Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtub...Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule(MT).However,there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton.Therefore,the identification and portrayal of G.hirsutum TPX2 genes can provide key targets for molecular manipula-tion in the breeding of cotton fiber improvement.Result In this study,TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMP-ENED2-LIKE(WDLs)and quite conservative in quantity.GhWDL3 was significantly up-regulated in 15 days post anthe-sis fibers of ZRI-015(an upland cotton with longer and stronger fiber).GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis,which may indirectly regulate cotton fiber cell morphology during fiber development.Virus induced gene silencing(VIGS)results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes.Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.展开更多
【目的】为了分析香花油茶叶绿体基因组密码子偏好性,以筛选到的53条基因编码序列为研究对象,进行密码子组成成分和偏好性分析,进而找出香花油茶叶绿体基因组最优密码子,为该物种基因组研究及其遗传改良提供理论基础。【方法】利用Codon...【目的】为了分析香花油茶叶绿体基因组密码子偏好性,以筛选到的53条基因编码序列为研究对象,进行密码子组成成分和偏好性分析,进而找出香花油茶叶绿体基因组最优密码子,为该物种基因组研究及其遗传改良提供理论基础。【方法】利用Codon W 1.4.2、在线软件CUSP及Python3.7对香花油茶叶绿体基因组序列进行中性绘图、ENC-plot和PR2-plot绘图分析,探究影响密码子偏好性的主要因素,并通过ΔRSCU法预测最优密码子。【结果】1)香花油茶叶绿体基因组中,RSCU>1的密码子数目为31个,其中以U结尾的有16个,以A结尾的有12个,以G结尾的有3个;2)密码子三碱基中的平均GC_all含量为37.85%,且GC1(45.87%)>GC2(39.67)>GC3(28.03%);3)ENC-plot分析发现,ENC值位于24.1~61.0之间,平均值为40.09,香花油茶的ENC范围明显大于普通油茶(35.23~56.67);4)相关性分析发现,GC12与GC3相关性不显著,此外密码子第三个碱基上U的使用频率高于A,G的使用频率高于C;5)通过ΔRSCU值法,找出香花油茶叶绿体基因组中5个最优密码子,分别为GAU、GGU、UUG、CCU、AGA。【结论】香花油茶叶绿体基因组密码子碱基多以A或U结尾。该物种叶绿体基因组中密码子偏好性不仅受碱基突变影响,自然选择也发挥着不可替代的作用。鉴定出5个最优密码子,存在较少最优密码子的原因可能是由于该物种在进化过程中受到纯化选择的压力。展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200300)China Agriculture Research System(CARS-15-01).
文摘Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule(MT).However,there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton.Therefore,the identification and portrayal of G.hirsutum TPX2 genes can provide key targets for molecular manipula-tion in the breeding of cotton fiber improvement.Result In this study,TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMP-ENED2-LIKE(WDLs)and quite conservative in quantity.GhWDL3 was significantly up-regulated in 15 days post anthe-sis fibers of ZRI-015(an upland cotton with longer and stronger fiber).GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis,which may indirectly regulate cotton fiber cell morphology during fiber development.Virus induced gene silencing(VIGS)results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes.Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.
文摘【目的】为了分析香花油茶叶绿体基因组密码子偏好性,以筛选到的53条基因编码序列为研究对象,进行密码子组成成分和偏好性分析,进而找出香花油茶叶绿体基因组最优密码子,为该物种基因组研究及其遗传改良提供理论基础。【方法】利用Codon W 1.4.2、在线软件CUSP及Python3.7对香花油茶叶绿体基因组序列进行中性绘图、ENC-plot和PR2-plot绘图分析,探究影响密码子偏好性的主要因素,并通过ΔRSCU法预测最优密码子。【结果】1)香花油茶叶绿体基因组中,RSCU>1的密码子数目为31个,其中以U结尾的有16个,以A结尾的有12个,以G结尾的有3个;2)密码子三碱基中的平均GC_all含量为37.85%,且GC1(45.87%)>GC2(39.67)>GC3(28.03%);3)ENC-plot分析发现,ENC值位于24.1~61.0之间,平均值为40.09,香花油茶的ENC范围明显大于普通油茶(35.23~56.67);4)相关性分析发现,GC12与GC3相关性不显著,此外密码子第三个碱基上U的使用频率高于A,G的使用频率高于C;5)通过ΔRSCU值法,找出香花油茶叶绿体基因组中5个最优密码子,分别为GAU、GGU、UUG、CCU、AGA。【结论】香花油茶叶绿体基因组密码子碱基多以A或U结尾。该物种叶绿体基因组中密码子偏好性不仅受碱基突变影响,自然选择也发挥着不可替代的作用。鉴定出5个最优密码子,存在较少最优密码子的原因可能是由于该物种在进化过程中受到纯化选择的压力。
