目的利用近红外光谱及光谱融合策略,结合化学计量学方法,建立水性油墨的颜色预测模型,实现水性油墨印刷品颜色准确预测。方法采集不同酒精含量和不同调色墨含量的油墨的近红外光谱反射率和吸光度数据,并测得对应的印刷品的Lab值,然后建...目的利用近红外光谱及光谱融合策略,结合化学计量学方法,建立水性油墨的颜色预测模型,实现水性油墨印刷品颜色准确预测。方法采集不同酒精含量和不同调色墨含量的油墨的近红外光谱反射率和吸光度数据,并测得对应的印刷品的Lab值,然后建立单一光谱不同预处理过后的偏最小二乘(Partial Least Squares,PLS)模型,以及基于数据层融合和特征层融合的PLS模型,最终通过比较预测集决定系数和预测集均方根误差(Root Mean Square Error of Prediction,RMSEP)以及色差来评估模型的预测效果。结果单光谱建模,基于反射率建立的模型准确率高于基于吸光度建立的模型;数据层融合缺乏稳定性,对L和b值的预测有所提升,对a值的预测几乎不变;特征层融合建模效果明显好于单一光谱和数据层融合,对Lab的预测决定系数分别达到了0.9961、0.9939、0.9974;RMSEP值分别为0.1421、0.2126、0.2072;预测值与真实值的最大色差为0.6783。结论通过光谱特征融合技术能提高油墨颜色预测精度,准确预测出油墨颜色变化。展开更多
目的:探究多维度强化结合阶段性康复训练对骨折患者康复及静脉血栓栓塞症发生的影响。方法:选取景德镇市第三人民医院于2020年1月—2023年3月收治的下肢骨折患者88例,根据单双数标记法,将单数归为对照组,双数归为观察组,各44例。对照组...目的:探究多维度强化结合阶段性康复训练对骨折患者康复及静脉血栓栓塞症发生的影响。方法:选取景德镇市第三人民医院于2020年1月—2023年3月收治的下肢骨折患者88例,根据单双数标记法,将单数归为对照组,双数归为观察组,各44例。对照组采用常规护理干预,观察组采用多维度强化结合阶段性康复训练,干预周期为6个月。比较两组患者干预疗效(住院时间、下床活动时间、骨折愈合时间)、疼痛程度[视觉模拟评分法(visual analog scale,VAS)评分]、自护能力[自我护理能力量表(exercise of self-care ability,ESCA)]、下肢运动功能恢复情况[简式下肢Fugl-Meyer运动评估表(Fugl-Meyer assessment scale,FMA)和Berg平衡量表(Berg balance scale,BBS)]及静脉血栓栓塞症(VTE)发生率。结果:干预后,两组患者VAS评分均低于干预前,ESCA各维度评分、FMA评分及BBS评分均高于干预前,差异均有统计学意义(P<0.05);观察组住院时间、骨折愈合时间均短于对照组,下床活动时间早于对照组,VAS评分低于对照组,ESCA各维度评分、FMA评分、BBS评分均高于对照组,差异均有统计学意义(P<0.05)。干预6个月,观察组VTE发生率低于对照组(P<0.05)。结论:多维度强化结合阶段性康复训练能显著缩短下肢骨折患者的住院和恢复时间,降低疼痛程度,提高下肢运动功能和自护能力,并有效减少VTE的发生。展开更多
目的:探讨木香烃内酯(costunolide,Cos)对人肝内胆管癌RBE细胞生物学行为的影响及其作用机制。方法:以不同质量浓度(0、2、4、6、8、12、16、20μg/ml)Cos作用RBE细胞,通过CCK-8法、流式细胞术分别检测Cos对细胞增殖能力和周期分布的影...目的:探讨木香烃内酯(costunolide,Cos)对人肝内胆管癌RBE细胞生物学行为的影响及其作用机制。方法:以不同质量浓度(0、2、4、6、8、12、16、20μg/ml)Cos作用RBE细胞,通过CCK-8法、流式细胞术分别检测Cos对细胞增殖能力和周期分布的影响,Annexin V-FITC/PI双染法、Transwell小室实验分别检测Cos对细胞凋亡和迁移侵袭的影响,q RT-PCR检测与侵袭相关因子MMP2、MMP9 m RNA的表达,Western blotting检测PI3K/AKT信号通路相关蛋白的表达。结果:Cos能够显著抑制RBE细胞的增殖活性(P<0.05或P<0.01),阻滞细胞周期于S、G2/M期,同时诱导RBE细胞的凋亡(P<0.01)、且呈质量浓度依赖性,Transwell及q RT-PCR结果显示Cos能够显著抑制RBE细胞的侵袭能力、降低侵袭相关转录因子MMP2和MMP9 m RNA的表达,Western blotting结果显示Cos明显抑制p-AKT、Bcl-2、MMP2及MMP9的表达,但促进Bax的表达。结论:Cos通过抑制PI3K/AKT信号通路降低胆管癌RBE细胞的增殖、迁移及侵袭能力。展开更多
Hepatocellular carcinoma is a highly malignant neoplasm and frequently involves extrahepatic organs but decidedly rarely occurs in brain. We describe 3 cases of brain metastases in patients suffering from post-HBV hep...Hepatocellular carcinoma is a highly malignant neoplasm and frequently involves extrahepatic organs but decidedly rarely occurs in brain. We describe 3 cases of brain metastases in patients suffering from post-HBV hepatocarcinoma. The ＂stroke-like＂ presentation of the cerebral localization of the disease can be explained by both the important vascularization of the tumor and the frequent hemocoagulative alterations caused by the cirrhosis. The importance of diagnostic neuroradiology is briefly addressed, with reference to the fundamental role played by MRI. Surgery of these lesions does not present any particular technical problems as long as they are located in accessible areas and the patient＇s general and neurological conditions allow it. Postoperative radiotherapy seems to improve the quality and quantity of residual life, although the number of patients described in the literature was too small to draw any definite conclusion in this regard.展开更多
Objective:Percutaneous radiofrequency ablation(PRFA) is known to be as effective as hepatectomy for small hepatocellular carcinoma(HCC) in the long-term.We wished to ascertain how it is for recurrent small HCC.Methods...Objective:Percutaneous radiofrequency ablation(PRFA) is known to be as effective as hepatectomy for small hepatocellular carcinoma(HCC) in the long-term.We wished to ascertain how it is for recurrent small HCC.Methods:From January 2009 to November 2011,a series of sixty-one patients were included in the study according to the criteria:each patient had one recurrent HCC,less than 5 cm in diameter.Twenty-six of the 61 patients were treated with PRFA and the other 35 were treated with repeat hepatectomy.Results:The interval from first surgery to recurrent for repeat hepatectomy and PRFA groups were(1,239.60±1,017.00) d and(903.42±975.11) d respectively(P=0.066).The tumor-free time after repeat hepatectomy and PRFA were(310.23±159.50) d and(278.27±123.29) d respectively(P=0.584).Size of tumor in repeat hepatectomy and PRFA were(7.34±3.16) cm^2 and(5.59±3.40) cm^2(P=0.215),the total expenditure for each patient of the two groups were(26,150.66±7,923.60) yuan and(21,135.00±1,156.76) yuan(RMB),and the time of hospitalization for each of the two groups were(15.29±4.28) d and(7.46±2.20) d(P<0.001).Conclusion:PRFA is proved to be as effective as repeat hepatectomy in the treatment of recurrent small HCC,and superior to repeat hepatectomy as it is less invasive.展开更多
Objective： To summarize the experience of diagnosis and surgical treatment for a rare invasion manner of gallbladder carcinoma, which was combined with tumor thrombus in common bile duct （CBD）. Methods： Between Fe...Objective： To summarize the experience of diagnosis and surgical treatment for a rare invasion manner of gallbladder carcinoma, which was combined with tumor thrombus in common bile duct （CBD）. Methods： Between February 2003 and January 2005, 3 patients with gallbladder carcinoma who were identified of tumor thrombus in common bile duct in surgical procedure were retrospectively analyzed. Results： Abdominal ultrasound and magnetic resonance cholangiopancreatography （MRCP） were used for preoperative diagnosis. All 3 patients were given radical operations, which were composed of cholecystectomy, resection of the extrahepatic biliary duct, cuniform hepatectomy of gallbladder bed, skeletonization of the hepatoduodenal ligament, hilar choledochojejunostomy, and clearance of tumor thrombus from bile duct. Three patients were recovered well after surgery, which were respectively alive for 30 months, 17 months and 23 months without tumor recurrence,and 58 months, 41 months and 40 months for survival time after operation. Conclusion： Gallbladder carcinoma with tumor thrombus in common bile duct was very rare but with relatively special clinical manifestation and characteristic radiography manifestation. MRCP was one of the most potent diagnostic method. The prognosis of gallbladder carcinoma with tumor thrombus in common bile duct after surgical procedure was apparently better than gallbladder carcinoma with invasion of hilar tissues. Radical operation was feasible and safe for obtaining longer survival展开更多
PCP-2 is a member of receptor-like protein tyrosine phosphatase of the MAM do-main family.To investigate which part of PCP-2 was involved in its interaction withβ-catenin,we constructed various deletion mutants of PC...PCP-2 is a member of receptor-like protein tyrosine phosphatase of the MAM do-main family.To investigate which part of PCP-2 was involved in its interaction withβ-catenin,we constructed various deletion mutants of PCP-2.These PCP-2 mutants and wild-type PCP-2 were co-transfected into BHK-21 cells withβ-catenin individually.An in vivo binding assay revealed that the expression of wild-type PCP-2,PCP-2?C1C2(deleted PCP-2 without both PTP domains)and PCP-2?C2(deleted PCP-2 without the second PTP domain)could be immunoprecipitated by anti-catenin antibody in every co-transfection,but PCP-2 EXT(deleted PCP-2 without the juxtamembrane region and both PTP domains)was missing,which implied that PCP-2 andβ-catenin could associate directly and the juxtamembrane region in PCP-2 was sufficient for the process.展开更多
文摘目的利用近红外光谱及光谱融合策略,结合化学计量学方法,建立水性油墨的颜色预测模型,实现水性油墨印刷品颜色准确预测。方法采集不同酒精含量和不同调色墨含量的油墨的近红外光谱反射率和吸光度数据,并测得对应的印刷品的Lab值,然后建立单一光谱不同预处理过后的偏最小二乘(Partial Least Squares,PLS)模型,以及基于数据层融合和特征层融合的PLS模型,最终通过比较预测集决定系数和预测集均方根误差(Root Mean Square Error of Prediction,RMSEP)以及色差来评估模型的预测效果。结果单光谱建模,基于反射率建立的模型准确率高于基于吸光度建立的模型;数据层融合缺乏稳定性,对L和b值的预测有所提升,对a值的预测几乎不变;特征层融合建模效果明显好于单一光谱和数据层融合,对Lab的预测决定系数分别达到了0.9961、0.9939、0.9974;RMSEP值分别为0.1421、0.2126、0.2072;预测值与真实值的最大色差为0.6783。结论通过光谱特征融合技术能提高油墨颜色预测精度,准确预测出油墨颜色变化。
文摘目的:探究多维度强化结合阶段性康复训练对骨折患者康复及静脉血栓栓塞症发生的影响。方法:选取景德镇市第三人民医院于2020年1月—2023年3月收治的下肢骨折患者88例,根据单双数标记法,将单数归为对照组,双数归为观察组,各44例。对照组采用常规护理干预,观察组采用多维度强化结合阶段性康复训练,干预周期为6个月。比较两组患者干预疗效(住院时间、下床活动时间、骨折愈合时间)、疼痛程度[视觉模拟评分法(visual analog scale,VAS)评分]、自护能力[自我护理能力量表(exercise of self-care ability,ESCA)]、下肢运动功能恢复情况[简式下肢Fugl-Meyer运动评估表(Fugl-Meyer assessment scale,FMA)和Berg平衡量表(Berg balance scale,BBS)]及静脉血栓栓塞症(VTE)发生率。结果:干预后,两组患者VAS评分均低于干预前,ESCA各维度评分、FMA评分及BBS评分均高于干预前,差异均有统计学意义(P<0.05);观察组住院时间、骨折愈合时间均短于对照组,下床活动时间早于对照组,VAS评分低于对照组,ESCA各维度评分、FMA评分、BBS评分均高于对照组,差异均有统计学意义(P<0.05)。干预6个月,观察组VTE发生率低于对照组(P<0.05)。结论:多维度强化结合阶段性康复训练能显著缩短下肢骨折患者的住院和恢复时间,降低疼痛程度,提高下肢运动功能和自护能力,并有效减少VTE的发生。
文摘目的:探讨木香烃内酯(costunolide,Cos)对人肝内胆管癌RBE细胞生物学行为的影响及其作用机制。方法:以不同质量浓度(0、2、4、6、8、12、16、20μg/ml)Cos作用RBE细胞,通过CCK-8法、流式细胞术分别检测Cos对细胞增殖能力和周期分布的影响,Annexin V-FITC/PI双染法、Transwell小室实验分别检测Cos对细胞凋亡和迁移侵袭的影响,q RT-PCR检测与侵袭相关因子MMP2、MMP9 m RNA的表达,Western blotting检测PI3K/AKT信号通路相关蛋白的表达。结果:Cos能够显著抑制RBE细胞的增殖活性(P<0.05或P<0.01),阻滞细胞周期于S、G2/M期,同时诱导RBE细胞的凋亡(P<0.01)、且呈质量浓度依赖性,Transwell及q RT-PCR结果显示Cos能够显著抑制RBE细胞的侵袭能力、降低侵袭相关转录因子MMP2和MMP9 m RNA的表达,Western blotting结果显示Cos明显抑制p-AKT、Bcl-2、MMP2及MMP9的表达,但促进Bax的表达。结论:Cos通过抑制PI3K/AKT信号通路降低胆管癌RBE细胞的增殖、迁移及侵袭能力。
文摘Hepatocellular carcinoma is a highly malignant neoplasm and frequently involves extrahepatic organs but decidedly rarely occurs in brain. We describe 3 cases of brain metastases in patients suffering from post-HBV hepatocarcinoma. The ＂stroke-like＂ presentation of the cerebral localization of the disease can be explained by both the important vascularization of the tumor and the frequent hemocoagulative alterations caused by the cirrhosis. The importance of diagnostic neuroradiology is briefly addressed, with reference to the fundamental role played by MRI. Surgery of these lesions does not present any particular technical problems as long as they are located in accessible areas and the patient＇s general and neurological conditions allow it. Postoperative radiotherapy seems to improve the quality and quantity of residual life, although the number of patients described in the literature was too small to draw any definite conclusion in this regard.
基金Supported by the grant of Ministry of Science and Technology of the People' s Republic of China(2008ZX10002-25)National Natural Science Foundation of China(81070359)
文摘Objective:Percutaneous radiofrequency ablation(PRFA) is known to be as effective as hepatectomy for small hepatocellular carcinoma(HCC) in the long-term.We wished to ascertain how it is for recurrent small HCC.Methods:From January 2009 to November 2011,a series of sixty-one patients were included in the study according to the criteria:each patient had one recurrent HCC,less than 5 cm in diameter.Twenty-six of the 61 patients were treated with PRFA and the other 35 were treated with repeat hepatectomy.Results:The interval from first surgery to recurrent for repeat hepatectomy and PRFA groups were(1,239.60±1,017.00) d and(903.42±975.11) d respectively(P=0.066).The tumor-free time after repeat hepatectomy and PRFA were(310.23±159.50) d and(278.27±123.29) d respectively(P=0.584).Size of tumor in repeat hepatectomy and PRFA were(7.34±3.16) cm^2 and(5.59±3.40) cm^2(P=0.215),the total expenditure for each patient of the two groups were(26,150.66±7,923.60) yuan and(21,135.00±1,156.76) yuan(RMB),and the time of hospitalization for each of the two groups were(15.29±4.28) d and(7.46±2.20) d(P<0.001).Conclusion:PRFA is proved to be as effective as repeat hepatectomy in the treatment of recurrent small HCC,and superior to repeat hepatectomy as it is less invasive.
文摘Objective： To summarize the experience of diagnosis and surgical treatment for a rare invasion manner of gallbladder carcinoma, which was combined with tumor thrombus in common bile duct （CBD）. Methods： Between February 2003 and January 2005, 3 patients with gallbladder carcinoma who were identified of tumor thrombus in common bile duct in surgical procedure were retrospectively analyzed. Results： Abdominal ultrasound and magnetic resonance cholangiopancreatography （MRCP） were used for preoperative diagnosis. All 3 patients were given radical operations, which were composed of cholecystectomy, resection of the extrahepatic biliary duct, cuniform hepatectomy of gallbladder bed, skeletonization of the hepatoduodenal ligament, hilar choledochojejunostomy, and clearance of tumor thrombus from bile duct. Three patients were recovered well after surgery, which were respectively alive for 30 months, 17 months and 23 months without tumor recurrence,and 58 months, 41 months and 40 months for survival time after operation. Conclusion： Gallbladder carcinoma with tumor thrombus in common bile duct was very rare but with relatively special clinical manifestation and characteristic radiography manifestation. MRCP was one of the most potent diagnostic method. The prognosis of gallbladder carcinoma with tumor thrombus in common bile duct after surgical procedure was apparently better than gallbladder carcinoma with invasion of hilar tissues. Radical operation was feasible and safe for obtaining longer survival
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.30270686 and 30370740)the Key Research Project(30110811 and 03DJ14007).
文摘PCP-2 is a member of receptor-like protein tyrosine phosphatase of the MAM do-main family.To investigate which part of PCP-2 was involved in its interaction withβ-catenin,we constructed various deletion mutants of PCP-2.These PCP-2 mutants and wild-type PCP-2 were co-transfected into BHK-21 cells withβ-catenin individually.An in vivo binding assay revealed that the expression of wild-type PCP-2,PCP-2?C1C2(deleted PCP-2 without both PTP domains)and PCP-2?C2(deleted PCP-2 without the second PTP domain)could be immunoprecipitated by anti-catenin antibody in every co-transfection,but PCP-2 EXT(deleted PCP-2 without the juxtamembrane region and both PTP domains)was missing,which implied that PCP-2 andβ-catenin could associate directly and the juxtamembrane region in PCP-2 was sufficient for the process.
