目的利用CRISPR/Cas9技术构建牛磺酸转运体基因(solute carrier family 6 member 6,Slc6a6)敲除大鼠,繁殖并鉴定,为研究牛磺酸缺失对神经系统疾病的影响提供稳定的大鼠模型。方法针对Slc6a6基因第5外显子,设计向导RNA(single-guide RNA,...目的利用CRISPR/Cas9技术构建牛磺酸转运体基因(solute carrier family 6 member 6,Slc6a6)敲除大鼠,繁殖并鉴定,为研究牛磺酸缺失对神经系统疾病的影响提供稳定的大鼠模型。方法针对Slc6a6基因第5外显子,设计向导RNA(single-guide RNA,sgRNA)介导Cas9核酸酶与靶点DNA特异性结合,并切割基因组DNA,被切割后的DNA进行重组修复,从而实现基因的敲除。通过基因型鉴定和测序分析检测新生大鼠基因型。利用Real-time PCR、Western blot技术和免疫组化等方法,分析大鼠脑组织的牛磺酸转运体(taurine transporter,TauT)的mRNA表达和蛋白表达,建立Slc6a6基因敲除大鼠模型。结果 F3代出现21只Slc6a6基因敲除纯合子(TauT^(-/-)),54只杂合子(TauT^(+/-)),27只阴性(TauT^(+/+)),F3代纯合率约为20.59%,基本符合孟德尔遗传定律。Slc6a6基因敲除纯合子大鼠脑组织mRNA水平基本不表达,TauT蛋白表达水平显著低于同窝阴性大鼠。结论本研究利用CRISPR/Cas9系统定向敲除Slc6a6基因,成功构建Slc6a6基因敲除大鼠模型。展开更多
目的调查伊犁地区婴幼儿发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的院内筛查结果,并分析影响DDH发生的风险因素。方法按照整群抽样法,选取2019年12月至2022年6月于新疆生产建设兵团第四师医院儿保门诊及骨科门诊...目的调查伊犁地区婴幼儿发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的院内筛查结果,并分析影响DDH发生的风险因素。方法按照整群抽样法,选取2019年12月至2022年6月于新疆生产建设兵团第四师医院儿保门诊及骨科门诊进行DDH普查的5536例婴幼儿为研究对象。筛选出符合DDH诊断标准的患儿为观察组(n=35),另选择100例正常婴幼儿为对照组。采用单因素和多因素分析法确立影响婴幼儿DDH发生的独立风险因素。结果初筛阳性者39例,复筛阳性者35例,阳性率6.32‰。单因素分析结果显示,观察组女患儿、二胎及以上、剖腹产、产时臀位、有家族史、高海拔地区、居住环境室温<18℃、襁褓时绑腿的比例高于对照组(P<0.05)。logistic回归分析显示:生产方式、地域、居住环境室温及襁褓方式是影响婴幼儿发生DDH的独立风险因素(P<0.05)。结论剖腹产、高海拔地区、居住环境室温<18℃及襁褓中绑腿是婴幼儿DDH的发生相关,可为临床筛查和诊治提供一定的参考依据。展开更多
A multiresolution hexahedron element is presented with a new multiresolution analysis(MRA)framework.The MRA framework is formulated out of a mutually nesting displacement subspace sequence,whose basis functions are co...A multiresolution hexahedron element is presented with a new multiresolution analysis(MRA)framework.The MRA framework is formulated out of a mutually nesting displacement subspace sequence,whose basis functions are constructed of scaling and shifting on element domain of a basic node shape function.The basic node shape function is constructed from shifting to other seven quadrants around a specific node of a basic isoparametric element in one quadrant and joining the corresponding node shape functions of eight elements at the specific node.The MRA endows the proposed element with the resolution level(RL)to adjust structural analysis accuracy.As a result,the traditional 8-node hexahedron element is a monoresolution one and also a special case of the proposed element.The meshing for the monoresolution finite element model is based on the empiricism while the RL adjusting for the multiresolution is laid on the solid mathematical basis.The simplicity and clarity of shape function construction with the Kronecker delta property and the rational MRA enable the proposed element method to be more rational,easier and efficient in its implementation than the conventional mono-resolution solid element method or other MRA methods.The multiresolution hexahedron element method is more adapted to dealing with the accurate computation of structural problems.展开更多
文摘目的调查伊犁地区婴幼儿发育性髋关节发育不良(developmental dysplasia of the hip,DDH)的院内筛查结果,并分析影响DDH发生的风险因素。方法按照整群抽样法,选取2019年12月至2022年6月于新疆生产建设兵团第四师医院儿保门诊及骨科门诊进行DDH普查的5536例婴幼儿为研究对象。筛选出符合DDH诊断标准的患儿为观察组(n=35),另选择100例正常婴幼儿为对照组。采用单因素和多因素分析法确立影响婴幼儿DDH发生的独立风险因素。结果初筛阳性者39例,复筛阳性者35例,阳性率6.32‰。单因素分析结果显示,观察组女患儿、二胎及以上、剖腹产、产时臀位、有家族史、高海拔地区、居住环境室温<18℃、襁褓时绑腿的比例高于对照组(P<0.05)。logistic回归分析显示:生产方式、地域、居住环境室温及襁褓方式是影响婴幼儿发生DDH的独立风险因素(P<0.05)。结论剖腹产、高海拔地区、居住环境室温<18℃及襁褓中绑腿是婴幼儿DDH的发生相关,可为临床筛查和诊治提供一定的参考依据。
基金supported by the Foundation of Municipal Key Laboratory of Geomechanics and Geological Environment Protection at Chongqing Institute of Logistics Engineering of PLA(Grant No.GKLGGP 2013-02)the National Natural Science Foundation of China(Grant No.51178222)
文摘A multiresolution hexahedron element is presented with a new multiresolution analysis(MRA)framework.The MRA framework is formulated out of a mutually nesting displacement subspace sequence,whose basis functions are constructed of scaling and shifting on element domain of a basic node shape function.The basic node shape function is constructed from shifting to other seven quadrants around a specific node of a basic isoparametric element in one quadrant and joining the corresponding node shape functions of eight elements at the specific node.The MRA endows the proposed element with the resolution level(RL)to adjust structural analysis accuracy.As a result,the traditional 8-node hexahedron element is a monoresolution one and also a special case of the proposed element.The meshing for the monoresolution finite element model is based on the empiricism while the RL adjusting for the multiresolution is laid on the solid mathematical basis.The simplicity and clarity of shape function construction with the Kronecker delta property and the rational MRA enable the proposed element method to be more rational,easier and efficient in its implementation than the conventional mono-resolution solid element method or other MRA methods.The multiresolution hexahedron element method is more adapted to dealing with the accurate computation of structural problems.
