Growth and energy budget of the polychaete,Neanthes japonica,at various temperatures(17,20,23,26 and 29℃) were investigated in this study. The growth,as indicated by final dry weight and specific growth rate(SGR),inc...Growth and energy budget of the polychaete,Neanthes japonica,at various temperatures(17,20,23,26 and 29℃) were investigated in this study. The growth,as indicated by final dry weight and specific growth rate(SGR),increased with increasing temperature,with the maximum level at 26℃,and then decreased significantly at 29℃. A similar trend was observed in feeding rate,food conversion efficiency(FCE) and apparent digestive rate(ADR). However,no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation,faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expenditure terms(respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food energy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition,regression equations describing the relationship between feeding rate,faecal production,SGR,FCE and temperature were obtained. The optimum temperatures for feeding rate,FCE and SGR were estimated at 25.01℃,24.24℃ and 24.73 ℃,respectively,from the regression equations.展开更多
The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement an...The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement and/or mood disorders.To understand cellular complexity,differential gene expression and transcriptional regulatory network of human basal ganglia under normal condition,we performed single cell RNA sequencing of 2424 cells sampled from the basal ganglia of human postmortem brains including caudate,putamen,substantia nigra and globus pallidus.展开更多
With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulatin...With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulating donorbrains very fast(estimately 80 brains till now)from people with normal aging or various neurodegenerative diseases.The basal ganglia is critical for movement control,behavior,cognition,emotion and reward.Many neurodegenerative diseases are related to the abnormality or aging of human basal ganglia.Single nuclei transcriptomic profiles of both normal and diseased human basal ganglia are still sparse.In our study,we systematically examined different nuclei(caudate,putamen,globus pallidus,and substantia nigra)in human basal ganglia from 6 control and 5 diseased donors(2 psychiatric diseases,2 PD and 1 AD)using combinatory indexing single nuclei RNA sequencing.Gene expression was measured on a total of 22309 control and 42590 diseased single cells.展开更多
基金supported by the National Natural Sci-ence Foundation of China (No. 30490233).
文摘Growth and energy budget of the polychaete,Neanthes japonica,at various temperatures(17,20,23,26 and 29℃) were investigated in this study. The growth,as indicated by final dry weight and specific growth rate(SGR),increased with increasing temperature,with the maximum level at 26℃,and then decreased significantly at 29℃. A similar trend was observed in feeding rate,food conversion efficiency(FCE) and apparent digestive rate(ADR). However,no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation,faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expenditure terms(respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food energy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition,regression equations describing the relationship between feeding rate,faecal production,SGR,FCE and temperature were obtained. The optimum temperatures for feeding rate,FCE and SGR were estimated at 25.01℃,24.24℃ and 24.73 ℃,respectively,from the regression equations.
文摘The basal ganglia is a critical subcortical nuclei in several parallel and closed circuits,which mainly modulate thalamus-cortex activity.Dysfunction in any one of these loops connecting cortex can lead to movement and/or mood disorders.To understand cellular complexity,differential gene expression and transcriptional regulatory network of human basal ganglia under normal condition,we performed single cell RNA sequencing of 2424 cells sampled from the basal ganglia of human postmortem brains including caudate,putamen,substantia nigra and globus pallidus.
文摘With the advent of single cell technology and its rapid applications in the biomedical field,it becomes possible to identify,categorize and characterize each single cell in the brain.Shanghai Brain Bank is accumulating donorbrains very fast(estimately 80 brains till now)from people with normal aging or various neurodegenerative diseases.The basal ganglia is critical for movement control,behavior,cognition,emotion and reward.Many neurodegenerative diseases are related to the abnormality or aging of human basal ganglia.Single nuclei transcriptomic profiles of both normal and diseased human basal ganglia are still sparse.In our study,we systematically examined different nuclei(caudate,putamen,globus pallidus,and substantia nigra)in human basal ganglia from 6 control and 5 diseased donors(2 psychiatric diseases,2 PD and 1 AD)using combinatory indexing single nuclei RNA sequencing.Gene expression was measured on a total of 22309 control and 42590 diseased single cells.
