Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study...Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use efficiency of cotton to induce K deficiency.Results:The cotton variety,Jihe 321(wild type,WT)and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317,OE-29312)were studied in field with low soil-test K+(47.8 mg·kg^(−1)).In the field with low soil-test K+,only OE-29317 had less biomass and K+accumulation than the WT at some growth stages.Both Bt lines produced similar or even greater seed cotton yield than WT in the field.When the Bt gene(~70%)in OE-29317 and OE-29312 plants was silenced by virus-induced gene silencing(VIGS),the VIGS-Bt plants did not produce more biomass than VIGSgreen fluorescent protein(control)plants.Conclusions:The introduction of Bt gene did not necessarily hinder the K use efficiency of the cotton lines under this study.展开更多
Background:Cotton somatic embryogenesis is difficult or rarely frequent to present,which has limited gene function identification and biotechnological utility.Here,we employed a rice key somatic embryogenesis-related ...Background:Cotton somatic embryogenesis is difficult or rarely frequent to present,which has limited gene function identification and biotechnological utility.Here,we employed a rice key somatic embryogenesis-related gene,rice lesion simulating disease 1-like gene(OsLOL1),to develop transgenic cotton callus for evaluating its function in ectopic plants.Results:Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus,not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells.And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector.Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene(GhSOD1)expression,possibly resulting in reactive oxidant species(ROS)accumulation in transgenic callus cells.And OsLOL1-overexpressed embryogenic callus exhibited higherα-amylase activity compared with the control,resulting from the promotion of OsLOL1 to cotton amylase 7 gene(GhAmy7)and GhAmy8 expression.Conclusion:The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity,facilitating gene function analysis and molecular breeding in cotton.展开更多
文摘Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use efficiency of cotton to induce K deficiency.Results:The cotton variety,Jihe 321(wild type,WT)and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317,OE-29312)were studied in field with low soil-test K+(47.8 mg·kg^(−1)).In the field with low soil-test K+,only OE-29317 had less biomass and K+accumulation than the WT at some growth stages.Both Bt lines produced similar or even greater seed cotton yield than WT in the field.When the Bt gene(~70%)in OE-29317 and OE-29312 plants was silenced by virus-induced gene silencing(VIGS),the VIGS-Bt plants did not produce more biomass than VIGSgreen fluorescent protein(control)plants.Conclusions:The introduction of Bt gene did not necessarily hinder the K use efficiency of the cotton lines under this study.
基金the Natural Science Foundation of China(31971905 and 31771848)the State Key Laboratory of Cotton Biology Open Fund(CB2019B02).
文摘Background:Cotton somatic embryogenesis is difficult or rarely frequent to present,which has limited gene function identification and biotechnological utility.Here,we employed a rice key somatic embryogenesis-related gene,rice lesion simulating disease 1-like gene(OsLOL1),to develop transgenic cotton callus for evaluating its function in ectopic plants.Results:Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus,not only shortening time but also increasing transition of somatic callus cells to embryogenic callus cells.And the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty vector.Analysis of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene(GhSOD1)expression,possibly resulting in reactive oxidant species(ROS)accumulation in transgenic callus cells.And OsLOL1-overexpressed embryogenic callus exhibited higherα-amylase activity compared with the control,resulting from the promotion of OsLOL1 to cotton amylase 7 gene(GhAmy7)and GhAmy8 expression.Conclusion:The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity,facilitating gene function analysis and molecular breeding in cotton.
