桑白皮水煎总提物及拆分组分对肾阴虚水肿模型大鼠的影响

目的:研究桑白皮水煎总提物及拆分组分对肾阴虚水肿模型的影响,为桑白皮的药性研究提供参考。方法:采用尾静脉注射阿霉素结合甲状腺片灌胃法建立大鼠肾阴虚水肿模型,造模结束后灌胃给予桑白皮水煎总提物及各拆分组分4周,检测血清环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、睾酮(T)、雌二醇(E_2)、三碘甲状腺原氨酸(T_3)、甲状腺素(T_4)、肌酐(Cr)、尿素氮(BUN)、甘油三酯(TG)、总胆固醇(TC)及尿蛋白等指标,考察桑白皮水煎总提物及各拆分组分对肾阴虚水肿模型大鼠的影响。结果:桑白皮水煎总提物、桑白皮多糖拆分组分和脂肪油拆分组分能显著降低血清cAMP、E_2、T_3、T_4、Cr、BUN、TG、TC及尿蛋白水平(P<0.05或P<0.01),升高血清cGMP、T的水平(P<0.05或P<0.01)。结论:中医临床认为,气为阳,血为阴,阴虚生内热,阳虚生外寒,而肾阴虚水肿病症本在肾,标在肺。桑白皮水煎总提物及其拆分组分能显著改善肾阴虚水肿模型大鼠的病理状况,提示这可能与桑白皮"味甘,性寒,归肺经""泻肺平喘,利水消肿""补虚"的性能功效有关。

麻黄水煎液及拆分组分对大鼠利尿作用的实验研究

目的：研究麻黄水煎液及拆分组分的利尿作用,并初步探讨其作用机制。方法：将水负荷大鼠分为空白对照组,氢氯噻嗪组（0.017 g·kg-1）,麻黄水煎液组（2.34 g·kg-1）,生物碱组（0.020 g·kg-1）,非生物碱组（0.105 g·kg-1）,醇沉组（0.132 g·kg-1）,挥发油组（0.9333×10-3m L·kg-1）,各组给予相应药物,空白对照组给予同体积生理盐水。采用大鼠代谢笼法,观察麻黄水煎液及拆分组分对大鼠尿量的影响;微板法检测尿氯（Cl-）、尿钾（K＋）水平;比浊法检测尿钠（Na＋）水平;离子选择电极法检测尿渗透压;酶联免疫吸附测定法（ELISA）检测血浆抗利尿激素（ADH）,肾脏水通道蛋白2（AQP2）水平;蛋白免疫印迹法（Western Blot）检测肾脏水通道蛋白2（AQP2）水平。结果：与空白对照组相比,麻黄水煎液和生物碱组分能显著增加大鼠尿量和尿液电解质排泄,降低尿渗透压,ADH,AQP2水平（P〈0.01或P〈0.05）。结论：麻黄具有显著的利尿作用,其利尿作用的组分主要为生物碱,且发挥利尿作用的机制可能与降低ADH的水平和AQP2的表达有关。

Different meridian tropism in three Chinese medicines: Tinglizi(Semen Lepidii Apetali), Yiyiren(Semen Coicis), Cheqianzi(Semen Plantaginis)

OBJECTIVE: To study the meridian tropism(MT) for three Chinese medicines: Tinglizi(Semen Lepidii Apetali, TLZ), Yiyiren(Semen Coicis, YYR), Cheqianzi(Semen Plantaginis, CQZ) in vivo experiments.METHODS: The extracts of TLZ,YYR, CQZ were orally administered to rats at a dose 234, 700 and 350 mg/kg, respectively. The urinary excretion rate, loss in weight of rats and the electrolyte levels in the plasma were measured, in order to assess the mechanisms of the three traditional Chinese diuretic medicines.RESULTS: TLZ, YYR and CQZ exhibited obvious diuretic effect and reduced the weight of saline-loaded animals. All the three diuretic medicines differentially reduced various serum, urinary, lung, stomach, kidney and hormonal markers, thus indicating the different mechanisms of action and a close resemblance with their different meridian as per the MT theory.CONCLUSION: Our findings suggest that the three Traditional Chinese Medicine(TCM) medicines-TLZ,YYR and CQZ-possess different Shamisen meridian.This can support the regimen of treating the same disease with different therapies in TCM theory.