By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from...By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from the skin of hybrid sturgeon with two extraction methods. The yields of ASC and PSC based on the wet weight of skin were 5.73 ± 0.11% and 10.26 ± 0.39%, respectively. The denaturation and melting points of ASC(26.83 ℃ and 110.49 ℃) and PSC(26.54 ℃ and 102.99 ℃) were assessed by Circular dichroism(CD) and Differential scanning calorimetry(DSC). ASC and PSC appeared to be dense sheet-like film linked by random-coiled filaments under scanning electron microscopy(SEM). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Fourier transform infrared spectroscopy(FTIR) confirmed that both the ASC and PSC were Type I collagen and maintained a complete triple helix structure. These results indicated that both ASC and PSC possessed good biological activity and could be widely used in medical biomaterials and other fields.展开更多
Disturbance by rodents alters the morphologies and nutrients of plants as well as the physical-chemical properties of the soils.Changes in plants are considered to be mechanisms of defense against the disturbance by r...Disturbance by rodents alters the morphologies and nutrients of plants as well as the physical-chemical properties of the soils.Changes in plants are considered to be mechanisms of defense against the disturbance by rodents.Rodents gnaw on the assimilating branches of Haloxylon ammodendron(CA Mey.)Bunge and burrow under the bushes in the desert ecosystems of Xinjiang,China.However,eco-physiological responses of different age groups of H.ammodendron to the disturbance by rodents are not well understood.In this study,soil physical-chemical properties under the shrubs and the above-ground morphological,physiological and biochemical features of assimilating branches of H.ammodendron of different age groups(i.e.,young,30−100 cm;middle-aged,100−200 cm;and mature,>200 cm)in burrowed and non-burrowed(control)areas were studied in 2018.We found that disturbance by rodents significantly increased the crown width and total branching rates of young and middle-aged H.ammodendron.Photosynthetic pigment contents of assimilating branches of H.ammodendron were significantly reduced under the disturbance by rodents.In term of plant nutrients,the main differences among different age groups of H.ammodendron under the disturbance by rodents occurred in the total soluble sugar and reducing sugar contents that decreased in young plants,increased in middle-aged plants,and did not affect in mature plants.Crude protein and phosphorus contents significantly increased,while crude fiber and calcium contents significantly decreased in young plants.Crude fat and calcium contents significantly decreased in middle-aged plants.Soil organic matter(SOM),total nitrogen(TN),available nitrogen(AN)and available potassium(AK)contents in the topsoil(0–20 cm),which are conducive to forming''fertile islands'',also increased under the disturbance by rodents.In particular,soil AN and AK were the major factors affecting the above-ground morphological characteristics of H.ammodendron in burrowed areas.Overall,the response and defense strategies of H.ammodendron to the disturbance by rodents differed among different age groups,and the effect of the disturbance by rodents on H.ammodendron gradually weakened with the increasing plant age.展开更多
基金Funded by the National Natural Science Foundation of China(No.51173143)
文摘By using the wastes fish skin of sturgeon processed as a raw material, a macromolecule biomaterial of collagen was extracted. Acid-soluble collagen(ASC) and pepsin-soluble collagen(PSC) were successfully isolated from the skin of hybrid sturgeon with two extraction methods. The yields of ASC and PSC based on the wet weight of skin were 5.73 ± 0.11% and 10.26 ± 0.39%, respectively. The denaturation and melting points of ASC(26.83 ℃ and 110.49 ℃) and PSC(26.54 ℃ and 102.99 ℃) were assessed by Circular dichroism(CD) and Differential scanning calorimetry(DSC). ASC and PSC appeared to be dense sheet-like film linked by random-coiled filaments under scanning electron microscopy(SEM). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Fourier transform infrared spectroscopy(FTIR) confirmed that both the ASC and PSC were Type I collagen and maintained a complete triple helix structure. These results indicated that both ASC and PSC possessed good biological activity and could be widely used in medical biomaterials and other fields.
基金The study was funded by the National Natural Science Foundation of China(41561010,31560177)。
文摘Disturbance by rodents alters the morphologies and nutrients of plants as well as the physical-chemical properties of the soils.Changes in plants are considered to be mechanisms of defense against the disturbance by rodents.Rodents gnaw on the assimilating branches of Haloxylon ammodendron(CA Mey.)Bunge and burrow under the bushes in the desert ecosystems of Xinjiang,China.However,eco-physiological responses of different age groups of H.ammodendron to the disturbance by rodents are not well understood.In this study,soil physical-chemical properties under the shrubs and the above-ground morphological,physiological and biochemical features of assimilating branches of H.ammodendron of different age groups(i.e.,young,30−100 cm;middle-aged,100−200 cm;and mature,>200 cm)in burrowed and non-burrowed(control)areas were studied in 2018.We found that disturbance by rodents significantly increased the crown width and total branching rates of young and middle-aged H.ammodendron.Photosynthetic pigment contents of assimilating branches of H.ammodendron were significantly reduced under the disturbance by rodents.In term of plant nutrients,the main differences among different age groups of H.ammodendron under the disturbance by rodents occurred in the total soluble sugar and reducing sugar contents that decreased in young plants,increased in middle-aged plants,and did not affect in mature plants.Crude protein and phosphorus contents significantly increased,while crude fiber and calcium contents significantly decreased in young plants.Crude fat and calcium contents significantly decreased in middle-aged plants.Soil organic matter(SOM),total nitrogen(TN),available nitrogen(AN)and available potassium(AK)contents in the topsoil(0–20 cm),which are conducive to forming''fertile islands'',also increased under the disturbance by rodents.In particular,soil AN and AK were the major factors affecting the above-ground morphological characteristics of H.ammodendron in burrowed areas.Overall,the response and defense strategies of H.ammodendron to the disturbance by rodents differed among different age groups,and the effect of the disturbance by rodents on H.ammodendron gradually weakened with the increasing plant age.
