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稻米淀粉体外消化试验方法的优化及应用
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作者 李颖颖 蒋彦婕 +10 位作者 李文奇 陶亚军 王芳权 陈智慧 许扬 王军 范方军 朱建平 夏士健 李霞 杨杰 《江苏农业学报》 CSCD 北大核心 2023年第2期539-546,共8页
为实现快捷、经济的稻米升糖指数(GI)估测,首先以不同直链淀粉含量的水稻品种紫金糯2号、南粳04062、宁西优Y16稻米为研究材料,分析了样品前处理方式、α-淀粉酶和淀粉糖苷酶的添加量及方式等对稻米淀粉水解的影响,明确了稻米淀粉体外... 为实现快捷、经济的稻米升糖指数(GI)估测,首先以不同直链淀粉含量的水稻品种紫金糯2号、南粳04062、宁西优Y16稻米为研究材料,分析了样品前处理方式、α-淀粉酶和淀粉糖苷酶的添加量及方式等对稻米淀粉水解的影响,明确了稻米淀粉体外消化的适宜前处理方式、α-淀粉酶和淀粉糖苷酶的适宜添加量和方式,进而根据淀粉水解指数和水解率进行了稻米升糖指数的估算。结果表明,稻米升糖指数估算适宜的淀粉体外消化试验方法是0.75 g精米加水煮熟后,人工研棒挤压30下,加pH6.8的磷酸盐缓冲液定容至15 ml,同时加入300 U/ml的α-淀粉酶和50 U/ml的淀粉糖苷酶各1 ml联合水解60 min,通过监测水解液葡萄糖含量动态变化,计算1 h内的淀粉水解指数及1 h的水解率,即可实现稻米升糖指数的估算;9个稻米样本的GI估算结果表明本研究建立的试验方法能实现低GI稻米资源和遗传材料的初步筛选。 展开更多
关键词 水稻 淀粉水解率 升糖指数(GI) 淀粉体外消化
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水稻光温敏核不育基因tms5与pms3的互作效应 被引量:3
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作者 王芳权 范方军 +10 位作者 夏士健 宗寿余 郑天清 王军 李文奇 许扬 陈智慧 蒋彦婕 陶亚军 仲维功 杨杰 《作物学报》 CAS CSCD 北大核心 2020年第3期317-329,共13页
tms5与pms3是水稻的光温敏核不育基因,其功能位点已经明确,然而它们在两系不育系中的效应尚不清楚。本研究针对tms5与pms3基因功能位点,分别设计了功能标记AS-TMS5和CAPS-PMS3。经鉴定发现,这2个功能标记能准确区分不育、可育性状对应... tms5与pms3是水稻的光温敏核不育基因,其功能位点已经明确,然而它们在两系不育系中的效应尚不清楚。本研究针对tms5与pms3基因功能位点,分别设计了功能标记AS-TMS5和CAPS-PMS3。经鉴定发现,这2个功能标记能准确区分不育、可育性状对应的隐性纯合、杂合和显性纯合3种基因型。利用AS-TMS5和CAPS-PMS3对培矮64S/9311、广占63S/湘恢47和粤光S/宁恢108的F2群体单株的基因型及育性的关系分析发现,tms5基因是广占63S和粤光S控制光温敏不育性状的主效基因,而pms3基因在培矮64S和粤光S中并不能独立起作用,还需要与其他基因共同调控。进一步分析粤光S/宁恢108的F2:3群体基因型与育性的关系,发现在粤光S/宁恢108背景下,携带pms3基因的株系几乎都表现可育,而携带tms5基因的株系在较高气温条件下表现不育,但育性转换温度可能较高;而携带tms5与pms3基因的株系育性转换温度比仅携带tms5基因的株系低,这为聚合2个基因选育不育性状稳定的光温敏不育系提供了思路和方法。 展开更多
关键词 tms5 pms3 功能标记 光敏不育 温敏不育
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Plant Temperature and Its Simulation Model of Thermo-Sensitive Genic Male Sterile Rice 被引量:4
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作者 LU Chuan-gen xia shi-jian +2 位作者 CHEN Jing Hu Ning YAO Ke-min 《Rice science》 SCIE 2008年第3期223-231,共9页
Plant temperature (Tp) and its relations to the microclimate of rice colony and irrigation water were studied using a thermo-sensitive genic male sterile (TGMS) rice line, Pei'ai 64S. Significant differences in t... Plant temperature (Tp) and its relations to the microclimate of rice colony and irrigation water were studied using a thermo-sensitive genic male sterile (TGMS) rice line, Pei'ai 64S. Significant differences in the daily change of temperature were detected between Tp and air temperature at the height of 150 cm (TA). From 8:00 to 20:00, Tp was lower than TA, but they were similar during 21:00 to next 7:00. The maximum Tp occurred one hour earlier than the maximum TA, though they both reached the minimum at 6:00. Tp fluctuated less than TA. At the same height, during 6:00-13:00, Tp was higher than air temperature (Ta), and Tp reached the maximum one hour earlier than Ta. During the rest time on sunny day, Tp was close to or even a little lower than Ta. On overcast day, Tp was higher than Ta in the whole day, and both maximized at the same time. In addition, Tp was regulated by solar radiation, cloudage and wind speed in daytime, and by irrigation water at night. The present study indicated that a TA of 29.6℃ was the critical point, at which Tp was increased or decreased by irrigation water. Tp and the difference between water and air temperatures showed a conic relation. Tp fluctuation was also regulated by the absorption or reflection of solar radiation by leaves during daytime and release of heat energy during nighttime. By analysis on correlation and regression simulation, two models of Tp were established. 展开更多
关键词 RICE plant temperature air temperature simulation model thermo-sensitive genic male sterility
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Plant Type and Its Effects on Canopy Structure at Heading Stage in Various Ecological Areas for a Two-line Hybrid Rice Combination,Liangyoupeijiu 被引量:3
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作者 LU Chuan-gen Hu Ning +2 位作者 YAO Ke-min xia shi-jian QI Qing-ming 《Rice science》 SCIE 2010年第3期235-242,共8页
A two-line hybrid rice combination, Liangyoupeijiu, was used to estimate several factors of plant type, and environmental models for these factors at the heading stage were established using the data of eight ecologic... A two-line hybrid rice combination, Liangyoupeijiu, was used to estimate several factors of plant type, and environmental models for these factors at the heading stage were established using the data of eight ecological experimental sites in 2006 and 2007. According to climatic data from 1951 to 2005, the differences in those factors and their effects on plant canopy were analyzed for four rice cropping areas in China, including South China, the middle-lower reaches of the Yangtze River, Sichuan Basin, and river valley in Yunnan, China. The thickness of leaf layer (the distance from pulvinus of the third leaf from the top to the tip of flag leaf) and distribution of leaf area could be used as candidate indices for the plant type of a rice canopy. 展开更多
关键词 hybrid rice plant type MODEL ecological area canopy structure INDEX
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Research on Theories and Techniques of Irrigation for Safeguarding Seed Production of Two-Line Hybrid Rice 被引量:3
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作者 ZOU Jiang-shi LUE Chuan-gen +2 位作者 YAO Ke-min HU Ning xia shi-jian 《Agricultural Sciences in China》 CAS CSCD 2006年第8期596-602,共7页
By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defi... By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei'ai64S, were measured. Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1 ℃. For the present study, the following irrigation techniques were put forth: the water depth of 15-20 cm, current water used, irrigating after 17:00 and bailing at 10:00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice. 展开更多
关键词 two-line hybrid rice seed production PURITY IRRIGATION
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