OBJECTIVE: To evaluate the efficacy of herb-partitioned moxibustion(HPM) at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints in relieving symptoms and the immune regulation of HPM on the toll-like receptors 4(T...OBJECTIVE: To evaluate the efficacy of herb-partitioned moxibustion(HPM) at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints in relieving symptoms and the immune regulation of HPM on the toll-like receptors 4(TLR4) signaling pathway in ulcerative colitis(UC) patients.METHODS: A randomized, single-blind study was conducted 63 patients to receive HPM or sham HPM treatment. The efficacy outcomes included scores of the Mayo, Baron, inflammatory bowel disease questionnaire(IBDQ), self-rating depression scale(SDS), self-rating anxiety scale(SAS). HE staining was used to observe the histopathological changes of the colon. The expression of inflammatory cytokines and TLR4 signaling pathway related molecules were determined by enzyme-linked immunosorbent assay and immunohistochemistry.RESULTS: Baron, SDS, SAS scores were significantly decreased in moxibustion group(P < 0.05), IBDQ score was significantly greater in the moxibustion group than in the sham moxibustion group(P < 0.05). Histopathology of mucosal biopsies showed that both two groups improved in mucosa after treatment. The expression levels of tumor necrosis factor-α, interleukin-2, interleukin-12, interferon-γ, and TLR4, lipopolysaccharide, myeloid differentiation factor 88, interleukin receptor associated kinase, tumor necrosis factor receptor associated factor 6 and nuclear factor kappa-B p65 were significantly lower in the moxibustion group than in the sham moxibustion group(P < 0.05).CONCLUSION: This study showed that HPM at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints is effective to relieve symptoms, anxiety, depression and improving life quality in UC patients,which may be related to the immune regulation of HPM on TLR4 signaling pathway.展开更多
OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine r...OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.展开更多
Objective:To observe the effect of moxibustion on the colonic mucosal barrier of rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods:Forty male Sprague-Dawley rats were randomly divided into...Objective:To observe the effect of moxibustion on the colonic mucosal barrier of rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods:Forty male Sprague-Dawley rats were randomly divided into a normal group and a modeling group,with 20 rats in each group.Rats in the modeling group were subjected to preparing experimental UC models by drinking 4%DSS for seven consecutive days.Two modeled rats and two normal rats were randomly selected for model identification.After the success of UC model was confirmed,the remaining 18 modeled rats were randomly divided into three groups,a model group,a model+herbal cake-partitioned moxibustion group,and a model+mild moxibustion group,with six rats in each group;the remaining normal rats were randomly divided into three groups,a normal group,a normal+herbal cake-partitioned moxibustion group,and a normal+mild moxibustion group,with six rats in each group.After 7 d of intervention with the herbal cake-partitioned moxibustion or the mild moxibustion,hematoxylin-eosin(HE)staining technique was used to observe the pathological changes of colon tissue under a light microscope;Western blotting and/or immunohistochemical techniques were used to detect the protein expression levels of Occludin,Claudin,junction adhesion molecular 1(JAM1),mucin 2(MUC2),and transforming growth factor beta1(TGF-β1)in rat colon tissue.Results:Compared with the normal group,the colon tissue was severely damaged,the pathological score was significantly increased,and the protein expression levels of Occludin,Claudin,JAM1,MUC2,and TGF-β1 were significantly decreased in the model group(P<0.01);while there were no significant differences in the colonic histopathological score,protein expression levels of Occludin,Claudin,JAM1,MUC2,and TGF-β1 in the normal+herbal cake-partitioned moxibustion group and the normal+mild moxibustion group(P>0.05).Compared with the model group,the model+herbal cake-partitioned moxibustion group and the model+mild moxibustion group showed repaired colon tissue,ulcer healing,significantly reduced pathological score,and significantly increased protein expression levels of JAM1,MUC2,and TGF-β1(P<0.05);the Occludin protein expression level in the colon tissue of the model+mild moxibustion group was increased(P<0.01).Conclusion:Neither herbal cake-partitioned moxibustion nor mild moxibustion influences the colonic histopathology and intestinal mucosal barrier-related protein expression in the normal rats;both herbal cake-partitioned moxibustion and mild moxibustion can up-regulate the protein expression levels of JAM1,MUC2,and TGF-β1 in the colon tissue of UC rats.Mild moxibustion can up-regulate Occludin protein expression.This may be a mechanism of moxibustion in reducing colonic mucosa inflammation in UC.展开更多
基金Supported by grants from the National Natural Science Foundation of China (No.81674074, 81774405, 81704161)three-year Action Plan Project of Shanghai Traditional Chinese Medicine Development [No.ZY(2018-2020)-CCCX-2004-01]+2 种基金project of Shanghai Municipal Health Commission (No.201840096)shanghai Municipal population and Family planning Commission "Excellent Youth Talent Training Program"(No.2018YQ11)Special Clinical Research Project in the Health Industry of Shanghai Municipal Health Commission (No.20204Y0245)。
文摘OBJECTIVE: To evaluate the efficacy of herb-partitioned moxibustion(HPM) at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints in relieving symptoms and the immune regulation of HPM on the toll-like receptors 4(TLR4) signaling pathway in ulcerative colitis(UC) patients.METHODS: A randomized, single-blind study was conducted 63 patients to receive HPM or sham HPM treatment. The efficacy outcomes included scores of the Mayo, Baron, inflammatory bowel disease questionnaire(IBDQ), self-rating depression scale(SDS), self-rating anxiety scale(SAS). HE staining was used to observe the histopathological changes of the colon. The expression of inflammatory cytokines and TLR4 signaling pathway related molecules were determined by enzyme-linked immunosorbent assay and immunohistochemistry.RESULTS: Baron, SDS, SAS scores were significantly decreased in moxibustion group(P < 0.05), IBDQ score was significantly greater in the moxibustion group than in the sham moxibustion group(P < 0.05). Histopathology of mucosal biopsies showed that both two groups improved in mucosa after treatment. The expression levels of tumor necrosis factor-α, interleukin-2, interleukin-12, interferon-γ, and TLR4, lipopolysaccharide, myeloid differentiation factor 88, interleukin receptor associated kinase, tumor necrosis factor receptor associated factor 6 and nuclear factor kappa-B p65 were significantly lower in the moxibustion group than in the sham moxibustion group(P < 0.05).CONCLUSION: This study showed that HPM at Qihai(CV6), Tianshu(ST25) and Shangjuxu(ST37) acupoints is effective to relieve symptoms, anxiety, depression and improving life quality in UC patients,which may be related to the immune regulation of HPM on TLR4 signaling pathway.
基金Supported by the National Basic Research Program of China(973 Program,No.2015CB554501)Program of National Natural Science Foundation of China(No.81574079)+2 种基金Program for outstanding medical academic leader(2015,No.80)Shuguang Program of Shanghai Education Commission(No.14SG39)Shanghai Rising-Star Program(No.16QA1403400)
文摘OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.
文摘Objective:To observe the effect of moxibustion on the colonic mucosal barrier of rats with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods:Forty male Sprague-Dawley rats were randomly divided into a normal group and a modeling group,with 20 rats in each group.Rats in the modeling group were subjected to preparing experimental UC models by drinking 4%DSS for seven consecutive days.Two modeled rats and two normal rats were randomly selected for model identification.After the success of UC model was confirmed,the remaining 18 modeled rats were randomly divided into three groups,a model group,a model+herbal cake-partitioned moxibustion group,and a model+mild moxibustion group,with six rats in each group;the remaining normal rats were randomly divided into three groups,a normal group,a normal+herbal cake-partitioned moxibustion group,and a normal+mild moxibustion group,with six rats in each group.After 7 d of intervention with the herbal cake-partitioned moxibustion or the mild moxibustion,hematoxylin-eosin(HE)staining technique was used to observe the pathological changes of colon tissue under a light microscope;Western blotting and/or immunohistochemical techniques were used to detect the protein expression levels of Occludin,Claudin,junction adhesion molecular 1(JAM1),mucin 2(MUC2),and transforming growth factor beta1(TGF-β1)in rat colon tissue.Results:Compared with the normal group,the colon tissue was severely damaged,the pathological score was significantly increased,and the protein expression levels of Occludin,Claudin,JAM1,MUC2,and TGF-β1 were significantly decreased in the model group(P<0.01);while there were no significant differences in the colonic histopathological score,protein expression levels of Occludin,Claudin,JAM1,MUC2,and TGF-β1 in the normal+herbal cake-partitioned moxibustion group and the normal+mild moxibustion group(P>0.05).Compared with the model group,the model+herbal cake-partitioned moxibustion group and the model+mild moxibustion group showed repaired colon tissue,ulcer healing,significantly reduced pathological score,and significantly increased protein expression levels of JAM1,MUC2,and TGF-β1(P<0.05);the Occludin protein expression level in the colon tissue of the model+mild moxibustion group was increased(P<0.01).Conclusion:Neither herbal cake-partitioned moxibustion nor mild moxibustion influences the colonic histopathology and intestinal mucosal barrier-related protein expression in the normal rats;both herbal cake-partitioned moxibustion and mild moxibustion can up-regulate the protein expression levels of JAM1,MUC2,and TGF-β1 in the colon tissue of UC rats.Mild moxibustion can up-regulate Occludin protein expression.This may be a mechanism of moxibustion in reducing colonic mucosa inflammation in UC.
