【目的】人体中丝裂原激活蛋白激酶14(Mitogen-activated protein kinase 14,MAPK14,又称p38α)主要调控应激和免疫反应。在皮肤组织中,MAPK14信号通路是维护角质形成细胞稳定状态的关键因素,因此需进一步探索MAPK14在皮肤组织中的表达...【目的】人体中丝裂原激活蛋白激酶14(Mitogen-activated protein kinase 14,MAPK14,又称p38α)主要调控应激和免疫反应。在皮肤组织中,MAPK14信号通路是维护角质形成细胞稳定状态的关键因素,因此需进一步探索MAPK14在皮肤组织中的表达状况。【方法】采用互补脱氧核糖核酸末端快速扩增技术和实时荧光定量逆转录聚合酶链式反应技术对该基因的表达进行深度分析。【结果】在来源于婴儿包皮的角质形成细胞中发现了2个MAPK14新型剪接体,即MAPK14-v2a(Genbank注册登记号为OM256527)和MAPK14-v2b(Genbank注册登记号为OM256528)。实时荧光定量逆转录聚合酶链式反应技术的分析结果表明,除皮肤组织外这2个新型剪接体还在其他组织器官中广泛存在,如肾脏、子宫、唾液腺和正常黏膜等。在生长分化期中,发现MAPK14-v2a的表达量逐天显著提高,而MAPK14-v2b的表达则相对稳定;同时还发现,MAPK14-v2a在基底细胞癌患者的病变组织中表达量显著提高。【结论】本研究发现了2种MAPK14新型剪接体,它们是MAPK14在皮肤组织中的表达形式,其中MAPK14-v2a与角质细胞增殖分化呈正相关,并有潜力作为基底细胞癌诊断的分子标记。展开更多
为提升开发商在超高层建筑融资模式选择上的科学性,通过引入案例推理技术(Case Based Reasoning,CBR),在提炼影响其融资模式选择的3层14个指标基础上,运用最近邻策略和网络层次分析的方法构建了该类工程融资模式决策模型。以广州西塔为...为提升开发商在超高层建筑融资模式选择上的科学性,通过引入案例推理技术(Case Based Reasoning,CBR),在提炼影响其融资模式选择的3层14个指标基础上,运用最近邻策略和网络层次分析的方法构建了该类工程融资模式决策模型。以广州西塔为例对模型进行了有效性验证,并以一栋新建的项目为例对决策模型进行了实证研究,提出了针对性的融资方案建议。研究结果表明政府参与和项目开发商的总资产利润率在超高层建筑融资模式选择决策中占主导地位,且该模型在项目融资方案决策上具有可行性和针对性强等优势。展开更多
Objective: To investigate the current status of medical support in military operations other than war (MOOTW) in domestic and overseas. Methods: This paper mainly adopts the descriptive, comparative and induction rese...Objective: To investigate the current status of medical support in military operations other than war (MOOTW) in domestic and overseas. Methods: This paper mainly adopts the descriptive, comparative and induction research methods. The current status of medical support in MOOTW in China, America, Russia, Israel, Canada, had been studied through some official papers, and series of professional books by analysis the precise date in various kinds of emergency event. Results: The contrast shows that there is still a quite huge development in medical support in MOOTW, no matter in China, or in other countries. Conclusion: In order to strengthen the forces, besides building professional rescue teams, it is necessary to take a regular revise of plans, according to missions` needs.展开更多
文摘【目的】人体中丝裂原激活蛋白激酶14(Mitogen-activated protein kinase 14,MAPK14,又称p38α)主要调控应激和免疫反应。在皮肤组织中,MAPK14信号通路是维护角质形成细胞稳定状态的关键因素,因此需进一步探索MAPK14在皮肤组织中的表达状况。【方法】采用互补脱氧核糖核酸末端快速扩增技术和实时荧光定量逆转录聚合酶链式反应技术对该基因的表达进行深度分析。【结果】在来源于婴儿包皮的角质形成细胞中发现了2个MAPK14新型剪接体,即MAPK14-v2a(Genbank注册登记号为OM256527)和MAPK14-v2b(Genbank注册登记号为OM256528)。实时荧光定量逆转录聚合酶链式反应技术的分析结果表明,除皮肤组织外这2个新型剪接体还在其他组织器官中广泛存在,如肾脏、子宫、唾液腺和正常黏膜等。在生长分化期中,发现MAPK14-v2a的表达量逐天显著提高,而MAPK14-v2b的表达则相对稳定;同时还发现,MAPK14-v2a在基底细胞癌患者的病变组织中表达量显著提高。【结论】本研究发现了2种MAPK14新型剪接体,它们是MAPK14在皮肤组织中的表达形式,其中MAPK14-v2a与角质细胞增殖分化呈正相关,并有潜力作为基底细胞癌诊断的分子标记。
文摘为提升开发商在超高层建筑融资模式选择上的科学性,通过引入案例推理技术(Case Based Reasoning,CBR),在提炼影响其融资模式选择的3层14个指标基础上,运用最近邻策略和网络层次分析的方法构建了该类工程融资模式决策模型。以广州西塔为例对模型进行了有效性验证,并以一栋新建的项目为例对决策模型进行了实证研究,提出了针对性的融资方案建议。研究结果表明政府参与和项目开发商的总资产利润率在超高层建筑融资模式选择决策中占主导地位,且该模型在项目融资方案决策上具有可行性和针对性强等优势。
文摘Objective: To investigate the current status of medical support in military operations other than war (MOOTW) in domestic and overseas. Methods: This paper mainly adopts the descriptive, comparative and induction research methods. The current status of medical support in MOOTW in China, America, Russia, Israel, Canada, had been studied through some official papers, and series of professional books by analysis the precise date in various kinds of emergency event. Results: The contrast shows that there is still a quite huge development in medical support in MOOTW, no matter in China, or in other countries. Conclusion: In order to strengthen the forces, besides building professional rescue teams, it is necessary to take a regular revise of plans, according to missions` needs.