Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

瞄准：检验是否 trans-10， cis-12 CLA (t10c12 ) 或 cis-9， trans-11 CLA (c9t11 ) 禁止 heregulin (HRG )-beta-stimulated 细胞生长并且 HRG-beta-ErbB3 在 HT-29 细胞发信号。方法：我们在 CLA 异构体或 ErbB3 ligand HRG 贝它的缺席或存在的有教养的 HT-29 房间。MTT 试金，[3H ] 胸腺嘧啶核甙加入，染色的 Annexin V， RT-PCR，西方的弄污，免疫降水，和在试管内激酶试金被执行。结果：HRG 贝它增加了房间生长，但是没阻止导致 t10c12 的生长抑制。T10c12 禁止了 DNA 合成并且导致了 HT-29 房间的 apoptosis，而 c9t11 没有效果。T10c12 减少了以一种剂量依赖者方式的 ErbB1， ErbB2，和 ErbB3 蛋白质和抄本的层次，而 c9t11 没有效果。Immunoprecipitation/Western 污点研究表明 t10c12 禁止了 ErbB3，到 ErbB3 的 phosphoinositide 3-kinase (PI3K ) 的 p85 子单元的招募，联系 ErbB3 的 PI3K 活动，和 Akt 的磷酸化的 HRG-beta-stimulated 磷酸化。然而， c9t11 没在 phospho Akt 层次上有效果。既不 t10c12 也不 c9t11 在 ERK-1/2 的 HRG-beta-induced 磷酸化上有任何效果。结论：这些结果显示由 t10c12 的 HT-29 细胞生长的抑制可以经由它发信号导致 Akt 激活的抑制的 ErbB3 的调整被导致。

Effect of rGO support on Gd@ZnO for UV-visible-light driven photocatalytic organic pollutant degradation

In this manuscript,we report the hydrothermally prepared rGO/Gd@ZnO nanocomposite for the utilization of photocatalytic Rh B(rhodamine B)dye degradation.The required characterization techniques were carried out to analyse the structural,morphological,chemical,environmental and optical behaviour of the prepared nanoparticles.Photocatalytic efficiency of the prepared nanoparticles was studied by Rh B dye degradation upon UV-Vis-light illumination which reveals that composite catalyst degrades the dye molecule about 91%after 40 min of irradiation.On comparing the higher degradation efficiency of the rGO/Gd@ZnO nanocomposite with that of ZnO and Gd doped ZnO,it reveals a greater reduction of charge carriers'recombination,higher visible-light utilization and higher adsorption efficiency.Elemental trapping experiment and recycling test were conducted to study the influence of active radicals for the degradation reaction and to know the stability of the prepared nanocomposite,respectively.Hence,this rGO supported Gd doped ZnO nanocomposite can provide a new path way in preparing a highly efficient photocatalyst for organic pollutant degradation at large.