OBJECTIVE To study the inhibition of adhesion and invasion of SGC7901 cells into the ECM by integrinβ1 antisense oligodeoxynucleotide asODN.METHODS asODN and control ODN were transfected into SGC7901 cells using lipo...OBJECTIVE To study the inhibition of adhesion and invasion of SGC7901 cells into the ECM by integrinβ1 antisense oligodeoxynucleotide asODN.METHODS asODN and control ODN were transfected into SGC7901 cells using liposomes as vectors. The distribution of the ODN was followed by immunochemistry and changes in the expression of integrinβ1 mRNA and protein were determined by RT-PCR and FCM, respectively. The adhesion and invasion into the ECM were measured by the MTT and Boyden chamber methods, respectively.RESULTS Integrinβ1 asODN which was transfected into SGC7901 cells distributed evenly in the cytoplasm and nucleus. PCR and FCM revealed a weakened band at 489bp and a left-shift curve, respectively. Adhesion and invasion assays showed decreased activity with an inhibition rate of 54% and 76%. The extent of decrease induced by integrinβ1 asODN was larger than that caused by random control ODN (P<0.001).CONCLUSION Transfection of integrinβ1 asODN into SGC7901 cells induced a decrease in the expression of integrinβ1 mRNA and protein,resulting in a decrease in adhesion and invasion into the ECM, with a greater effect than random control ODN.展开更多
The advantage of lidar over other wind sensors is presented in this paper. With more than 20 years research, the development of the space-borne wind lidar is reviewed. Longer-term investigation has made many technolog...The advantage of lidar over other wind sensors is presented in this paper. With more than 20 years research, the development of the space-borne wind lidar is reviewed. Longer-term investigation has made many technologies suitable for the wind lidar measurement from an orbital platform become mature. However, there are still some problems to be solved. In order to obtain the optimal performance in wind detection, great importance is being attached to the simulation of a virtual space-borne wind lidar system on computer as developed by NASA and ESA.展开更多
文摘OBJECTIVE To study the inhibition of adhesion and invasion of SGC7901 cells into the ECM by integrinβ1 antisense oligodeoxynucleotide asODN.METHODS asODN and control ODN were transfected into SGC7901 cells using liposomes as vectors. The distribution of the ODN was followed by immunochemistry and changes in the expression of integrinβ1 mRNA and protein were determined by RT-PCR and FCM, respectively. The adhesion and invasion into the ECM were measured by the MTT and Boyden chamber methods, respectively.RESULTS Integrinβ1 asODN which was transfected into SGC7901 cells distributed evenly in the cytoplasm and nucleus. PCR and FCM revealed a weakened band at 489bp and a left-shift curve, respectively. Adhesion and invasion assays showed decreased activity with an inhibition rate of 54% and 76%. The extent of decrease induced by integrinβ1 asODN was larger than that caused by random control ODN (P<0.001).CONCLUSION Transfection of integrinβ1 asODN into SGC7901 cells induced a decrease in the expression of integrinβ1 mRNA and protein,resulting in a decrease in adhesion and invasion into the ECM, with a greater effect than random control ODN.
基金supported by National High Tech 863 Project(No.2002AA135280)National Natural Science Foundation of China No.40176011International Bureau of BMBF
文摘The advantage of lidar over other wind sensors is presented in this paper. With more than 20 years research, the development of the space-borne wind lidar is reviewed. Longer-term investigation has made many technologies suitable for the wind lidar measurement from an orbital platform become mature. However, there are still some problems to be solved. In order to obtain the optimal performance in wind detection, great importance is being attached to the simulation of a virtual space-borne wind lidar system on computer as developed by NASA and ESA.
