OBJECTIVE： To investigated the role of Mailuoning in the prevention of highglucosemediated cell apoptosis in human umbilical vein endothelial cells （HUVEC） and the mechanisms involved. METHODS： MTT assay was used ...OBJECTIVE： To investigated the role of Mailuoning in the prevention of highglucosemediated cell apoptosis in human umbilical vein endothelial cells （HUVEC） and the mechanisms involved. METHODS： MTT assay was used to investigate cell viability, western blot was used to investigate pro tein expression, and flow cytometric detection technology was used to detect cell apoptosis. RESULTS： Exposure of HUVEC to high glucose （50 mM） significantly suppressed cell viability and increased cell apoptosis compared with normal glu cose （11 mM） （all P〈0.05）. However, Mailuoning pre vented highglucoseinduced HUVEC apoptosis in dosedependent manner. Further studies indicated that Mailuoning suppressed highglucoseinduced p38 mitogenactivated protein kinase phosphoryla tion, but had no effect on extracellular signalregu lated kinase 1/2 and Akt phosphorylation. CONCLUSION： Mailuoning can prevent highglu coseinduced HUVEC apoptosis by suppressing p38 activation.展开更多
基金Supported by Jiangsu Province's Outstanding Leader Prgram of Traditional Chinese Medicine
文摘OBJECTIVE： To investigated the role of Mailuoning in the prevention of highglucosemediated cell apoptosis in human umbilical vein endothelial cells （HUVEC） and the mechanisms involved. METHODS： MTT assay was used to investigate cell viability, western blot was used to investigate pro tein expression, and flow cytometric detection technology was used to detect cell apoptosis. RESULTS： Exposure of HUVEC to high glucose （50 mM） significantly suppressed cell viability and increased cell apoptosis compared with normal glu cose （11 mM） （all P〈0.05）. However, Mailuoning pre vented highglucoseinduced HUVEC apoptosis in dosedependent manner. Further studies indicated that Mailuoning suppressed highglucoseinduced p38 mitogenactivated protein kinase phosphoryla tion, but had no effect on extracellular signalregu lated kinase 1/2 and Akt phosphorylation. CONCLUSION： Mailuoning can prevent highglu coseinduced HUVEC apoptosis by suppressing p38 activation.
