The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of s...The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.展开更多
This study examines the reflection of regular and irregular waves from a partially perforated caisson breakwater located on a step bed. The step bed is treated as an idealized rubble mound foundation. Based on the lin...This study examines the reflection of regular and irregular waves from a partially perforated caisson breakwater located on a step bed. The step bed is treated as an idealized rubble mound foundation. Based on the linear potential theory, an analytical solution is developed to calculate the reflection coefficient of the structure subjected to regular waves. The matched eigenfunction expansion method is used for the solution. The regular wave method is also extended to irregular waves using a linear transfer function. The calculated results obtained for limiting cases are exactly the same as corresponding results given by the previous researchers. The present predictions also agree well with experimental data in the published literatures. Numerical experiments are conducted to examine the variations of the reflection coefficient versus its main effect factors, and some interesting results are presented.展开更多
The physical simulation method of wave groups in a wave flume is proposed and verified by the exper- iments. The experimental results demonstrate that random waves with desired wave groupiness, which simultaneously in...The physical simulation method of wave groups in a wave flume is proposed and verified by the exper- iments. The experimental results demonstrate that random waves with desired wave groupiness, which simultaneously includes the wave group height and length, can be generated satisfactorily at the specified position in a wave flume using the proposed method. Furthermore, the transformation properties of the wave groupiness along the fiat-bottomed wave flume are investigated based on the physically simulated waves. Associated proposals with the physical simulation of wave groups are given.展开更多
Continuously increasing applications of robot technologies in unstructured environments put higher requirements on the robotic grippers'performance,such as interaction capability,output force range,and controllabi...Continuously increasing applications of robot technologies in unstructured environments put higher requirements on the robotic grippers'performance,such as interaction capability,output force range,and controllability.However,currently,it is hard for either rigid or soft grippers to meet these requirements,as single soft or rigid structures alone are difficult to effectively overcome/alleviate their inherent defects,e.g.,low compliance of rigid structures and low output force of soft structures.To deal with these difficulties,soft-rigid coupling grippers,or hybrid grippers are proposed.Technically,the soft-rigid coupling is a promising design that combines soft and rigid structures,in order to exploit their respective advantages,such as the strength of rigid structures and compliance of soft structures,in the same set of the gripper system.For the first time,herein,this paper systematically discusses the collaboration strategies of the existing hybrid robotic grippers,by classifying them as Rigid-activesoft-passive,Rigid-passive-soft-active,and Rigid-active-soft-active.At the same time,we introduce the integrated fabrication methods of hybrid grippers,through which the soft and rigid structures with great stiffness and property differences can be coupled together to construct a stable system.Also,possible performance improvements on soft-rigid coupling design for gripper systems are discussed.展开更多
文摘The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.
基金The Natural Science Foundation of Shandong Province under contract No Q2008F01the Specialized Research Fund for the Doctoral Program of Higher Education under contract No 200804231006the National Natural Science Foundation of China under contract Nos 40876047 and 50609001
文摘This study examines the reflection of regular and irregular waves from a partially perforated caisson breakwater located on a step bed. The step bed is treated as an idealized rubble mound foundation. Based on the linear potential theory, an analytical solution is developed to calculate the reflection coefficient of the structure subjected to regular waves. The matched eigenfunction expansion method is used for the solution. The regular wave method is also extended to irregular waves using a linear transfer function. The calculated results obtained for limiting cases are exactly the same as corresponding results given by the previous researchers. The present predictions also agree well with experimental data in the published literatures. Numerical experiments are conducted to examine the variations of the reflection coefficient versus its main effect factors, and some interesting results are presented.
基金The National High Technology Research and Development Program of China under contract No.2012AA052602the National Natural Science Foundation of China under contract Nos 51079023 and 50921001
文摘The physical simulation method of wave groups in a wave flume is proposed and verified by the exper- iments. The experimental results demonstrate that random waves with desired wave groupiness, which simultaneously includes the wave group height and length, can be generated satisfactorily at the specified position in a wave flume using the proposed method. Furthermore, the transformation properties of the wave groupiness along the fiat-bottomed wave flume are investigated based on the physically simulated waves. Associated proposals with the physical simulation of wave groups are given.
基金supported by the National Natural Science Foundation of China(Grant Nos.52188102 and U1613204)。
文摘Continuously increasing applications of robot technologies in unstructured environments put higher requirements on the robotic grippers'performance,such as interaction capability,output force range,and controllability.However,currently,it is hard for either rigid or soft grippers to meet these requirements,as single soft or rigid structures alone are difficult to effectively overcome/alleviate their inherent defects,e.g.,low compliance of rigid structures and low output force of soft structures.To deal with these difficulties,soft-rigid coupling grippers,or hybrid grippers are proposed.Technically,the soft-rigid coupling is a promising design that combines soft and rigid structures,in order to exploit their respective advantages,such as the strength of rigid structures and compliance of soft structures,in the same set of the gripper system.For the first time,herein,this paper systematically discusses the collaboration strategies of the existing hybrid robotic grippers,by classifying them as Rigid-activesoft-passive,Rigid-passive-soft-active,and Rigid-active-soft-active.At the same time,we introduce the integrated fabrication methods of hybrid grippers,through which the soft and rigid structures with great stiffness and property differences can be coupled together to construct a stable system.Also,possible performance improvements on soft-rigid coupling design for gripper systems are discussed.
