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加味天麻温胆汤对老年高血压患者免疫功能、C反应蛋白及同型半胱氨酸的影响 被引量:1
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作者 余茂强 吴绍长 +3 位作者 王雅莉 陶剑虹 朱满连 刘小菊 《云南中医中药杂志》 2018年第9期29-31,共3页
目的观察加味天麻温胆汤对老年高血压患者免疫功能、C反应蛋白及同型半胱氨酸的影响。方法将符合标准的60例患者采用分层区组随机法分组,分为对照组、治疗组各30例,对照组每日口服厄贝沙坦氢氯噻嗪片1片,治疗组在对照组的基础上每日口... 目的观察加味天麻温胆汤对老年高血压患者免疫功能、C反应蛋白及同型半胱氨酸的影响。方法将符合标准的60例患者采用分层区组随机法分组,分为对照组、治疗组各30例,对照组每日口服厄贝沙坦氢氯噻嗪片1片,治疗组在对照组的基础上每日口服加味天麻温胆汤1剂。总疗程均为28 d。观察治疗前后患者免疫功能、C反应蛋白及同型半胱酸的变化。结果治疗后治疗组免疫功能、CRP、CRP、hs-CRP、同型半胱氨酸水平均低于对照组(P <0. 05)。结论加味天麻温胆汤可改善患者免疫炎症反应、降低同型半胱氨酸水平,具有延缓高血压患者靶器官的损害。 展开更多
关键词 加味天麻温胆汤 老年高血压 免疫功能 C反应蛋白 同型半胱酸 临床观察
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液质联用方法用于多发性硬化的血浆蛋白质组标志物研究
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作者 杨雯棋 吴绍长 +6 位作者 周大金 章丽娟 闫秀梅 何成彦 何平 孙景春 赵龙友 《中国实验诊断学》 2021年第10期1532-1535,共4页
目的通过血浆蛋白质组液质联用分析寻找多发性硬化疾病中差异表达蛋白,寻找多发性硬化早期诊断相关的血浆标志物。方法选取多发性硬化患者和健康对照人各9例,采用10标TMT标记技术进行血浆蛋白质组分析,筛选差异蛋白。结果液质联用血浆... 目的通过血浆蛋白质组液质联用分析寻找多发性硬化疾病中差异表达蛋白,寻找多发性硬化早期诊断相关的血浆标志物。方法选取多发性硬化患者和健康对照人各9例,采用10标TMT标记技术进行血浆蛋白质组分析,筛选差异蛋白。结果液质联用血浆蛋白质组差异分析共鉴定到52个差异蛋白质,其中上调27个,下调25个。差异蛋白质功能分析显示,这些蛋白与免疫系统、补体系统、急性期反应信号等通路相关。结论在血浆蛋白质组中可以找到与多发性硬化相关的蛋白质,这些有可能成为其早期诊断的标志物。本研究为血浆蛋白质组学在多发性硬化诊断中的进一步应用奠定了基础。 展开更多
关键词 液质联用技术 多发性硬化 血浆 蛋白质组
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抗人TDP-43单克隆抗体的制备纯化及效价测定
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作者 吴绍长 刘奕 申子好 《生物学杂志》 CAS CSCD 北大核心 2018年第4期103-105,共3页
为了建立人TDP-43蛋白的检测、纯化方法以及为开发相关的检测试剂提供基础,利用重组TDP-43免疫BALB/c小鼠,采用杂交瘤细胞技术,获得分泌抗人TDP-43蛋白抗体的杂交瘤细胞株,用杂交瘤细胞株诱生小鼠腹水,应用蛋白A亲和层析法进行抗体纯化... 为了建立人TDP-43蛋白的检测、纯化方法以及为开发相关的检测试剂提供基础,利用重组TDP-43免疫BALB/c小鼠,采用杂交瘤细胞技术,获得分泌抗人TDP-43蛋白抗体的杂交瘤细胞株,用杂交瘤细胞株诱生小鼠腹水,应用蛋白A亲和层析法进行抗体纯化,并采用ELISA测定抗体效价。结果表明,2株稳定分泌抗人TDP-43单克隆抗体细胞株1C5和2A5被成功制备,其亚类均为Ig G1,效价分别为1∶256 000和1∶512 000。 展开更多
关键词 TDP-43 单克隆抗体 制备 鉴定
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Clinical observation on acupoint injection for back pain in patients with primary osteoporosis 被引量:3
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作者 Hua Ying Wang Yan wu shao-chang 《Journal of Acupuncture and Tuina Science》 CSCD 2020年第5期379-383,共5页
Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided int... Objective:To observe the clinical efficacy of acupoint injection with salmon calcitonin for back pain in elderly patients with primary osteoporosis.Methods:A total of 76 patients were selected and randomly divided into two groups by the random number table method,with 39 cases in the treatment group and 37 cases in the control group,respectively.Patients in both groups received routine anti-osteoporosis treatment.Patients in the treatment group received additional acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23),while patients in the control group received additional intramuscular injection with salmon calcitonin.The treatments for both groups were given once a day and lasted for 4 weeks.Visual analog scale(VAS)and Chinese Oswestry disability index(CODI)scores were observed before treatment,after 2 weeks and 4 weeks of treatment,and the use of analgesics during the treatment were recorded.Results:After treatment,the clinical efficacy in the treatment group was more significant than that in the control group(P<0.05).After 2 weeks and 4 weeks of treatment,the VAS scores in both groups showed significant intra-group and between-group differences(all P<0.05),and the CODI scores in both groups showed significant intra-group differences(all P<0.05).After 2 weeks of treatment,the CODI score showed no significant between-group difference(P>0.05).After 4 weeks of treatment,the improvement of CODI score in the treatment group was more significant than that in the control group(P<0.05).During the treatment,2 cases in the treatment group took analgesics versus 8 cases in the control group,and the result showed a significant between-group difference(P<0.05).Conclusion:For back pain in elderly patients with primary osteoporosis,based on the routine treatment of oral medication,the clinical efficacy of acupoint injection with salmon calcitonin at bilateral Pishu(BL 20)and Shenshu(BL 23)is more significant than that of intramuscular injection.Acupoint injection treatment can improve patients'conditions and reduce the use of analgesics. 展开更多
关键词 Acupoint Therapy HYDRO-ACUPUNCTURE Point Pishu(BL 20) Point Shenshu(BL 23) Visual Analog Scale Osteoporosis Low Back Pain Aged
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