This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as subs...This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.展开更多
In order to improve the yield of β-mannase and to investigate the rules of fermentation production, a high-yield β-mannase producing strain, Bacillus licheniformis HDYM-04, was used to investigate the kinetics model...In order to improve the yield of β-mannase and to investigate the rules of fermentation production, a high-yield β-mannase producing strain, Bacillus licheniformis HDYM-04, was used to investigate the kinetics models based on the optimal fermentation conditions: HDYM-04 strain was fermented at 37℃ for 30 h with agitation speed at 300 r/min and aeration rate at 3 L/min in a 5 L fermenter, the initial addition amount of konjac flour was 2%(w/v), the initial pH of medium was 8.0, and the inoculum concentration was 6.7%(v/v). Three batch fermentation kinetic models were established(cell growth kinetic model, substrate consumption kinetic model,product formation kinetic model) bases on Logistic and Luedeking-Piret equations.To be specific, cell growth kinetic model wasdX dt=0.431X(1-X15.522), substrate consumption kinetic model was-dS dt=1.11dX dt+0.000 2dP dt+0.000 8X, and product formation kinetic model wasdP dt=133.1dX dt+222.87X. The correlation coefficients R2of the three equations were 0.990 21, 0.989 08 and 0.988 12, respectively, which indicated a good correlation between experimental values and models. Therefore,the three equations could be used to describe the processes of cell growth, enzyme synthesis and substrate consumption during batch fermentation using B. licheniformis strain HDYM-04. The establishment of batch fermentation kinetic models(cell growth kinetic model, substrate depletion kinetic model, product formation kinetic model)could lay the theoretical foundation and provide practical reference for the application of HDYM-04 in fermentation industry.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 31270534, 31770538)the National Natural Science Youth Foundation of China (No. 31300355)the Post-Doctorate Foundation of Heilongjiang Province (No. LBH-Z15214)
文摘This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.
基金Supported by Educational Commission of Heilongjiang Province of China(11551z011)
文摘In order to improve the yield of β-mannase and to investigate the rules of fermentation production, a high-yield β-mannase producing strain, Bacillus licheniformis HDYM-04, was used to investigate the kinetics models based on the optimal fermentation conditions: HDYM-04 strain was fermented at 37℃ for 30 h with agitation speed at 300 r/min and aeration rate at 3 L/min in a 5 L fermenter, the initial addition amount of konjac flour was 2%(w/v), the initial pH of medium was 8.0, and the inoculum concentration was 6.7%(v/v). Three batch fermentation kinetic models were established(cell growth kinetic model, substrate consumption kinetic model,product formation kinetic model) bases on Logistic and Luedeking-Piret equations.To be specific, cell growth kinetic model wasdX dt=0.431X(1-X15.522), substrate consumption kinetic model was-dS dt=1.11dX dt+0.000 2dP dt+0.000 8X, and product formation kinetic model wasdP dt=133.1dX dt+222.87X. The correlation coefficients R2of the three equations were 0.990 21, 0.989 08 and 0.988 12, respectively, which indicated a good correlation between experimental values and models. Therefore,the three equations could be used to describe the processes of cell growth, enzyme synthesis and substrate consumption during batch fermentation using B. licheniformis strain HDYM-04. The establishment of batch fermentation kinetic models(cell growth kinetic model, substrate depletion kinetic model, product formation kinetic model)could lay the theoretical foundation and provide practical reference for the application of HDYM-04 in fermentation industry.
