目的探讨机器人援助腹腔镜操作在复杂性子宫切除术中的可行性及优势。方法回顾性分析经机器人腹腔镜下复杂性子宫切除术的36例患者以及同期进行传统腹腔镜下复杂性子宫切除术的85例患者的临床资料,包括患者手术时长、术中失血量、首次...目的探讨机器人援助腹腔镜操作在复杂性子宫切除术中的可行性及优势。方法回顾性分析经机器人腹腔镜下复杂性子宫切除术的36例患者以及同期进行传统腹腔镜下复杂性子宫切除术的85例患者的临床资料,包括患者手术时长、术中失血量、首次肛门排气时间、术后住院天数、术后12 h疼痛视觉模拟量表(VAS)评分、是否中转开腹、手术并发症(肠梗阻、切口疝、切口感染、双下肢深静脉血栓、泌尿系损伤、阴道断端愈合不良)。结果机器人组患者的子宫体积、术中失血量、首次肛门排气时间、术后住院天数、术后12 h VAS评分、中转开腹率均低于腹腔镜组,手术时间略长于腹腔镜组,差异有统计学意义(P<0.05)。2组患者术后并发症(肠梗阻、切口疝、切口感染、双下肢深静脉血栓、泌尿系损伤、阴道断端愈合不良)发生率比较,差异无统计学意义(P>0.05)。结论机器人腹腔镜手术定位精准,操作灵活,能明显减轻术后不适,减少术中出血量,促进患者恢复,适合复杂性子宫切除术。展开更多
Objective:To identify a full length cDNA sequence of a novel tetraspanin(TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.M...Objective:To identify a full length cDNA sequence of a novel tetraspanin(TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:Using the NCBI,EMBI,Expasy and other online sites, the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,transmembrane domain,epitope,topological structures of the protein sequences were predicted.And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.Results:’Hie target sequence was 1 132 hp length with a 681 hp biggest ORF encoding 226 amino acids protein with typical TSP conserved domain.It was confirmed as full length cDNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number:JF728872).The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da,respectively.Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni.it showed similarity of 59%and 59%, respectively.SeTSP16 contained four transmembrane domains(TM 1-4),intracellular N and C-termini,one short small extracellular loop and one large extracellular loop.Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted.Conclusions:The full length cDNA sequences of SeTSP16 arc identified.It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs.展开更多
文摘目的探讨机器人援助腹腔镜操作在复杂性子宫切除术中的可行性及优势。方法回顾性分析经机器人腹腔镜下复杂性子宫切除术的36例患者以及同期进行传统腹腔镜下复杂性子宫切除术的85例患者的临床资料,包括患者手术时长、术中失血量、首次肛门排气时间、术后住院天数、术后12 h疼痛视觉模拟量表(VAS)评分、是否中转开腹、手术并发症(肠梗阻、切口疝、切口感染、双下肢深静脉血栓、泌尿系损伤、阴道断端愈合不良)。结果机器人组患者的子宫体积、术中失血量、首次肛门排气时间、术后住院天数、术后12 h VAS评分、中转开腹率均低于腹腔镜组,手术时间略长于腹腔镜组,差异有统计学意义(P<0.05)。2组患者术后并发症(肠梗阻、切口疝、切口感染、双下肢深静脉血栓、泌尿系损伤、阴道断端愈合不良)发生率比较,差异无统计学意义(P>0.05)。结论机器人腹腔镜手术定位精准,操作灵活,能明显减轻术后不适,减少术中出血量,促进患者恢复,适合复杂性子宫切除术。
文摘Objective:To identify a full length cDNA sequence of a novel tetraspanin(TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:Using the NCBI,EMBI,Expasy and other online sites, the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,transmembrane domain,epitope,topological structures of the protein sequences were predicted.And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.Results:’Hie target sequence was 1 132 hp length with a 681 hp biggest ORF encoding 226 amino acids protein with typical TSP conserved domain.It was confirmed as full length cDNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number:JF728872).The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da,respectively.Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni.it showed similarity of 59%and 59%, respectively.SeTSP16 contained four transmembrane domains(TM 1-4),intracellular N and C-termini,one short small extracellular loop and one large extracellular loop.Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted.Conclusions:The full length cDNA sequences of SeTSP16 arc identified.It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs.
