Objective： To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens（MSP） flower extracton on in vitro and in vivo models.Methods： MSP flower extract was extracted in 99.9% methanol a...Objective： To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens（MSP） flower extracton on in vitro and in vivo models.Methods： MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells（h DPCs） in vitro at the dose of 3.92-62.50 μg/m L and hair growth of C57 BL/6 mice in vivo at the dose of 1000 μg/m L.The expression of transforming growth factor β1（TGF-β1）,hepatocyte growth factor（HGF）,β-catenin,substance P was measured by relative quantitative realtime polymerase chain reaction.Histopathological and immunohistochemical analysis were performed.Results： MSP（7.81 μg/m L） down-regulated TGF-β1 and up-regulated HGF and β-catenin in h DPCs（P〈0.01）.MSP（1000 μg/m L）-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase.The number of mast cells was lower in the MSP-treated mice than in other groups（P〈0.05 vs.NCS group）.Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control.Stem cell factor in hair follicles was up-regulated in the MSP-treated mice（P〈0.01）.Conclusion： The MSP flower extract may have hair growth-promotion activities.展开更多
基金Supported by Business for Cooperative R&D between Industry,Academy,and Research Institute funded Korea Small and Medium Business Administration(No.C0142798)in 2013the Chung-Ang University Research Grants in 2015
文摘Objective： To investigate the hair growth-promoting effect of Miscanthus sinensis var.purpurascens（MSP） flower extracton on in vitro and in vivo models.Methods： MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells（h DPCs） in vitro at the dose of 3.92-62.50 μg/m L and hair growth of C57 BL/6 mice in vivo at the dose of 1000 μg/m L.The expression of transforming growth factor β1（TGF-β1）,hepatocyte growth factor（HGF）,β-catenin,substance P was measured by relative quantitative realtime polymerase chain reaction.Histopathological and immunohistochemical analysis were performed.Results： MSP（7.81 μg/m L） down-regulated TGF-β1 and up-regulated HGF and β-catenin in h DPCs（P〈0.01）.MSP（1000 μg/m L）-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase.The number of mast cells was lower in the MSP-treated mice than in other groups（P〈0.05 vs.NCS group）.Substance P and TGF-β1 were expressed in hair follicles and skin of the MSP group lower than that in negative control.Stem cell factor in hair follicles was up-regulated in the MSP-treated mice（P〈0.01）.Conclusion： The MSP flower extract may have hair growth-promotion activities.
