目的建立基于共价有机聚合物固相微萃取前处理结合高效液相色谱-串联质谱法(highperformance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)快速、高灵敏检测牛奶中6种黄曲霉毒素残留的分析方法。方法以1,3,5-三(4-氨苯...目的建立基于共价有机聚合物固相微萃取前处理结合高效液相色谱-串联质谱法(highperformance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)快速、高灵敏检测牛奶中6种黄曲霉毒素残留的分析方法。方法以1,3,5-三(4-氨苯基)苯[1,3,5-tri(4-aminophenyl)benzene,TAPB]和1,3,5-三甲酰基间苯三酚(1,3,5-triformyl-phloroglucinol,Tp)为单体制备了共价有机聚合物材料并固定在木签表面,用于黄曲霉毒素的固相微萃取前处理。在萃取时间为20 min、洗脱溶剂为乙腈、洗脱时间为6 min条件下,对黄曲霉毒素进行萃取和洗脱。结果6种黄曲霉毒素在一定质量浓度范围内线性关系良好,相关系数均大于0.9995。日内和日间精密度的相对标准偏差(relative standard deviations,RSDs)分别为4.39%~8.21%和4.97%~9.12%。将开发的方法用于实际牛奶样品中黄曲霉毒素残留检测,加标回收率为80.01%~92.71%,RSDs为3.12%~9.54%。结论制备的共价有机聚合物固相微萃取材料对6种黄曲霉毒素吸附性能强,开发的固相微萃取-HPLC-MS/MS适用于牛奶中黄曲霉毒素的快速、高灵敏定量检测。展开更多
Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear...Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear.Here,we identified a novel antisense transcript,named APMAP-AS,transcribed from adipocyte membrane-associated protein(APMAP)in the pig genome.APMAP-AS and APMAP were highly expressed in retroperitoneal adipose of obese pigs,compared with that in control pigs.Using a bone mesenchymal stem cells(BMSCs)adipogenic differentiation model,we found that APMAP-AS positively regulated adipogenic differentiation.APMAP-AS had the potential to form an RNA–RNA duplex with APMAP,and increased the stability of APMAP mRNA.Additionally,APMAP-AS promoted lipid metabolism and inhibited the expression of inflammatory factors.These findings of a natural antisense transcript for a regulatory gene associated with lipid synthesis might further our understanding of lncRNAs in driving adaptive adipose tissue remodeling and preserving metabolic health.展开更多
目的·探讨双相障碍患者攻击行为与多巴胺D4受体(dopamine D4 receptor,DRD4)基因第3外显子(exonⅢ)48 bp可变数目串联重复序列(variable number tandem repeat,VNTR)多态性及人格特征之间的关系。方法·选取2015年1月-2018年1...目的·探讨双相障碍患者攻击行为与多巴胺D4受体(dopamine D4 receptor,DRD4)基因第3外显子(exonⅢ)48 bp可变数目串联重复序列(variable number tandem repeat,VNTR)多态性及人格特征之间的关系。方法·选取2015年1月-2018年12月在上海市长宁区精神卫生中心、上海市静安区精神卫生中心、上海市徐汇区精神卫生中心和上海健康医学院附属嘉定区中心医院就诊的双相障碍患者共173例,根据修订版外显攻击行为量表(Modified Overt Aggression Scale,MOAS)评分将其分为攻击组(研究组)和非攻击组(对照组)。分别进行一般情况调查、气质性格问卷(Temperature and Character Inventory,TCI)测定;采用聚合酶链反应-限制性片断长度多态性技术检测DRD4 exonⅢ48 bp VNTR多态性位点。用SHEsis软件进行基因型Hardy-Weinberg遗传平衡度检验及组间基因型频率的差异分析,用χ~2检验和t检验分析双相障碍患者攻击行为与DRD4基因多态性及人格特征之间的关系。结果·研究组和对照组一般情况调查结果间差异无统计学意义(P>0.05)。共检测到DRD4 exonⅢ48 bp VNTR的6种等位基因和9种基因型,2组最常见的是4次重复等位基因。2组间DRD4基因的基因型及等位基因的频率分布差异有统计学意义(P=0.040,P=0.018)。研究组寻求新奇、伤害避免维度评分高于对照组(P=0.026,P=0.000),合作性、自我定向维度评分低于对照组(均P=0.000)。研究组携带长重复等位基因的患者寻求新奇、伤害避免维度评分高于携带短重复等位基因的患者(P=0.000,P=0.006),合作性维度评分低于携带短重复等位基因的患者(P=0.038)。结论·双相障碍患者攻击行为与DRD4 exonⅢ48 bp VNTR之间可能存在关联。双相障碍攻击行为患者具有冲动、寻求刺激、探索、害怕不确定、报复心强、自私自利等人格特征,携带长重复等位基因患者人格异常更显著。展开更多
文摘目的建立基于共价有机聚合物固相微萃取前处理结合高效液相色谱-串联质谱法(highperformance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)快速、高灵敏检测牛奶中6种黄曲霉毒素残留的分析方法。方法以1,3,5-三(4-氨苯基)苯[1,3,5-tri(4-aminophenyl)benzene,TAPB]和1,3,5-三甲酰基间苯三酚(1,3,5-triformyl-phloroglucinol,Tp)为单体制备了共价有机聚合物材料并固定在木签表面,用于黄曲霉毒素的固相微萃取前处理。在萃取时间为20 min、洗脱溶剂为乙腈、洗脱时间为6 min条件下,对黄曲霉毒素进行萃取和洗脱。结果6种黄曲霉毒素在一定质量浓度范围内线性关系良好,相关系数均大于0.9995。日内和日间精密度的相对标准偏差(relative standard deviations,RSDs)分别为4.39%~8.21%和4.97%~9.12%。将开发的方法用于实际牛奶样品中黄曲霉毒素残留检测,加标回收率为80.01%~92.71%,RSDs为3.12%~9.54%。结论制备的共价有机聚合物固相微萃取材料对6种黄曲霉毒素吸附性能强,开发的固相微萃取-HPLC-MS/MS适用于牛奶中黄曲霉毒素的快速、高灵敏定量检测。
基金supported by grants from the National Key R&D Program of China(2021YFA0805903,2020YFA0509500 and 2021YFD1300800)the Sichuan Science and Technology Program,China(2021ZDZX0008,2021YFYZ0009,2021YFYZ0030 and 2022JDJQ0054).
文摘Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear.Here,we identified a novel antisense transcript,named APMAP-AS,transcribed from adipocyte membrane-associated protein(APMAP)in the pig genome.APMAP-AS and APMAP were highly expressed in retroperitoneal adipose of obese pigs,compared with that in control pigs.Using a bone mesenchymal stem cells(BMSCs)adipogenic differentiation model,we found that APMAP-AS positively regulated adipogenic differentiation.APMAP-AS had the potential to form an RNA–RNA duplex with APMAP,and increased the stability of APMAP mRNA.Additionally,APMAP-AS promoted lipid metabolism and inhibited the expression of inflammatory factors.These findings of a natural antisense transcript for a regulatory gene associated with lipid synthesis might further our understanding of lncRNAs in driving adaptive adipose tissue remodeling and preserving metabolic health.
文摘目的·探讨双相障碍患者攻击行为与多巴胺D4受体(dopamine D4 receptor,DRD4)基因第3外显子(exonⅢ)48 bp可变数目串联重复序列(variable number tandem repeat,VNTR)多态性及人格特征之间的关系。方法·选取2015年1月-2018年12月在上海市长宁区精神卫生中心、上海市静安区精神卫生中心、上海市徐汇区精神卫生中心和上海健康医学院附属嘉定区中心医院就诊的双相障碍患者共173例,根据修订版外显攻击行为量表(Modified Overt Aggression Scale,MOAS)评分将其分为攻击组(研究组)和非攻击组(对照组)。分别进行一般情况调查、气质性格问卷(Temperature and Character Inventory,TCI)测定;采用聚合酶链反应-限制性片断长度多态性技术检测DRD4 exonⅢ48 bp VNTR多态性位点。用SHEsis软件进行基因型Hardy-Weinberg遗传平衡度检验及组间基因型频率的差异分析,用χ~2检验和t检验分析双相障碍患者攻击行为与DRD4基因多态性及人格特征之间的关系。结果·研究组和对照组一般情况调查结果间差异无统计学意义(P>0.05)。共检测到DRD4 exonⅢ48 bp VNTR的6种等位基因和9种基因型,2组最常见的是4次重复等位基因。2组间DRD4基因的基因型及等位基因的频率分布差异有统计学意义(P=0.040,P=0.018)。研究组寻求新奇、伤害避免维度评分高于对照组(P=0.026,P=0.000),合作性、自我定向维度评分低于对照组(均P=0.000)。研究组携带长重复等位基因的患者寻求新奇、伤害避免维度评分高于携带短重复等位基因的患者(P=0.000,P=0.006),合作性维度评分低于携带短重复等位基因的患者(P=0.038)。结论·双相障碍患者攻击行为与DRD4 exonⅢ48 bp VNTR之间可能存在关联。双相障碍攻击行为患者具有冲动、寻求刺激、探索、害怕不确定、报复心强、自私自利等人格特征,携带长重复等位基因患者人格异常更显著。
