Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objec...Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.展开更多
Allergic contact dermatitis (ACD) affects approximately 7%of the general population. To evaluate the frequency of ACD in children,we analysed patch test results collected by the Information Network of Departments of D...Allergic contact dermatitis (ACD) affects approximately 7%of the general population. To evaluate the frequency of ACD in children,we analysed patch test results collected by the Information Network of Departments of Dermatology between 1995 and 2002. Data of 285 children (6-12 year) and 2175 adolescent patients (13-18 year) were analysed to determine the frequency of sensitization to the 30 most common contact allergens,adjusting for age and sex. As control group,we defined adult patients (60-66 year,n=7904). The top allergens in children were thimerosal,gentamicin sulphate,nickel-II-sulphate,ammoniated mercury,cobalt-II-chloride,fragrance mix,bufexamac,Compositae mix,propylene glycol and turpentine. The overall proportion of sensitized patients according to the patch test results was 52.6%in the children group compared to 49.7%in the adolescent group. These findings were similar in the adult group at 52.2%. The detailed analysis regarding sex,occupation,atopy,site of eczema and age showed distinct patterns in each group indicating age-specific exposures. Atopy-related diseases were more common in children compared to adults. On the basis of the data of this study,the relationship between atopy and the risk of development of ACD,at least in children,needs further investigation.展开更多
文摘Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.
文摘Allergic contact dermatitis (ACD) affects approximately 7%of the general population. To evaluate the frequency of ACD in children,we analysed patch test results collected by the Information Network of Departments of Dermatology between 1995 and 2002. Data of 285 children (6-12 year) and 2175 adolescent patients (13-18 year) were analysed to determine the frequency of sensitization to the 30 most common contact allergens,adjusting for age and sex. As control group,we defined adult patients (60-66 year,n=7904). The top allergens in children were thimerosal,gentamicin sulphate,nickel-II-sulphate,ammoniated mercury,cobalt-II-chloride,fragrance mix,bufexamac,Compositae mix,propylene glycol and turpentine. The overall proportion of sensitized patients according to the patch test results was 52.6%in the children group compared to 49.7%in the adolescent group. These findings were similar in the adult group at 52.2%. The detailed analysis regarding sex,occupation,atopy,site of eczema and age showed distinct patterns in each group indicating age-specific exposures. Atopy-related diseases were more common in children compared to adults. On the basis of the data of this study,the relationship between atopy and the risk of development of ACD,at least in children,needs further investigation.
