由尖孢镰孢菌苦瓜专化型(Fusarium oxysporum f. sp.momdicae Sun&Huang)引起的苦瓜枯萎病是一种严重影响苦瓜生产的土传维管束病害。本研究利用分割标记的方法构建FoCat1基因的敲除片段,通过PEG介导的原生质体转化方法对FoCat1基...由尖孢镰孢菌苦瓜专化型(Fusarium oxysporum f. sp.momdicae Sun&Huang)引起的苦瓜枯萎病是一种严重影响苦瓜生产的土传维管束病害。本研究利用分割标记的方法构建FoCat1基因的敲除片段,通过PEG介导的原生质体转化方法对FoCat1基因进行敲除,经4对引物验证得到1个阳性转化子ΔFoCat1-3。突变体ΔFoCat1-3在PDA培养基上的菌落大小、菌丝形态、产孢量等生物学特性与野生型相比均无显著性差异,但是其抵抗外源氧胁迫的能力明显减弱,对苦瓜的致病力明显下降。DAB染色结果显示,接种突变体的苦瓜叶片中过氧化氢的含量明显高于接种野生型菌株的苦瓜叶片。研究结果表明,FoCat1可能不参与病菌正常营养生长过程的调控,但是具有清除寄主产生的活性氧,来减弱寄主对病原菌的杀伤作用的功能,从而参与病原菌的致病过程。展开更多
Background Unplanned extubation is associated with adverse outcomes in intensive care unit. The massive burn patient differs from other critically ill patients in many ways. However, little is known about the unplanne...Background Unplanned extubation is associated with adverse outcomes in intensive care unit. The massive burn patient differs from other critically ill patients in many ways. However, little is known about the unplanned decannulation （UD） in Burn Intensive Care Unit. This paper describes the special features of the circumstances and outcome of UD of tracheotomy tube in massive burn patients. Methods A case series study was performed between January 1999 and December 2008 and UD of tracheotomy tube was analyzed retrospectively. A total of 21 patients with 29 UD events were identified. Demographic data, diagnosis, intervention, UD events and outcome of UD patients were collected. Differences in proportions were compared using the chi-square （X2） or Fisher＇s exact test. Results Patients with UD were often burned with head and neck （67%） and combined with inhalation injury （62%）. The majority of them （76%） were transferred patients, occurred early （55%） and were accidental UD （79%）. UD events tended to happen in day shift （90%） and to be associated with the medical procedure that was performing by caregivers at besides （79%）. Loose of the stabilizing rope, medical procedure and tracheotomy malposition were the main causes of UD. Early UD and reintubation failure were associated with patients＇ death. Conclusions UD happened to massive burn patients can lead to patient death. Careful management of respiratory tract was essential for massive burn patients.展开更多
Background More and more Chinese drink hot water from water dispensers while many children were scalded due to this change. The present study aimed to propose a feasible strategy for prevention. Methods A retrospectiv...Background More and more Chinese drink hot water from water dispensers while many children were scalded due to this change. The present study aimed to propose a feasible strategy for prevention. Methods A retrospective study was conducted for all water dispensers related pediatric burns admitted to Changhai Hospital from January 2005 to December 2009. Results The number of new cases and incidences of pediatric burns due to hot water from water dispensers was significantly increasing year after year. In the total 238 involved cases, 175 cases happened on males and 78.9% were at the age of 1-4 years. The burn areas were mainly located in upper extremities. All water dispensers in the surveyed families had no isolate protection devices and 85.2% of their locations were easy for children to reach. Nearly half of the children were in the same room with their guardians when injured. Total 196 burned children were playing the taps of water dispensers before injured, unfortunately, 80.6% of them have not been stopped until burned. Conclusion As the kind of burns is quite serious and with bad outcome, some recommendations should be followed, such as buying water dispensers with protection devices, keeping children from touching them and so on.展开更多
文摘由尖孢镰孢菌苦瓜专化型(Fusarium oxysporum f. sp.momdicae Sun&Huang)引起的苦瓜枯萎病是一种严重影响苦瓜生产的土传维管束病害。本研究利用分割标记的方法构建FoCat1基因的敲除片段,通过PEG介导的原生质体转化方法对FoCat1基因进行敲除,经4对引物验证得到1个阳性转化子ΔFoCat1-3。突变体ΔFoCat1-3在PDA培养基上的菌落大小、菌丝形态、产孢量等生物学特性与野生型相比均无显著性差异,但是其抵抗外源氧胁迫的能力明显减弱,对苦瓜的致病力明显下降。DAB染色结果显示,接种突变体的苦瓜叶片中过氧化氢的含量明显高于接种野生型菌株的苦瓜叶片。研究结果表明,FoCat1可能不参与病菌正常营养生长过程的调控,但是具有清除寄主产生的活性氧,来减弱寄主对病原菌的杀伤作用的功能,从而参与病原菌的致病过程。
文摘Background Unplanned extubation is associated with adverse outcomes in intensive care unit. The massive burn patient differs from other critically ill patients in many ways. However, little is known about the unplanned decannulation （UD） in Burn Intensive Care Unit. This paper describes the special features of the circumstances and outcome of UD of tracheotomy tube in massive burn patients. Methods A case series study was performed between January 1999 and December 2008 and UD of tracheotomy tube was analyzed retrospectively. A total of 21 patients with 29 UD events were identified. Demographic data, diagnosis, intervention, UD events and outcome of UD patients were collected. Differences in proportions were compared using the chi-square （X2） or Fisher＇s exact test. Results Patients with UD were often burned with head and neck （67%） and combined with inhalation injury （62%）. The majority of them （76%） were transferred patients, occurred early （55%） and were accidental UD （79%）. UD events tended to happen in day shift （90%） and to be associated with the medical procedure that was performing by caregivers at besides （79%）. Loose of the stabilizing rope, medical procedure and tracheotomy malposition were the main causes of UD. Early UD and reintubation failure were associated with patients＇ death. Conclusions UD happened to massive burn patients can lead to patient death. Careful management of respiratory tract was essential for massive burn patients.
文摘Background More and more Chinese drink hot water from water dispensers while many children were scalded due to this change. The present study aimed to propose a feasible strategy for prevention. Methods A retrospective study was conducted for all water dispensers related pediatric burns admitted to Changhai Hospital from January 2005 to December 2009. Results The number of new cases and incidences of pediatric burns due to hot water from water dispensers was significantly increasing year after year. In the total 238 involved cases, 175 cases happened on males and 78.9% were at the age of 1-4 years. The burn areas were mainly located in upper extremities. All water dispensers in the surveyed families had no isolate protection devices and 85.2% of their locations were easy for children to reach. Nearly half of the children were in the same room with their guardians when injured. Total 196 burned children were playing the taps of water dispensers before injured, unfortunately, 80.6% of them have not been stopped until burned. Conclusion As the kind of burns is quite serious and with bad outcome, some recommendations should be followed, such as buying water dispensers with protection devices, keeping children from touching them and so on.
