目的探讨回盲部癌伴周围脓肿(ileocecal carcinoma with peripheral abscess,ICPA)的临床特点及诊治方法。方法报告川北医学院附属医院收治的1例ICPA病历资料,并检索到国内外文献报道的64例ICPA。对65例ICPA的临床特征、诊断及治疗进行...目的探讨回盲部癌伴周围脓肿(ileocecal carcinoma with peripheral abscess,ICPA)的临床特点及诊治方法。方法报告川北医学院附属医院收治的1例ICPA病历资料,并检索到国内外文献报道的64例ICPA。对65例ICPA的临床特征、诊断及治疗进行分析。结果此例病人行右半结肠切除术+髂窝脓肿病灶清除术+氟尿嘧啶灌洗术+大网膜填塞髂窝脓腔术,术后伤口Ⅰ期甲级愈合,连续6个周期FOLFOX化疗,随访1年,肿瘤未复发。ICPA病人有右下腹疼痛、发热、白细胞升高、右下腹包块等类似阑尾周围脓肿的症状及体征;93.85%的病人被漏诊或误诊,术前初步诊断为阑尾周围脓肿者占87.69%;病理诊断中,腺癌占75.38%,黏液腺癌为23.08%;73.84%的病人行右半结肠切除根治术,21.54%的病人无法切除而行姑息治疗。结论ICPA容易误诊为阑尾周围脓肿,导致耽误病情及治疗时机,右半结肠切除术可达到更好的根治标准,处理特殊的回盲部癌周围的深部脓肿,大网膜填塞是一种可选的较好方法,氟尿嘧啶灌洗也可改善病人的预后。展开更多
目的探讨子宫动脉栓塞术(UAE)联合B超引导下清宫术治疗剖宫产瘢痕妊娠(CSP)患者的临床效果。方法选择2012年1月至2020年12月镇江市第四人民医院收治的100例CSP患者为研究对象,按照手术方式的不同将其分为对照组与试验组,每组50例。对照...目的探讨子宫动脉栓塞术(UAE)联合B超引导下清宫术治疗剖宫产瘢痕妊娠(CSP)患者的临床效果。方法选择2012年1月至2020年12月镇江市第四人民医院收治的100例CSP患者为研究对象,按照手术方式的不同将其分为对照组与试验组,每组50例。对照组采用B超引导下清宫术治疗,试验组采用UAE联合B超引导下清宫术治疗,比较两组手术前后人绒毛膜促性腺激素(HCG)水平、术后子宫内膜厚度、手术相关指标、术后疼痛程度[采用视觉模拟评分法(VAS)评估]、术后并发症发生情况以及手术结局。结果术前,两组HCG水平比较,差异无统计学意义(P>0.05);术后第1天,试验组HCG水平高于对照组,差异有统计学意义(P<0.05);术后第2天,试验组HCG水平低于对照组,子宫内膜厚度大于对照组,差异有统计学意义(P<0.05);试验组术中出血量少于对照组,HCG降低至正常水平用时及住院时间均短于对照组,术后12 h VAS评分低于对照组,差异有统计学意义(P<0.05);两组术后并发症发生率比较,差异无统计学意义(P>0.05);试验组术后妊娠组织残留、大出血、子宫切除及转行宫腔镜手术患者占比均低于对照组,差异有统计学意义(P<0.05)。结论UAE联合B超引导下清宫术治疗剖宫产瘢痕妊娠(CSP)患者的效果显著,具有创伤小、术后并发症发生率低、疼痛程度轻等优势,可最大限度保留患者子宫,促进患者康复。展开更多
The reaction between Laccase and o methoxyphenol have been studied by LKB 2107 batch microcalorimetry system. Thermodynamic parameters Δ rH m , Δ G 0 , Δ G ≠ T ,) and kinetic parameters ( K m,k...The reaction between Laccase and o methoxyphenol have been studied by LKB 2107 batch microcalorimetry system. Thermodynamic parameters Δ rH m , Δ G 0 , Δ G ≠ T ,) and kinetic parameters ( K m,k 2 ) have been determined. The process of the reaction has been analyzed from changes in energy by using the transition state theory. Two methods for enhancing catalytic power of Laccase are proposed. The results shown that formation of an enzyme substrate complex is“anticatalytic”. The enter and sole source of catalytic power is the stabilization of transition state; reactant state interactions are by nature inhibitory and only waste catalytic power.展开更多
The molar reation enthalpy,the Michaelis constant and the observed rate constant of the reaction between the Rhus vernicifera laccase and p-phenylenediamine have been determined at 298. 15 K by LKB-2107 microcalorimet...The molar reation enthalpy,the Michaelis constant and the observed rate constant of the reaction between the Rhus vernicifera laccase and p-phenylenediamine have been determined at 298. 15 K by LKB-2107 microcalorimetry system in 0.1 mol/L phosphate salt buffer (pH7. 4) to be △rHm=-136.36±0. 36kJ/mol, Km= 5. 58×10-3 mol/L and k1 =8. 63×10-3s-1, respectively. The catalyst activity of laccase withp-phenylenediamine as substrate has been determined to be EA=0. 045 IU in the experimental condition.The observed activation energy of non-enzymic step of the reaction, the Gibbs binding energy of the combination process of laccase and substrate have been also calculated. The physical significance of the determined parameters were discussed for different step of the reaction.展开更多
The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distingushing inhibition type was given and the methods for calculat...The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distingushing inhibition type was given and the methods for calculating kinetic parameters, KM,Ki and Urn were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation of o-dihydroxybenzene by m-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type, KM=6.224×10-3 mol L-1, Ki=2. 363 × 10-2 mol. L-1.展开更多
文摘目的探讨回盲部癌伴周围脓肿(ileocecal carcinoma with peripheral abscess,ICPA)的临床特点及诊治方法。方法报告川北医学院附属医院收治的1例ICPA病历资料,并检索到国内外文献报道的64例ICPA。对65例ICPA的临床特征、诊断及治疗进行分析。结果此例病人行右半结肠切除术+髂窝脓肿病灶清除术+氟尿嘧啶灌洗术+大网膜填塞髂窝脓腔术,术后伤口Ⅰ期甲级愈合,连续6个周期FOLFOX化疗,随访1年,肿瘤未复发。ICPA病人有右下腹疼痛、发热、白细胞升高、右下腹包块等类似阑尾周围脓肿的症状及体征;93.85%的病人被漏诊或误诊,术前初步诊断为阑尾周围脓肿者占87.69%;病理诊断中,腺癌占75.38%,黏液腺癌为23.08%;73.84%的病人行右半结肠切除根治术,21.54%的病人无法切除而行姑息治疗。结论ICPA容易误诊为阑尾周围脓肿,导致耽误病情及治疗时机,右半结肠切除术可达到更好的根治标准,处理特殊的回盲部癌周围的深部脓肿,大网膜填塞是一种可选的较好方法,氟尿嘧啶灌洗也可改善病人的预后。
文摘目的探讨子宫动脉栓塞术(UAE)联合B超引导下清宫术治疗剖宫产瘢痕妊娠(CSP)患者的临床效果。方法选择2012年1月至2020年12月镇江市第四人民医院收治的100例CSP患者为研究对象,按照手术方式的不同将其分为对照组与试验组,每组50例。对照组采用B超引导下清宫术治疗,试验组采用UAE联合B超引导下清宫术治疗,比较两组手术前后人绒毛膜促性腺激素(HCG)水平、术后子宫内膜厚度、手术相关指标、术后疼痛程度[采用视觉模拟评分法(VAS)评估]、术后并发症发生情况以及手术结局。结果术前,两组HCG水平比较,差异无统计学意义(P>0.05);术后第1天,试验组HCG水平高于对照组,差异有统计学意义(P<0.05);术后第2天,试验组HCG水平低于对照组,子宫内膜厚度大于对照组,差异有统计学意义(P<0.05);试验组术中出血量少于对照组,HCG降低至正常水平用时及住院时间均短于对照组,术后12 h VAS评分低于对照组,差异有统计学意义(P<0.05);两组术后并发症发生率比较,差异无统计学意义(P>0.05);试验组术后妊娠组织残留、大出血、子宫切除及转行宫腔镜手术患者占比均低于对照组,差异有统计学意义(P<0.05)。结论UAE联合B超引导下清宫术治疗剖宫产瘢痕妊娠(CSP)患者的效果显著,具有创伤小、术后并发症发生率低、疼痛程度轻等优势,可最大限度保留患者子宫,促进患者康复。
文摘The reaction between Laccase and o methoxyphenol have been studied by LKB 2107 batch microcalorimetry system. Thermodynamic parameters Δ rH m , Δ G 0 , Δ G ≠ T ,) and kinetic parameters ( K m,k 2 ) have been determined. The process of the reaction has been analyzed from changes in energy by using the transition state theory. Two methods for enhancing catalytic power of Laccase are proposed. The results shown that formation of an enzyme substrate complex is“anticatalytic”. The enter and sole source of catalytic power is the stabilization of transition state; reactant state interactions are by nature inhibitory and only waste catalytic power.
文摘The molar reation enthalpy,the Michaelis constant and the observed rate constant of the reaction between the Rhus vernicifera laccase and p-phenylenediamine have been determined at 298. 15 K by LKB-2107 microcalorimetry system in 0.1 mol/L phosphate salt buffer (pH7. 4) to be △rHm=-136.36±0. 36kJ/mol, Km= 5. 58×10-3 mol/L and k1 =8. 63×10-3s-1, respectively. The catalyst activity of laccase withp-phenylenediamine as substrate has been determined to be EA=0. 045 IU in the experimental condition.The observed activation energy of non-enzymic step of the reaction, the Gibbs binding energy of the combination process of laccase and substrate have been also calculated. The physical significance of the determined parameters were discussed for different step of the reaction.
文摘The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced, and then the criteria for distingushing inhibition type was given and the methods for calculating kinetic parameters, KM,Ki and Urn were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation of o-dihydroxybenzene by m-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type, KM=6.224×10-3 mol L-1, Ki=2. 363 × 10-2 mol. L-1.
