Objective: Cancer vaccines that rely on tumor antigen-specific CD8^+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite(G...Objective: Cancer vaccines that rely on tumor antigen-specific CD8^+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite(GAS) could be used as a novel vector to induce antigen-specific CD8^+ T cell responses against lung cancer.Methods: We constructed GAS/MAGE-A3, a recombinant GAS engineered to express the lung cancer-specific antigen, melanomaassociated antigen 3(MAGE-A3), and assessed its therapeutic effects against lung cancer.Results: Robust parasite-specific CD8αlow CD11ahigh and CD49dhigh CD11ahigh CD4^+ T cell responses as well as a MAGE-A3-specific CD8^+ T cell response were induced in GAS/MAGE-A3-immunized mice. Adoptive transfer of GAS/MAGE-A3-induced CD8^+ T cells from HLA-A2 transgenic mice into lung cancer-bearing nude mice inhibited tumor growth and prolonged survival.Conclusions: These findings demonstrate that GAS/MAGE-A3 induces a strong MAGE-A3-specific CD8^+ T cell response against lung cancer in vivo, and indicate that GAS is a novel and efficacious antigen delivery vector for antitumor immunotherapy.展开更多
Summary What is already known about this topic?Research evidence is insufficient to suggest whether routine human immunodeficiency virus(HIV)screening in healthcare settings is effective in promoting greater awareness...Summary What is already known about this topic?Research evidence is insufficient to suggest whether routine human immunodeficiency virus(HIV)screening in healthcare settings is effective in promoting greater awareness of HIV-positive status.What is added by this report?This study found that,following the implementation of routine HIV screening in hospitals in Xishuangbanna Prefecture,Yunnan Province,there was a significant increase in the number of HIV screenings,positive results,and the positive rate of HIV screening in primary-level hospitals.What are the implications for public health practice?Routine hospital-based HIV screening is effective in identifying HIV infections in areas with concentrated epidemics.展开更多
基金supported by grants from the National Natural Science Foundation of China for Dai (Grant No. 81472188) and Zheng (Grant No. 81702247)the Youth Development Project of TMMU for Zheng (Grant No. 2016XPY17)
文摘Objective: Cancer vaccines that rely on tumor antigen-specific CD8^+ T cell responses, are promising anti-cancer adjuvant immunotherapies. This study investigated whether genetically attenuated Plasmodium sporozoite(GAS) could be used as a novel vector to induce antigen-specific CD8^+ T cell responses against lung cancer.Methods: We constructed GAS/MAGE-A3, a recombinant GAS engineered to express the lung cancer-specific antigen, melanomaassociated antigen 3(MAGE-A3), and assessed its therapeutic effects against lung cancer.Results: Robust parasite-specific CD8αlow CD11ahigh and CD49dhigh CD11ahigh CD4^+ T cell responses as well as a MAGE-A3-specific CD8^+ T cell response were induced in GAS/MAGE-A3-immunized mice. Adoptive transfer of GAS/MAGE-A3-induced CD8^+ T cells from HLA-A2 transgenic mice into lung cancer-bearing nude mice inhibited tumor growth and prolonged survival.Conclusions: These findings demonstrate that GAS/MAGE-A3 induces a strong MAGE-A3-specific CD8^+ T cell response against lung cancer in vivo, and indicate that GAS is a novel and efficacious antigen delivery vector for antitumor immunotherapy.
基金Supported by the China National Science and Technology Major Projects in Infectious Disease from the Ministry of Science and Technology[Grant no.2017ZX10201101-002-003].
文摘Summary What is already known about this topic?Research evidence is insufficient to suggest whether routine human immunodeficiency virus(HIV)screening in healthcare settings is effective in promoting greater awareness of HIV-positive status.What is added by this report?This study found that,following the implementation of routine HIV screening in hospitals in Xishuangbanna Prefecture,Yunnan Province,there was a significant increase in the number of HIV screenings,positive results,and the positive rate of HIV screening in primary-level hospitals.What are the implications for public health practice?Routine hospital-based HIV screening is effective in identifying HIV infections in areas with concentrated epidemics.
