One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase hi...One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase high performance liquid chromatography （HPLC）. The final purified fraction was checked on the Smart system HPLC and was judged as a pure fraction. The results of physical and biological analysis revealed that this fraction is heat stable and showed strong activities against Gram-positive bacterial growth. It possesses antibicrobial peptide properties and is worth further investigation.展开更多
基金Acknowledgments We thank Prof. X.-F. Pang, late Academician of Chinese Academy of Science, for his support in getting this project started. We also thank Prof. Yamakawa （National of Institute of Sericultural and Entomological Science, Japan） for analyzing the purity of the final purified fraction. We thank Dr H. Ma for his suggestions on this project and reading our manuscript. This work was supported partly by the Natural Science Foundation of Guangdong Province （032256）, the National Basic Research Program of China under grant 2005CB121000 and the National Natural Science Foundation （30370716）.
文摘One antibacterial activity fraction from an immunized dipteran insect, Bactrocera dorsalis, was isolated and purified by prepurification, ion-exchange chromatography, gel filtration chromatography and reverse-phase high performance liquid chromatography （HPLC）. The final purified fraction was checked on the Smart system HPLC and was judged as a pure fraction. The results of physical and biological analysis revealed that this fraction is heat stable and showed strong activities against Gram-positive bacterial growth. It possesses antibicrobial peptide properties and is worth further investigation.
