topical implantable delivery device for...Doxorubicin(DOX) is widely used in cancer therapy. However, its application is sometimes limited by its adverse cardiotoxicity and delivery pathways. In our study, we prepared a topical implantable delivery device for controlled drug release and site-specific treatment. The core region consisted of poly(lactic co-glycolic acid) and poly-caprolactone, whereas the shell region was composed of cross-linked gelatin.DOX was enclosed in the core region of a core-shell nanofiber obtained by electrospinning. This implantable delivery device was implanted on the top of the melanoma in a mouse model, which had shown a DOX-controlled release profile with sustained and sufficient local concentration against melanoma growth in mice with negligible side effects. Compared with the traditional intravenous administration,the implantable device allows precisely localized treatment and therefore can reduce the dose, decrease the injection frequency, and ensure antitumor efficacy associated with lower side effects to normal tissues. Using a coaxial electrospinning process, it is promising to deliver different hydrophobic or hydrophilic drugs for direct tumor site-specific therapy without large systemic doses and minimized systemic toxicity.展开更多
Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）...Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）（OH）n, acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C（60）（OH）n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca（2＋） along with the apoptotic cell death. Two FDAapproved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers,with C（60）（OH）n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging.展开更多
基金supported by the Project Electro Med (11115313) from the Danish Council for Strategic Researchthe National Science Fund for Excellent Young Scholars (31622026)+3 种基金the National Natural Science Foundation of China (U1532122, 21320102003, 21471044)the National Key Research and Development Plan (2016YFA0201600, 2016YFA0203204)the National Science Fund for Distinguished Young Scholars (11425520)Youth Innovation Promotion Association of the Chinese Academy of Sciences (2014031)
文摘Doxorubicin(DOX) is widely used in cancer therapy. However, its application is sometimes limited by its adverse cardiotoxicity and delivery pathways. In our study, we prepared a topical implantable delivery device for controlled drug release and site-specific treatment. The core region consisted of poly(lactic co-glycolic acid) and poly-caprolactone, whereas the shell region was composed of cross-linked gelatin.DOX was enclosed in the core region of a core-shell nanofiber obtained by electrospinning. This implantable delivery device was implanted on the top of the melanoma in a mouse model, which had shown a DOX-controlled release profile with sustained and sufficient local concentration against melanoma growth in mice with negligible side effects. Compared with the traditional intravenous administration,the implantable device allows precisely localized treatment and therefore can reduce the dose, decrease the injection frequency, and ensure antitumor efficacy associated with lower side effects to normal tissues. Using a coaxial electrospinning process, it is promising to deliver different hydrophobic or hydrophilic drugs for direct tumor site-specific therapy without large systemic doses and minimized systemic toxicity.
基金financially supported by the project Electro Med(No.11-115313) from the Danish Council for Strategic Researchthe National Science Fund for Excellent Young Scholars(No.31622026)+2 种基金the National Natural Science Foundation of China(Nos.U1532122,11435002,21471044)the National Key Research and Development Plan(Nos.2016YFA0201600,2016YFA0203204)CAS Youth Innovation Promotion Association(No.2014031)
文摘Environmental stress factors could lead to the excess generation of reactive oxygen species（ROS） that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C（60）（OH）n, acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C（60）（OH）n loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca（2＋） along with the apoptotic cell death. Two FDAapproved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers,with C（60）（OH）n added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging.
