The in vitro and in vivo anti-tumor efficacy of methotrexate-loaded Fe3O4-poly-L-lactide-poly(ethylene glycol)-poly-L-lactide magnetic composite microspheres(MTX-Fe3O4-PLLA-PEG-PLLA MCMs,MMCMs),which were produced by ...The in vitro and in vivo anti-tumor efficacy of methotrexate-loaded Fe3O4-poly-L-lactide-poly(ethylene glycol)-poly-L-lactide magnetic composite microspheres(MTX-Fe3O4-PLLA-PEG-PLLA MCMs,MMCMs),which were produced by co-precipitation(C)and microencapsulation(M)in a supercritical process,was evaluated at various levels:cellular,molecular,and integrated.The results at the cellular level indicate that MMCMs(M)show a better anti-proliferation activity than raw MTX and could induce morphological changes of cells undergoing apoptosis.At the molecular level,MMCMs(M)lead to a significantly higher relative mRNA expression of bax/bcl-2 and caspase-3 than MMCMs(C)at 10μg mL-1(P<0.01);and the pro-caspase-3protein expression measured by Western blot analysis also demonstrates that MMCMs(M)can effectively activate pro-caspase-3.At the integrated level,mice bearing a sarcoma-180 tumor are used;in vivo anti-tumor activity tests reveal that MMCMs(M)with magnetic induction display a much higher tumor suppression rate and lower toxicity than raw MTX.Pharmacokinetic studies show that MMCMs(M)with magnetic induction significantly increase the accumulation of MTX in the tumor tissue compared with the other treatments.These results suggest that the MMCMs(M)prepared by the SpEDS process have great potential to play a positive role in the magnetic targeted therapy field.展开更多
OBJECTIVE:To assess the efficacy of acupoint therapy combined with spine pinching in patients with menopausal syndrome.METHODS:This is a parallel,randomized,controlled,investigator-blinded trial.A total of 132 partici...OBJECTIVE:To assess the efficacy of acupoint therapy combined with spine pinching in patients with menopausal syndrome.METHODS:This is a parallel,randomized,controlled,investigator-blinded trial.A total of 132 participants were randomly assigned to receive either acupoint therapy combined with spine pinching(intervention group)or tibolone therapy alone(control group).The intervention group received acupoint therapy combined with spine pinching three times per week for 4 weeks.The control group received 2.5 mg of tibolone once daily for 4 weeks.The primary outcome was the improved Kupperman score.The WHO quality of life scale was also used.The secondary aim was to identify those who would benefit from acupoint therapy combined with spine pinching based on the levels of follicle stimulating hormone(FSH)and luteinizing hormone(LH).RESULTS:In the intervention group,the improved Kupperman score was significantly decreased after treatment compared with before treatment.However,there were no differences between the intervention and control groups for any outcome.Changes in the physiology score presented negative outcomes in patients with a low FSH level with increasing body mass index(BMI)(P=0.0).In contrast,changes in the physiology score presented positive outcomes in patients with a moderate LH level with increasing BMI(P=0.0).The mean change in the physiology score of patients with a low FSH level and a BMI of≥25.7 kg/m2 was-7.17(range-10.94 to-3.40)after adjustments for age and disease duration.CONCLUSION:Acupoint therapy combined with spine pinching is effective in treating menopausal syndrome,especially in women with a moderate LH level.However,patients with a low FSH level had a negative outcome after acupoint therapy combined with spine pinching.In addition,patients with a BMI of>25.7 kg/m2 had a negative outcome after the intervention,regardless of hormone levels.展开更多
基金supported by the National Natural Science Foundation of China (51103049,31170939)Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University (ZQN-PY107)+1 种基金Science and Technology Project of Fujian Province (2013Y2002)Specialized Research Fund for the Doctoral Program of Higher Education (20133501110003)
文摘The in vitro and in vivo anti-tumor efficacy of methotrexate-loaded Fe3O4-poly-L-lactide-poly(ethylene glycol)-poly-L-lactide magnetic composite microspheres(MTX-Fe3O4-PLLA-PEG-PLLA MCMs,MMCMs),which were produced by co-precipitation(C)and microencapsulation(M)in a supercritical process,was evaluated at various levels:cellular,molecular,and integrated.The results at the cellular level indicate that MMCMs(M)show a better anti-proliferation activity than raw MTX and could induce morphological changes of cells undergoing apoptosis.At the molecular level,MMCMs(M)lead to a significantly higher relative mRNA expression of bax/bcl-2 and caspase-3 than MMCMs(C)at 10μg mL-1(P<0.01);and the pro-caspase-3protein expression measured by Western blot analysis also demonstrates that MMCMs(M)can effectively activate pro-caspase-3.At the integrated level,mice bearing a sarcoma-180 tumor are used;in vivo anti-tumor activity tests reveal that MMCMs(M)with magnetic induction display a much higher tumor suppression rate and lower toxicity than raw MTX.Pharmacokinetic studies show that MMCMs(M)with magnetic induction significantly increase the accumulation of MTX in the tumor tissue compared with the other treatments.These results suggest that the MMCMs(M)prepared by the SpEDS process have great potential to play a positive role in the magnetic targeted therapy field.
基金Supported by the Special Project for Scientific Research and Construction of a National Traditional Chinese Medicine Clinical Research Base of the China State Administration of Traditional Chinese Medicine(No.JD2X2012019)
文摘OBJECTIVE:To assess the efficacy of acupoint therapy combined with spine pinching in patients with menopausal syndrome.METHODS:This is a parallel,randomized,controlled,investigator-blinded trial.A total of 132 participants were randomly assigned to receive either acupoint therapy combined with spine pinching(intervention group)or tibolone therapy alone(control group).The intervention group received acupoint therapy combined with spine pinching three times per week for 4 weeks.The control group received 2.5 mg of tibolone once daily for 4 weeks.The primary outcome was the improved Kupperman score.The WHO quality of life scale was also used.The secondary aim was to identify those who would benefit from acupoint therapy combined with spine pinching based on the levels of follicle stimulating hormone(FSH)and luteinizing hormone(LH).RESULTS:In the intervention group,the improved Kupperman score was significantly decreased after treatment compared with before treatment.However,there were no differences between the intervention and control groups for any outcome.Changes in the physiology score presented negative outcomes in patients with a low FSH level with increasing body mass index(BMI)(P=0.0).In contrast,changes in the physiology score presented positive outcomes in patients with a moderate LH level with increasing BMI(P=0.0).The mean change in the physiology score of patients with a low FSH level and a BMI of≥25.7 kg/m2 was-7.17(range-10.94 to-3.40)after adjustments for age and disease duration.CONCLUSION:Acupoint therapy combined with spine pinching is effective in treating menopausal syndrome,especially in women with a moderate LH level.However,patients with a low FSH level had a negative outcome after acupoint therapy combined with spine pinching.In addition,patients with a BMI of>25.7 kg/m2 had a negative outcome after the intervention,regardless of hormone levels.
