Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis a...Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.展开更多
基金supported by the Natural Science Foundation of China(31271760)the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops,Ministry of Agriculture,China
文摘Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.
