Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics base...Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.展开更多
目的采用新型绿色溶剂天然低共熔溶剂(nature deep eutectic solvents,NADESs)提取玉竹总黄酮,并对其提取工艺、提取效率、溶剂回收利用率进行考察。方法采用响应面法优化玉竹总黄酮提取工艺;通过文献比较评价NADESs提取效率;并结合大...目的采用新型绿色溶剂天然低共熔溶剂(nature deep eutectic solvents,NADESs)提取玉竹总黄酮,并对其提取工艺、提取效率、溶剂回收利用率进行考察。方法采用响应面法优化玉竹总黄酮提取工艺;通过文献比较评价NADESs提取效率;并结合大孔树脂吸附法回收NADESs。结果由醋酸钠和乳酸合成的NADESs(物质的量比为1∶5)具有最高的提取率,经响应面法优化后,在水比例(水体积与溶液体积比)22%,提取温度51℃,提取时间21 min和液固比21 m L/g下,总黄酮提取率可达20.13 mg/g;而且,NADESs提取率显著高于文献报道的已有方法;回收实验表明,NAK-9对总黄酮的吸附效率(88.67%)显著高于D-101(77.33%)和AB-8(79.15%)。以甲醇为溶剂,其解吸附率达80.46%。回收后的NADESs重复使用的提取率达18.79 mg/g,回收利用率为94.56%。结论 NADESs作为提取介质具有高效、绿色环保及可重复利用等优点,可用于玉竹总黄酮类化合物的提取,同时为NADESs作为提取介质应用于其他中药有效组分的提取提供了新思路。展开更多
基金We thank for the funding support from the National Natural Science Foundation of China(No.81503041)Natural Science Foundation of Hunan Province(No.2017JJ4045)Changsha Science and Technology Project(No.kq1701073).
文摘Objective Pelvic inflammatory disease(PID)is one of the most common gynaecological diseases.Here,this thesis aims to investigate the therapeutic effects of Prunella vulgaris L.oil on the PID by using metabolomics based on gas chromatographymass spectrometry(GC-MS)to address this challenge.Methods First,measurements of pro-inflammatory cytokines and histological analysis of the uterus were conducted to validate the successful generation of a PID rat model.Furthermore,the volatile oil from Prunella vulgaris L.was administered to treat PID rats.Serum samples were collected before and after treatment and analyzed by GC-MS to generate metabolite profiles for each sample.The information generated from the qualitative and quantitative analysis of these metabolites was applied to distinguish between the PID model and normal control groups.Results Some metabolites,such as acetic acid,succinic acid,glyceric acid,(R*,S*)-3,4-dihydroxybutanoic acid,3-hydroxyphenylacetic acid,D-ribose and myo-inositol showed a higher contribution in the classification model;thus,they can be considered as potential biomarkers.Furthermore,the therapeutic effect of the volatile oil extracted from Prunella vulgaris L.could also be visualized using GC-MS-based metabolomics.Conclusions The results show that metabolomics studies are invaluable for disease diagnosis and therapeutic effect estimation.
文摘目的采用新型绿色溶剂天然低共熔溶剂(nature deep eutectic solvents,NADESs)提取玉竹总黄酮,并对其提取工艺、提取效率、溶剂回收利用率进行考察。方法采用响应面法优化玉竹总黄酮提取工艺;通过文献比较评价NADESs提取效率;并结合大孔树脂吸附法回收NADESs。结果由醋酸钠和乳酸合成的NADESs(物质的量比为1∶5)具有最高的提取率,经响应面法优化后,在水比例(水体积与溶液体积比)22%,提取温度51℃,提取时间21 min和液固比21 m L/g下,总黄酮提取率可达20.13 mg/g;而且,NADESs提取率显著高于文献报道的已有方法;回收实验表明,NAK-9对总黄酮的吸附效率(88.67%)显著高于D-101(77.33%)和AB-8(79.15%)。以甲醇为溶剂,其解吸附率达80.46%。回收后的NADESs重复使用的提取率达18.79 mg/g,回收利用率为94.56%。结论 NADESs作为提取介质具有高效、绿色环保及可重复利用等优点,可用于玉竹总黄酮类化合物的提取,同时为NADESs作为提取介质应用于其他中药有效组分的提取提供了新思路。
