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Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hvbridization
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作者 QIU Jiwen JIN Demin +5 位作者 LI Chuanyou JIA Jianhang OUYANG Ping william tai WANG Bin LI Dawei 《Chinese Science Bulletin》 SCIE EI CAS 2000年第6期528-532,共5页
RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 3... RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed. 展开更多
关键词 bread wheat Tripsacum dactyloides wide hybridization RAPD RFLP.
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