Fragaria vesca,a wild diploid strawberry,serves as a fundamental research model for cultivated strawberry.The current reference genomes available are limited to two closely-related accessions,Hawaii 4 and CFRA2339.The...Fragaria vesca,a wild diploid strawberry,serves as a fundamental research model for cultivated strawberry.The current reference genomes available are limited to two closely-related accessions,Hawaii 4 and CFRA2339.The widely-used model accession‘Yellow Wonder'does not yet have its reference genome.In this study,the genome of a 7^(th) generation inbred'Yellow Wonder'was assembled using a combination of Oxford Nanopore long reads and Illumina short reads.The de novo chromosome-scale assembly of this 220 megabase genome possesses 34,007 genes which were annotated through lift over from the Hawaii 4 genome annotation.Genome comparisons show that the‘Yellow Wonder'genome is relatively distinct from the two previously published F.vesca accessions,Hawaii 4 and CFRA2339.The availability of a‘Yellow Wonder'reference genome adds another important genomic resource to Fragaria vesca and enables rapid research progress in strawberry.展开更多
基金This research was supported by a University of Maryland Faculty-Student Research Grant to DJ and ZL,University of Maryland CMNS Dean's Matching Award to DJ that is associated with the UMD NIH T32 Molecular and Cell Biology Training Grant,a University of Maryland Hockmeyer Fellowship to DJ,and National Science Foundation grant(IOS1444987)to ZL The computational resources were provided by Cyverse(www.cyverse.org)which is supported by the National Science Foundation grants DBI-0735191,DBI-1265383,and DBI-1743442.
文摘Fragaria vesca,a wild diploid strawberry,serves as a fundamental research model for cultivated strawberry.The current reference genomes available are limited to two closely-related accessions,Hawaii 4 and CFRA2339.The widely-used model accession‘Yellow Wonder'does not yet have its reference genome.In this study,the genome of a 7^(th) generation inbred'Yellow Wonder'was assembled using a combination of Oxford Nanopore long reads and Illumina short reads.The de novo chromosome-scale assembly of this 220 megabase genome possesses 34,007 genes which were annotated through lift over from the Hawaii 4 genome annotation.Genome comparisons show that the‘Yellow Wonder'genome is relatively distinct from the two previously published F.vesca accessions,Hawaii 4 and CFRA2339.The availability of a‘Yellow Wonder'reference genome adds another important genomic resource to Fragaria vesca and enables rapid research progress in strawberry.
